Effects of Aspergillus spp. exogenous fibrolytic enzymes on in vitro fermentation of tropical forages

2012 ◽  
Vol 92 (12) ◽  
pp. 2569-2573 ◽  
Author(s):  
Fernanda DA Facchini ◽  
Victor RA Reis ◽  
Anna Paula Roth ◽  
Karla A Magalhães ◽  
Simone C Peixoto-Nogueira ◽  
...  
Keyword(s):  
In Vitro Fermentation ◽  
Fibrolytic Enzymes ◽  
Tropical Forages

scholarly journals Kinetic parameters of ruminal degradation in vitro with combinations of exogenous enzymes in diets of production systems simulated

2018 ◽  
Vol 19 (1) ◽  
pp. 69-82
Author(s):  
Lucien Bissi da Freiria ◽  
Joanis Tilemahos Zervoudakis ◽  
Nelcino Francisco de Paula ◽  
Luciano da Sival Cabral ◽  
Yasmim Rodrigues Vilas Boas ◽  
...  
Keyword(s):  
Kinetic Parameters ◽  
Production Systems ◽  
Gas Production ◽  
In Vitro Digestibility ◽  
In Vitro Fermentation ◽  
Ruminal Fluid ◽  
Fibrolytic Enzymes ◽  
Exogenous Enzymes ◽  
Santa Inês

SUMMARY This study evaluated the effects of eight combinations of three exogenous enzymes with fibrolytic activity (FIB), amylolytic activity (AMZ), and proteolytic activity (PRO) on the accumulated gas production (PG) and kinetic parameters in simulated fermentations in two diet production systems using the in vitro gas production technique. In experiment 1, ruminal fluid was obtained from two Santa Ines sheep, cannulated in the rumen, kept in a feedlot and fed a diet containing a roughage:concentrate ratio of 20:80. In experiment 2, the ruminal fluid was obtained from two Santa Ines sheep, cannulated in the rumen, and kept on pasture (Brachiaria brizantha cv. Marandu). The accumulated gas production was measured during a 96-hour incubation, measured at 18 different time points. After incubation, the pH, dry matter degradability, organic matter in vitro digestibility, digestibility of neutral detergent insoluble fiber, metabolizable energy, partitioning factor, gas yield, short chain fatty acids and microbial protein production were determined. In the in vitro fermentation using the feedlot diet, the increased use of fibrolytic enzymes increased PG and improved the profile characteristics and kinetic parameters of the fermentation, regardless of the combination with other enzymes. In the in vitro fermentation using the forage diet, increased fibrolytic enzymes also increased PG, with improvements in the kinetic parameters and the profile of the fermentation, independent of the use of the other enzymes. The addition only of fibrolytic enzymes has major potential to optimize the use of feedlot diets as well of forage in ruminant feed.

Fibrolytic enzymes improving in vitro rumen degradability of tropical forages

2020 ◽  
Vol 104 (5) ◽  
pp. 1267-1276
Author(s):  
Gabriel Zanuto Sakita ◽  
Thiago Francisco Ventoso Bompadre ◽  
Dhanasekaran Dineshkumar ◽  
Paulo de Mello Tavares Lima ◽  
Adibe Luiz Abdalla Filho ◽  
...  
Keyword(s):  
Fibrolytic Enzymes ◽  
Tropical Forages

scholarly journals Effect of exogenous fibrolytic enzymes on in vitro rumen fermentation of tropical forages

2004 ◽  
Vol 13 (Suppl. 1) ◽  
pp. 67-70
Author(s):  
L. Giraldo ◽  
M. Ranilla ◽  
M. Tejido ◽  
M. Carro
Keyword(s):  
Rumen Fermentation ◽  
Fibrolytic Enzymes ◽  
Tropical Forages

Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)

2002 ◽  
Vol 2002 ◽  
pp. 208-208 ◽  
Author(s):  
D. Colombatto ◽  
F. L. Mould ◽  
M. K. Bhat ◽  
E. Owen
Keyword(s):  
In Vitro Fermentation ◽  
Commercial Enzyme ◽  
Fibrolytic Enzymes ◽  
Optimum Activity ◽  
Ph Values ◽  
Optimum Ph ◽  
Ph Conditions ◽  
Ph Range ◽  
Optimum Ph Range

Modern feeding practices often lead to ruminal conditions being sub-optimal for fibre digestion. It has been speculated that fibrolytic enzymes, which usually show optimum activity at pH values below 6.0, may be of benefit when applied to diets of high producing animals. This study used a commercial enzyme mixture (EM), already identified as effective; to investigate its optimum pH range with respect to activity and its impact on the fermentation profiles of pure substrates, under differing pH conditions.

scholarly journals Preservation of Human Gut Microbiota Inoculums for In Vitro Fermentations Studies

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 14
Author(s):  
Nelson Mota de Carvalho ◽  
Diana Luazi Oliveira ◽  
Mayra Anton Dib Saleh ◽  
Manuela Pintado ◽  
Ana Raquel Madureira
Keyword(s):  
Gut Microbiota ◽  
Metabolic Profile ◽  
Bacterial Count ◽  
Glycerol Solution ◽  
Metabolic Profiles ◽  
Human Gut ◽  
Colonic Fermentation ◽  
In Vitro Fermentation ◽  
Fecal Samples

The use of fecal inoculums for in vitro fermentation models requires a viable gut microbiota, capable of fermenting the unabsorbed nutrients. Fresh samples from human donors are used; however, the availability of fresh fecal inoculum and its inherent variability is often a problem. This study aimed to optimize a method of preserving pooled human fecal samples for in vitro fermentation studies. Different conditions and times of storage at −20 °C were tested. In vitro fermentation experiments were carried out for both fresh and frozen inoculums, and the metabolic profile compared. In comparison with the fresh, the inoculum frozen in a PBS and 30% glycerol solution, had a significantly lower (p < 0.05) bacterial count (<1 log CFU/mL). However, no significant differences (p < 0.05) were found between the metabolic profiles after 48 h. Hence, a PBS and 30% glycerol solution can be used to maintain the gut microbiota viability during storage at −20 °C for at least 3 months, without interfering with the normal course of colonic fermentation.

Wheat cell walls and constituent polysaccharides induce similar microbiota profiles upon in vitro fermentation despite different short chain fatty acid end-product levels.

2021 ◽  
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Hong Yao ◽  
Barbara Williams ◽  
Bernadine Flanagan ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Cell Walls ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Plant Cell Walls ◽  
Human Gut ◽  
In Vitro Fermentation

Plant cell walls as well as their component polysaccharides in foods can be utilized to alter and maintain a beneficial human gut microbiota, but it is not known whether the...

Interaction of cellulose and xyloglucan influences in vitro fermentation outcomes

2021 ◽  
Vol 258 ◽  
pp. 117698
Author(s):  
Shiyi Lu ◽  
Deirdre Mikkelsen ◽  
Bernadine M. Flanagan ◽  
Barbara A. Williams ◽  
Michael J. Gidley
Keyword(s):  
In Vitro Fermentation
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