Characterization of bacterial community of raw milk from dairy cows during subacute ruminal acidosis challenge by high-throughput sequencing

2014 ◽  
Vol 95 (5) ◽  
pp. 1072-1079 ◽  
Author(s):  
Ruiyang Zhang ◽  
Wenjie Huo ◽  
Weiyun Zhu ◽  
Shengyong Mao
Keyword(s):  
Bacterial Community ◽  
Dairy Cows ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Raw Milk ◽  
Subacute Ruminal Acidosis ◽  
Ruminal Acidosis

Characterization of bacterial diversity in drinking water by pyrosequencing

2013 ◽  
Vol 13 (2) ◽  
pp. 358-367 ◽  
Author(s):  
Yan Zhang ◽  
Qiang He
Keyword(s):  
Drinking Water ◽  
Bacterial Community ◽  
Bacterial Diversity ◽  
Clone Library ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Community Composition ◽  
Environmental Distribution ◽  
Clone Library Analysis

Controlling microbial contamination of drinking water is critical to public health. However, understanding of the microbial ecology of drinking water remains incomplete. Representing the first application of high-throughput sequencing in drinking water microbiology, the objective of this study is to evaluate pyrosequencing as a high-throughput technique for the characterization of bacterial diversity in drinking water in comparison with conventional clone library analysis. Pyrosequencing and clone library analysis were performed in parallel to study the bacterial community composition in drinking water samples following the concentration of microbial biomass in drinking water with ultrafiltration. Validated by clone library analysis, pyrosequencing was confirmed as a highly efficient deep-sequencing technique to characterize the bacterial diversity in drinking water. Sequences of Alphaproteobacteria and Betaproteobacteria dominated the bacterial community in drinking water with Oxalobacteraceae and Methylobacteriaceae as the most abundant bacterial families, which is consistent with the prominent abundance of these populations frequently detected in various freshwater environments where source waters originate. Bacterial populations represented by the most abundant sequences in drinking water were closely related to cultures of metabolically versatile bacterial taxa widely distributed in the environment, suggesting a potential link between environmental distribution, metabolic characteristics, and abundance in drinking water.

scholarly journals Characterization of Bacterial Communities in Mexican Artisanal Raw Milk “Bola de Ocosingo” Cheese by High-Throughput Sequencing

2018 ◽  
Vol 9 ◽  
Author(s):  
Alejandro Aldrete-Tapia ◽  
Claudia Meyli Escobar-Ramírez ◽  
Mark L. Tamplin ◽  
Montserrat Hernández-Iturriaga
Keyword(s):  
High Throughput ◽  
Bacterial Communities ◽  
High Throughput Sequencing ◽  
Raw Milk

scholarly journals Characterization of oral bacterial diversity of irradiated patients by high-throughput sequencing

2013 ◽  
Vol 5 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Yue-Jian Hu ◽  
Qian Wang ◽  
Yun-Tao Jiang ◽  
Rui Ma ◽  
Wen-Wei Xia ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing
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