Quantitative analysis of amino acids in human and bovine colostrum milk samples through iTRAQ labeling

2018 ◽  
Vol 98 (13) ◽  
pp. 5157-5163 ◽  
Author(s):  
Xiaona Liang ◽  
Hongjiao Han ◽  
Xue Zhao ◽  
Xueyan Cao ◽  
Mei Yang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Bovine Colostrum ◽  
Itraq Labeling ◽  
Milk Samples

Quantitative proteomic analysis of milk fat globule membrane (MFGM) proteins in human and bovine colostrum and mature milk samples through iTRAQ labeling

2016 ◽  
Vol 7 (5) ◽  
pp. 2438-2450 ◽  
Author(s):  
Mei Yang ◽  
Min Cong ◽  
Xiuming Peng ◽  
Junrui Wu ◽  
Rina Wu ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Milk Fat ◽  
Bovine Colostrum ◽  
Mature Milk ◽  
Milk Fat Globule Membrane ◽  
Quantitative Proteomic Analysis ◽  
Itraq Labeling ◽  
Milk Samples ◽  
Milk Fat Globule ◽  
Fat Globule

Milk fat globule membrane (MFGM) proteins have many functions.

scholarly journals QUANTITATIVE ANALYSIS OF BIOACTIVE COMPOUNDS OF ARTEMISIA NILAGIRICA (CLARKE) PAMP. LEAF EXTRACT

2016 ◽  
pp. 183
Author(s):  
Parameswari P ◽  
Devika Rengaswamy
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Quantitative Analysis ◽  
Secondary Metabolites ◽  
Bioactive Compounds ◽  
Leaf Extract ◽  
Metabolic Reaction ◽  
Quantitative Investigation ◽  
Artemisia Nilagirica ◽  
Quantitative Examination

<p>ABSTRACT<br />Objective: The points of this exploration work were to decide the quantitative examination of bioactive mixes. Customarily, cutting edge meds rely<br />on the phytochemicals got from the plant source in bigger extents. Numerous bioactive auxiliary metabolites have a positive metabolic reaction on<br />different human diseases.<br />Methods: In the present examination, Artemisia nilagirica, leaves were gathered, dried, powdered and put away in hermetically sealed compartments<br />for quantitative investigation of phytochemicals according to standard strategies.<br />Results: The methanolic leaf concentrate of enrolled 4.33 mg of alkaloids, 1.22 mg of saponins, 12.4 mg of tannins, 24.3 mg of glycosides, 10.2 mg<br />terpenoids, 1.33 mg of coumarin, 59.4 mg of amino acids, 12.2 mg of fatty acids, 17.2 mg of flavonoids, 10.2 mg of phenols, and steroids in follows<br />separately.<br />Conclusion: The plant has a high helpful quality as far as an assortment of phytochemicals from leaf remove and had let to a sure level toward<br />extraction and refinement of specific bioactive mixes for human nourishment.<br />Keywords: Artemisia nilagirica, Secondary metabolites, Quantitative analysis, Leaf extract, Flavonoids.</p>

scholarly journals Quantitative Analysis of the Whole-Body Metabolic Fate of Branched-Chain Amino Acids

2019 ◽  
Vol 29 (2) ◽  
pp. 417-429.e4 ◽  
Author(s):  
Michael D. Neinast ◽  
Cholsoon Jang ◽  
Sheng Hui ◽  
Danielle S. Murashige ◽  
Qingwei Chu ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Branched Chain Amino Acids ◽  
Whole Body ◽  
Metabolic Fate ◽  
Branched Chain

scholarly journals AMINO ACID COMPOSITION AND ELECTROPHORETIC PROPERTIES OF HYPERTENSIN I

1955 ◽  
Vol 102 (4) ◽  
pp. 435-440 ◽  
Author(s):  
Leonard T. Skeggs ◽  
Walton H. Marsh ◽  
Joseph R. Kahn ◽  
Norman P. Shumway
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Isoelectric Point ◽  
Column Chromatography ◽  
Isoleucine Leucine ◽  
Ion Exchange Column

A preparation of hypertensin I was purified by countercurrent distribution and was shown to migrate as a single component in starch blocks at pH 9.3 and 4.2. It had an isoelectric point of 7.7. Quantitative analysis by ion exchange column chromatography showed eight amino acids in approximately unimolar proportion: aspartic, proline, valine, isoleucine, leucine, tyrosine, phenylalanine, and arginine. There were in addition two moles of histidine.

Chromatographic Separation of Free Amino Acids in Table Sirups

1971 ◽  
Vol 54 (1) ◽  
pp. 61-65
Author(s):  
Arthur Russell Johnson ◽  
Richard L Corliss ◽  
Enrique Fernandez-Flores
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Amino Acid ◽  
Ion Exchange ◽  
Free Amino Acids ◽  
Free Amino ◽  
Amino Acid Mixture ◽  
Acid Mixture ◽  
Butyl Esters ◽  
Tlc Analysis

Abstract Qualitative chromatographic methods for the separation of free amino acids in table sirups are presented to aid in the development of chemical indices of composition which may be useful in establishing the identity of sirups and detecting their adulteration. Free amino acids in 2 table sirups were isolated on ion exchange columns and eluted with dilute ammonia. The concentrated amino acid mixture in the eluate was spotted directly on silica gel G plates for TLC analysis, or the amino acids were converted to their N-trifluoroacetyl n-butyl esters for GLC analysis. As many as 16 amino acids were qualitatively separated and identified and a potential for quantitative analysis was demonstrated.

Quantitative Analysis of Methionine, Cysteine, and Lysine in Feeds by Reversephase Liquid Chromatography Using Precolumn Derivatization with 9- Fluorenylmethyl Chloroformate: Preliminary Study

1990 ◽  
Vol 73 (6) ◽  
pp. 935-939 ◽  
Author(s):  
Angel Cubedo Fernández-Trapiella
Keyword(s):  
Amino Acids ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Performic Acid ◽  
Cysteic Acid ◽  
Precolumn Derivatization ◽  
Fluorescence Detector ◽  
Reverse Phase ◽  
Reverse Phase Liquid Chromatography ◽  
Phase Liquid

Abstract An Improved analytical method based on precolumn derivatization with 9-fluorenylmethyl chloroformate (9-FMC) and reverse- phase liquid chromatography was developed for quantitative analysis of methionine, cysteine, and lysine In feeds. Samples of corn, whey powder, soybean meal, meat meal, and fish meal were selected for an accurate determination of these 3 amino acids. A portion of each finely ground sample was weighed and subjected to oxidation with performic acid for 16 h before hydrolysis with 6N HCI for 24 h. An aliquot of each hydrolysate was evaporated, dissolved, and diluted with 0.2M pH 7.85 borate buffer. An aliquot of each final solution was derlvatlzed with 9-FMC and analyzed by reverse- phase liquid chromatography using a fluorescence detector with a 254 nm excitation filter and a 313 nm emission filter. The 2 sulfur amino acids and lysine were perfectly separated from all other amino acids with a simple binary gradient. Cysteine (analyzed as cysteic acid), methionine (as methionine sulfone), and lysine were quantltated using internal standard calibration. Hydrolysates were also analyzed by conventional Ion-exchange chromatography (IEC). Amino acid values as obtained by the proposed LC method were close to IEC data. Considering IEC results as reference values, the differences In recovery of amino acids In feedstuffs determined by both methods were not more than 7.5%. Precision of the LC method was evaluated within a single hydrolysate and between different hydrolysates of a single sample. Coefficients of variation (CV) were not more than 4.1 and 5.9%, respectively.

scholarly journals Absolute quantitative analysis of intact and oxidized amino acids by LC-MS without prior derivatization

Redox Biology ◽  
2020 ◽  
Vol 36 ◽  
pp. 101586 ◽  
Author(s):  
Luke F. Gamon ◽  
Chaorui Guo ◽  
Jianfei He ◽  
Per Hägglund ◽  
Clare L. Hawkins ◽  
...  
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis

QUANTITATIVE CHROMATOGRAPHIC METHODS: PART 7. ISOLATION OF AMINO ACIDS FROM SERUM AND OTHER FLUIDS

1961 ◽  
Vol 39 (2) ◽  
pp. 439-451 ◽  
Author(s):  
C. K. Harris ◽  
E. Tigane ◽  
C. S. Hanes
Keyword(s):  
Amino Acids ◽  
Quantitative Analysis ◽  
Ion Exchange ◽  
Paper Chromatography ◽  
Biological Fluids ◽  
Inorganic Cations ◽  
Chromatographic Methods ◽  
Tissue Extracts

A method based on the use of miniature ion-exchange columns has been devised for isolating small amounts of amino acids from biological fluids and tissue extracts. The amino acids are isolated virtually free from proteins, sugars, and inorganic cations in a single treatment.Techniques are described for the preparation of solutions of the isolated amino acids for quantitative analysis by paper chromatography.

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