Microbial community structure in fermentation process of Shaoxing rice wine by Illumina-based metagenomic sequencing

2013 ◽  
Vol 93 (12) ◽  
pp. 3121-3125 ◽  
Author(s):  
Guangfa Xie ◽  
Lan Wang ◽  
Qikang Gao ◽  
Wenjing Yu ◽  
Xutao Hong ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Fermentation Process ◽  
Metagenomic Sequencing ◽  
Rice Wine

scholarly journals Genome-Centered Metagenomics Analysis Reveals the Microbial Interactions of a Syntrophic Consortium during Methane Generation in a Decentralized Wastewater Treatment System

2019 ◽  
Vol 10 (1) ◽  
pp. 135 ◽  
Author(s):  
Kun Zhang ◽  
Yan-Ling Zhang ◽  
Xin Ouyang ◽  
Jun-Peng Li ◽  
Jun-Jie Liao ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Community Structure ◽  
Microbial Community ◽  
Anaerobic Digestion ◽  
Microbial Community Structure ◽  
High Efficiency ◽  
Microbial Interactions ◽  
Clear Understanding ◽  
Metagenomic Sequencing ◽  
Decentralized Wastewater Treatment

The application of anaerobic digestors to decentralized wastewater treatment systems (DWTS) has gained momentum worldwide due to their ease of operation, high efficiency, and ability to recycle wastewater. However, the microbial mechanisms responsible for the high efficiency and ability of DWTS to recycle wastewater are still unclear. In this study, the microbial community structure and function of two different anaerobic bioreactors (a primary sludge digestor, PSD, and anaerobic membrane bioreactor, AnMBR) of a DWTS located in Germany was investigated using 16S rRNA gene amplicon and metagenomic sequencing, respectively. The results showed that the microbial community structure was remarkably different in PSD and AnMBR. Methanobacteriaceae and Syntrophaceae were identified as the families that significantly differed in abundance between these two bioreactors. We also used genome-centered metagenomics to predict the microbial interactions and methane-generating pathway, which yielded 21 near-complete assembled genomes (MAGs) (average completeness of 93.0% and contamination of 2.9%). These MAGs together represented the majority of the microbial community. MAGs affiliated with methanogenic archaea, including Methanobacterium sp., Methanomicrobiales archaea, Methanomassiliicoccales archaea, and Methanosaeta concilii, were recruited, along with other syntrophic bacterial MAGs associated with anaerobic digestion. Key genes encoding enzymes involved in specific carbohydrate-active and methanogenic pathways in MAGs were identified to illustrate the microbial functions and interactions that occur during anaerobic digestion in the wastewater treatment. From the MAG information, it was predicted that bacteria affiliated with Bacteroidetes, Prolixibacteraceae, and Synergistaceae were the key bacteria involved in anaerobic digestion. In the methane production step, Methanobacterium sp. performed hydrogenotrophic methanogenesis, which reduced carbon dioxide to methane with hydrogen as the primary electron donor. Taken together, our findings provide a clear understanding of the methane-generating pathways and highlight the syntrophic interactions that occur during anaerobic digestion in DWTS.

scholarly journals Taxonomic and Functional Metagenomics Profiling of Tuwa and Unnai Hot Springs Microbial Communities

2021 ◽  
Author(s):  
Disha Vora ◽  
Satyamitra Shekh ◽  
Madhvi Joshi ◽  
Amrutlal Patel ◽  
Chaitanya Joshi
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Hot Springs ◽  
Carbohydrate Binding ◽  
Metagenomic Sequencing ◽  
Bacterial Phyla ◽  
Functional Potential ◽  
Carbohydrate Binding Modules

Abstract Hot springs are of great importance due to their unique physicochemical properties. Due to unique selection pressure in this habitat, a diverse microbial community is prevailing and can be analyzed by high throughput sequencing technology. Present study focuses on metagenomic sequencing of two hot springs from Gujarat, India namely Tuwa and Unnai through both, culturable and culture independent approach. Sequence analysis from both the water reservoirs depicted higher species richness and diversity based on various diversity indices. The microbial community structure at both the hot springs was distinct and dependent on physicochemical factors like temperature, pH, mineral content etc. Enrichment by cultivation before metagenome sequencing revealed the abundance of Firmicutes (up to 96%) representing cultivable organisms in hotsprings. The bacterial phyla Firmicutes, Proteobacteria, Bacteroidetes, Thermotogae, Deinococcus-Thermus, and Chloroflexi dominate the thermoalkaline spring at Unnai and Tuwa in different proportion. Economically important microorganisms belonging to genera Thermus, Brevibacillus, Anoxybacillus, Bacillus, Pseudomonas, and Geobacillus were prevalent in hot springs. The analysis of functional potential by KEGG revealed pathways for metabolism of carbohydrates, amino acids, vitamins, cofactors and xenobiotics. Annotation with Carbohydrate Active EnZymes (CAZy) revealed the presence of four major classes of enzymes: glycosyl transferase, glycoside hydrolase, polysaccharide lyase and carbohydrate-binding modules. The study provides insight into the microbial community structure and their untapped functional potential for various biotechnological and environmental applications.

The First Use of Biofilm Microbial Community Structure as an Indicator of Impact of Two Dams on the Elwha River (Washington)

2009 ◽  
Vol 27 (4) ◽  
pp. 385-387
Author(s):  
W. D. Eaton ◽  
B. Wilmot ◽  
E. Epler ◽  
S. Mangiamelli ◽  
D. Barry
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Elwha River
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