Rapid screening ofAlternariamycotoxins using MALDI-TOF mass spectrometry

2016 ◽  
Vol 97 (1) ◽  
pp. 357-361 ◽  
Author(s):  
Kumaran Sivagnanam ◽  
Emy Komatsu ◽  
Christoph Rampitsch ◽  
Hélène Perreault ◽  
Tom Gräfenhan
Keyword(s):  
Mass Spectrometry ◽  
Rapid Screening ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Rapid Screening of Epidemiologically Important Salmonella enterica subsp. enterica Serovars by Whole-Cell Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

2011 ◽  
Vol 77 (12) ◽  
pp. 4136-4146 ◽  
Author(s):  
Ralf Dieckmann ◽  
Burkhard Malorny
Keyword(s):  
Mass Spectrometry ◽  
Salmonella Enterica ◽  
Laser Desorption ◽  
Rapid Screening ◽  
Laser Desorption Ionization ◽  
Ionization Time ◽  
Content Type ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Matrix Assisted Laser

ABSTRACTCurrently, 2,610 differentSalmonellaserovars have been described according to the White-Kauffmann-Le Minor scheme. They are routinely differentiated by serotyping, which is based on the antigenic variability at lipopolysaccharide moieties (O antigens), flagellar proteins (H1 and H2 antigens), and capsular polysaccharides (Vi antigens). The aim of this study was to evaluate the potential of matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectrometry for rapid screening and identification of epidemiologically importantSalmonella entericasubsp.entericaserovars based on specific sets of serovar-identifying biomarker ions. By analyzing 913Salmonella entericasubsp.entericastrains representing 89 different serovars using MALDI-TOF mass spectrometry, several potentially serovar-identifying biomarker ions were selected. Based on a combination of genus-, species-, subspecies-, and serovar-identifying biomarker ions, a decision tree classification algorithm was derived for the rapid identification of the five most frequently isolatedSalmonella entericaserovars, Enteritidis, Typhimurium/4,[5],12:i:-, Virchow, Infantis, and Hadar. Additionally, sets of potentially serovar-identifying biomarker ions were detected for other epidemiologically interesting serovars, such as Choleraesuis, Heidelberg, and Gallinarum. Furthermore, by using a bioinformatic approach, sequence variations corresponding to single or multiple amino acid exchanges in several biomarker proteins were tentatively assigned. The inclusivity and exclusivity of the specific sets of serovar-identifying biomarker ions for the top 5 serovars were almost 100%. This study shows that whole-cell MALDI-TOF mass spectrometry can be a rapid method for prescreeningS. entericasubsp.entericaisolates to identify epidemiologically important serovars and to reduce sample numbers that have to be subsequently analyzed using conventional serotyping by slide agglutination techniques.

scholarly journals Klebsiella MALDI TypeR: a web-based tool for Klebsiella identification based on MALDI-TOF mass spectrometry

2021 ◽  
pp. 103835
Author(s):  
Sébastien Bridel ◽  
Stephen C. Watts ◽  
Louise M. Judd ◽  
Taylor Harshegyi ◽  
Virginie Passet ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Web Based ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof
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