scholarly journals Erratum: Effect of fertilizer on functional properties of flour from four rice varieties grown in Sri Lanka

2011 ◽  
Vol 91 (9) ◽  
pp. 1728-1728
Author(s):  
Anil Gunaratne ◽  
Nihal Sirisena ◽  
Upul Kumari Ratnayaka ◽  
Jennet Ratnayaka ◽  
Xiangli Kong ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Functional Properties ◽  
Rice Varieties

Effect of fertiliser on functional properties of flour from four rice varieties grown in Sri Lanka

2011 ◽  
Vol 91 (7) ◽  
pp. 1271-1276 ◽  
Author(s):  
Anil Gunaratne ◽  
Nihal Sirisena ◽  
Upul Kumari Ratnayaka ◽  
Jennet Ratnayaka ◽  
Xiangli Kong ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Functional Properties ◽  
Rice Varieties

scholarly journals Validation of molecular markers for the analysis of genetic diversity of amylase content and gel consistency among representative rice varieties in Sri Lanka

2015 ◽  
Vol 26 (2) ◽  
pp. 317 ◽  
Author(s):  
H.K.D.H. Fernando ◽  
T.J.C. Kajenthini ◽  
S.P. Rebeira ◽  
T.C. Bamunuarachchige ◽  
H.A.M. Wickramasinghe
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Sri Lanka ◽  
Rice Varieties ◽  
Gel Consistency

scholarly journals DNA extraction technique for different rice varieties grown in Sri Lanka

2015 ◽  
Vol 3 (3) ◽  
pp. 398-401
Author(s):  
Ranganathan Kapilan
Keyword(s):  
Sri Lanka ◽  
Dna Extraction ◽  
Extraction Method ◽  
Extraction Methods ◽  
Rice Varieties ◽  
Modified Method ◽  
Dna Extraction Methods ◽  
Very High ◽  
Different Parts

Extraction of DNA is very important nowadays in bio-molecular researches. Extracted DNA should be purified and the quality of DNA should also be very high. The objective of the study was to develop a simple efficient method to isolate DNA from the rice varieties in an open laboratory environment, and to eliminate the usage of expensive chemicals and tools. The DNA extraction methods developed by the DNeasy plant kit method supplied by QIAGEN, Cheng et al., Doyle et al. and Michiels et al. were applied to five different rice varieties grown in different parts of Sri Lanka. Based on the quantity and quality of the extracted DNA tested by measuring the absorbance of DNA at 260 nm using Nanodrop® ND-1000 spectrophotometer and measuring the ratio of A260 / A280 and gel running on agarose, the efficiency of the extraction method chosen varied among rice varieties. Among the methods used, the methods introduced by DNeasy plant kit method supplied by QIAGEN and Cheng et al, yielded good and amplifiable quality DNA with satisfactory concentration for all the rice varieties tested. Therefore the modified method of Cheng et al, 1987 could be used to extract DNA from rice varieties instead of the commercially available expensive and hazardous DNeasy plant kit method supplied by QIAGEN.Int J Appl Sci Biotechnol, Vol 3(3): 398-401

scholarly journals Application of Pedimap — a pedigree visualization tool — to facilitate the decisioning of rice breeding in Sri Lanka

2020 ◽  
Author(s):  
P.G.R.G. Rathnayake ◽  
S.M. Sahibdeen ◽  
U.A.K.S. Udawela ◽  
C.K. Weebadde ◽  
W.M.W. Weerakoon ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Molecular Data ◽  
High Yield ◽  
Phenotypic Traits ◽  
Visualization Tool ◽  
Rice Breeding ◽  
Rice Varieties ◽  
Breeding Programs ◽  
Improved Varieties ◽  
Genomic Characterization

ABSTRACTThe development of rice cultivars with desirable traits is essential. The decision-making is a crucial step in rice breeding programs. The breeders can make efficient and pragmatic decisions if an organized pedigree visualization platform is available for the material of the rice breeding germplasm. The staple food in Sri Lanka is rice, and there is a great demand for improved varieties with high yield and other promising traits. In the present study, the available data of all the rice varieties released by Rice Research and Development Institute, Sri Lanka, and the related landraces and genotypes were arranged in Pedimap, a pedigree visualization tool. Pedimap can showcase pedigree relationships, phenotypic, and molecular data. The Identity by Descent (IBD) probabilities were calculated using FlexQTL software and included in the Pedimap database. The parentage selection based on the variations of phenotypic traits, selection of marker alleles for molecular breeding, and detection of the founders of genetic effects can be swiftly conducted using Pedimap. However, the power of harnessing the value of Pedimap for making breeding decisions relies on the availability of data for the traits, markers, and genomic sequences. Thus, it is imperative to characterize the breeding germplasms using standard phenomic and genomic characterization procedures before organized into Pedimap. Thereby, the worldwide breeding programs can benefit from each other to produce improved varieties to meet global challenges.

Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Shyama Weerakoon ◽  
Seneviratne Somaratne
Keyword(s):  
Genetic Diversity ◽  
Oryza Sativa ◽  
Sri Lanka ◽  
Core Collection ◽  
Morphological Characters ◽  
Morphological Features ◽  
Sri Lankan ◽  
Rice Varieties ◽  
Traditional Rice ◽  
Phenotypic And Genetic Diversity

Abstract. Weerakoon SR, Somaratne S. 2021. Development of a core collection from Sri Lankan traditional rice (Oryza sativa) varieties for phenotypic and genetic diversity. Nusantara Bioscience 13: 61-67. A collection of over 2000 traditional rice varieties are conserved at Gene Bank, Plant Genetics Resource Center, Sri Lanka. Oryza sativa varieties grown in Sri Lanka from ancient times to the middle of the last century are known as traditional rice. These varieties show adaptability to biotic and abiotic stresses and, an important component of biodiversity of Sri Lanka. A detailed understanding of the diversity of traditional rice varieties is essential for effective utilization of rice genetic resources and identification of potential parents possessing valuable genetic traits for future crop improvement. Study objectives were phenotypic and molecular characterization of one-hundred traditional rice varieties and to identify a core collection for phenotypic and genetic diversity. Rice varieties were grown in a plant house following RCBD with 4 replicates and 5 plants per replicate. Thirty-two agro-morphological characters were observed/collected. Genomic DNA was extracted from 20-days-old seedlings. Thirty?three microsatellite (Simple Sequence Repeat-SSR) primer pairs were used to assay genetic variation and PCR products were subjected to fragment analysis by capillary electrophoresis. Descriptive statistics and basic inferential statistical analyses were performed to access variation of agro-morphological characters among rice varieties. Cluster analysis and Multidimensional scaling produced 07 groups which were further analyzed using Classification and Regression Analysis to extract the diagnostic agro-morphological features. Groups of rice varieties were characterized by lemma palea color, awn color at maturity, seedling height, and flag-leaf angle. Traditional varieties represent distant clusters on agro-morphological features. Molecular analyses revealed all 33 loci displayed polymorphism (66.7-96.9%) among 100 traditional rice varieties with a total of 387 alleles identified with an average of 11.72 alleles per variety. All varieties were genetically structured into fifteen well-separated groups. UPGMA analysis based on Jaccard's similarity separated varieties into 05 major clusters. Genetic diversity information is useful in the efficient use of Sri Lankan rice germplasm and managing in situ and ex situ germplasm collections in conserving traditional rice varieties.

Rheology and functional properties of starches isolated from five improved rice varieties from West Africa

2011 ◽  
Vol 25 (7) ◽  
pp. 1785-1792 ◽  
Author(s):  
Olayide S. Lawal ◽  
Romano Lapasin ◽  
Barbara Bellich ◽  
Tajudeen O. Olayiwola ◽  
Attilio Cesàro ◽  
...  
Keyword(s):  
West Africa ◽  
Functional Properties ◽  
Rice Varieties

scholarly journals Grain Amylose Content and its Stability over Seasons in a Selected Set of Rice Varieties Grown in Sri Lanka

2017 ◽  
Vol 12 (1) ◽  
pp. 43 ◽  
Author(s):  
W. K. S. M. Abeysekera ◽  
G. A. S. Premakumara ◽  
A. P. Bentota ◽  
D. Sumith De Z. Abeysiriwardena
Keyword(s):  
Sri Lanka ◽  
Amylose Content ◽  
Rice Varieties
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