Breeding for seedless grapes using Chinese wild Vitis spp. II. In vitro embryo rescue and plant development

2013 ◽  
Vol 93 (15) ◽  
pp. 3870-3875 ◽  
Author(s):  
Wei Ji ◽  
Yuejin Wang
Keyword(s):  
Plant Development ◽  
Embryo Rescue ◽  
Chinese Wild Vitis ◽  
Seedless Grapes ◽  
Vitis Spp

Breeding of disease-resistant seedless grapes using Chinese wild Vitis spp.

2008 ◽  
Vol 117 (2) ◽  
pp. 136-141 ◽  
Author(s):  
Lili Tian ◽  
Yuejin Wang ◽  
Liang Niu ◽  
Dongmei Tang
Keyword(s):  
Chinese Wild Vitis ◽  
Disease Resistant ◽  
Seedless Grapes ◽  
Vitis Spp

scholarly journals In vitro embryo rescue and plant regeneration following self-pollination with irradiated pollen in cassava (Manihot esculenta Crantz)

2015 ◽  
Vol 14 (27) ◽  
pp. 2191-2201
Author(s):  
Buttibwa Mary ◽  
S Kawuki Robert ◽  
K Tugume Arthur ◽  
Akol Jacinta ◽  
Magambo Stephen ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Manihot Esculenta ◽  
Embryo Rescue ◽  
Manihot Esculenta Crantz ◽  
Irradiated Pollen ◽  
Self Pollination

scholarly journals Adaptability and leaf anatomical features in oil palm seedlings produced by embryo rescue and pre-germinated seeds

2010 ◽  
Vol 22 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Zanderluce G. Luis ◽  
Kadja Milena G. Bezerra ◽  
Jonny Everson Scherwinski-Pereira
Keyword(s):  
Oil Palm ◽  
Embryo Rescue ◽  
Morphological Characteristics ◽  
Growth Conditions ◽  
Epidermal Cells ◽  
In Vitro Cultivation ◽  
Vascular Bundles ◽  
Anatomical Plasticity ◽  
Germinated Seeds

Changes in the leaf structure of plants grown in different conditions have been reported, such as increase in size and density of stomata and reduction in stomatal control, amount of epicuticular wax, and mesophyll thickness, with a high diversity of intercellular spaces. However, these changes are highly variable depending on the physiological and morphological characteristics of each species. The objective of this work was to analyze the adaptability and anatomical plasticity of oil palm seedlings produced after embryo rescue and pre-germinated seeds. Expanded leaves were prepared for evaluation of morphometric data and anatomical structures. It was verified that the environmental conditions in vitro negatively influenced the stomata density, epidermal and hypodermal thickness, and the values for the expansion cells and leaf mesophile. Anatomically, the oil palm leaves present the same tissues composition in both growth conditions, with uniseriate epidermal cells, and tetracitic stomata occurring in both epidermal surfaces. Epidermal cells from in vitro plants are thinner than ones from greenhouse. The midrib of leaves from greenhouse plants are more developed and is composed by only one central vascular bundle, while plants from in vitro cultivation developed three to four collateral vascular bundles.

Nuclear DNA changes during plant development and the morphogenetic response in vitro of Nicotiana tabacum tissues

Plant Science ◽  
1987 ◽  
Vol 53 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M.M. Altamura ◽  
P. Bassi ◽  
A. Cavallini ◽  
G. Cionini ◽  
R. Cremonini ◽  
...  
Keyword(s):  
Nicotiana Tabacum ◽  
Plant Development ◽  
Nuclear Dna

scholarly journals Enhanced Seed Germination of Ilex dumosa R. (Aquifoliaceae) through In Vitro Culture of Cut Pyrenes

HortScience ◽  
2011 ◽  
Vol 46 (2) ◽  
pp. 278-281 ◽  
Author(s):  
Natalia R. Dolce ◽  
Luis A. Mroginski ◽  
Hebe Y. Rey
Keyword(s):  
In Vitro Culture ◽  
Embryo Rescue ◽  
Germination Percentage ◽  
Effective Technique ◽  
Cutting Surface ◽  
Rescue Technique ◽  
Growth Room ◽  
Scalpel Blade ◽  
Culture Protocol

An in vitro culture protocol was developed that increased the germination percentage and decreased the lag time to germination for Ilex dumosa R. pyrenes as a tool for replacing the laborious task of embryo rescue technique. This method involves transversely cutting surface-sterilized pyrenes with a scalpel blade, then placing the micropylar one-third end with the rudimentary embryo (≈0.25 mm long) on solidified (agar 0.65%) quarter-strength salts and vitamins of Murashige and Skoog, 1962 medium with 3% sucrose, and incubating in a growth room at 27 ± 2 °C with a 14-h photoperiod (116 μmol·m−2·s−1). Most of the cut pyrenes (greater than 50%) germinated within the first month after inoculation and achieved maximum germination (≈70%) in 2 months compared with whole pyrenes, which began to germinate 3 months after sowing and required more than 8 months for maximum germination (37%). Moreover, the germination percentage of cut pyrenes was significantly higher than the germination of isolated embryos (34%). Thus, the cut pyrenes culture is a simpler and more effective technique than embryo rescue. Easily, on average, a trained operator is able to culture ≈1000 cut pyrenes per day instead of ≈100 isolated embryos.

scholarly journals Embryo rescue from interspecific crosses in apple rootstocks

2006 ◽  
Vol 41 (6) ◽  
pp. 969-973 ◽  
Author(s):  
Adriana Cibele de Mesquita Dantas ◽  
José Itamar Boneti ◽  
Rubens Onofre Nodari ◽  
Miguel Pedro Guerra
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Embryo Rescue ◽  
Casein Hydrolysate ◽  
Female Parent ◽  
Interspecific Crosses ◽  
Open Pollination ◽  
Apple Rootstocks ◽  
Hand Pollination

The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from hand-pollination, cultivated in medium supplemented with 14 µM IAA, 5 µM kinetin and 1.5 µM Ga3 (MS1), mainly those of M. prunifolia x M. pumila, showed a high percentage of rusted embryos (96.2%). Embryos from open pollination of M. prunifolia and M. pumila formed calluses. It was possible to identify the influence of the female parent by the enhanced development of M. pumila shoots derived from open or hand-pollination. The crossing of responsive species and the use of the technique of embryo culture provided a rapid and uniform germination and, consequently, the development of fully normal seedlings.

Sign in / Sign up

Export Citation Format

Share Document