Comparative study of in vitro digestibility of major allergen tropomyosin and other food proteins of Chinese mitten crab (Eriocheir sinensis)

2010 ◽  
Vol 90 (10) ◽  
pp. 1614-1620 ◽  
Author(s):  
Guang-Ming Liu ◽  
Min-Jie Cao ◽  
Yuan-Yuan Huang ◽  
Qiu-Feng Cai ◽  
Wu-Yin Weng ◽  
...  
Keyword(s):  
Comparative Study ◽  
Major Allergen ◽  
Eriocheir Sinensis ◽  
In Vitro Digestibility ◽  
Chinese Mitten Crab ◽  
Food Proteins ◽  
Mitten Crab

Comparative study on the nutrition composition of adult male Chinese mitten crab ( Eriocheir sinensis ) with different coloured hepatopancreases

2020 ◽  
Vol 52 (1) ◽  
pp. 196-207
Author(s):  
Qiu‐ju Wang ◽  
Xiao‐dong Jiang ◽  
Qi Yao ◽  
Xiao‐wen Long ◽  
Yong‐ping Xu ◽  
...  
Keyword(s):  
Comparative Study ◽  
Adult Male ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals An inhibitor of apoptosis protein (EsIAP1) from Chinese mitten crab Eriocheir sinensis regulates apoptosis through inhibiting the activity of EsCaspase-3/7-1

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Chen Qu ◽  
Jiejie Sun ◽  
Qingsong Xu ◽  
Xiaojing Lv ◽  
Wen Yang ◽  
...  
Keyword(s):  
Caspase 3 ◽  
Hydrolytic Activity ◽  
Eriocheir Sinensis ◽  
Inhibitor Of Apoptosis ◽  
Chinese Mitten Crab ◽  
Apoptosis Pathway ◽  
Mitten Crab ◽  
Apoptosis Protein ◽  
Apoptosis Proteins

AbstractInhibitor of apoptosis proteins (IAPs) maintain the balance between cell proliferation and cell death by inhibiting caspase activities and mediating immune responses. In the present study, a homolog of IAP (designated as EsIAP1) was identified from Chinese mitten crab Eriocheir sinensis. EsIAP1 consisted of 451 amino acids containing two baculoviral IAP repeat (BIR) domains with the conserved Cx2 Cx6 Wx3 Dx5 Hx6 C motifs. EsIAP1 mRNA was expressed in various tissues and its expression level in hemocytes increased significantly (p < 0.01) at 12–48 h after lipopolysaccharide stimulation. In the hemocytes, EsIAP1 protein was mainly distributed in the cytoplasm. The hydrolytic activity of recombinant EsCaspase-3/7-1 against the substrate Ac-DEVD-pNA decreased after incubation with rEsIAP1. Moreover, rEsIAP1 could directly combine with rEsCaspase-3/7-1 in vitro. After EsIAP1 was interfered by dsRNA, the mRNA expression and the hydrolytic activity of EsCaspase-3/7-1 increased significantly, which was 2.26-fold (p < 0.05) and 1.71-fold (p < 0.05) compared to that in the dsGFP group, respectively. These results collectively demonstrated that EsIAP1 might play an important role in apoptosis pathway by regulating the activity of EsCaspase-3/7-1 in E. sinensis.

Establishment of spiroplasma-infected hemocytes as an in vitro laboratory culture model of Chinese mitten crab Eriocheir sinensis

2014 ◽  
Vol 171 (1-2) ◽  
pp. 215-220 ◽  
Author(s):  
Wei Gu ◽  
Wei Yao ◽  
Yang zhao ◽  
Siyu Pei ◽  
Chenkang Jiang ◽  
...  
Keyword(s):  
Eriocheir Sinensis ◽  
Laboratory Culture ◽  
Chinese Mitten Crab ◽  
Mitten Crab ◽  
Culture Model

ERK Is Involved in the Process of Acrosome Reaction In Vitro of the Chinese Mitten Crab, Eriocheir sinensis

2015 ◽  
Vol 17 (3) ◽  
pp. 305-316 ◽  
Author(s):  
Wen-Juan Sun ◽  
Ming Zhu ◽  
Yuan-Li Wang ◽  
Qing Li ◽  
Hong-Dan Yang ◽  
...  
Keyword(s):  
Acrosome Reaction ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab

scholarly journals Effects of miR-143 and its target receptor 5-HT2B on agonistic behavior in the Chinese mitten crab (Eriocheir sinensis)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yang-Yang Pang ◽  
Gen-Yong Huang ◽  
Ya-Meng Song ◽  
Xiao- Zhe Song ◽  
Jia-Huan Lv ◽  
...  
Keyword(s):  
Agonistic Behavior ◽  
Cost Effective ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Physical Damage ◽  
Phylogenetic Tree Analysis ◽  
Important Species ◽  
Downstream Target ◽  
Mitten Crab

AbstractChinese mitten crab (Eriocheir sinensis) as a commercially important species is widely cultured in China. However, E. sinensis is prone to agonistic behavior, which causes physical damage and wastes energy resources, negatively impacting their growth and survival. Therefore, understanding the regulatory mechanisms that underlie the switching of such behavior is essential for ensuring the efficient and cost-effective aquaculture of E. sinensis. The 5-HT2B receptor is a key downstream target of serotonin (5-HT), which is involved in regulating animal behavior. In this study, the full-length sequence of 5-HT2B gene was cloned. The total length of the 5-HT2B gene was found to be 3127 bp with a 236 bp 5′-UTR (untranslated region), a 779 bp 3′-UTR, and a 2112 bp open reading frame encoding 703 amino acids. Phylogenetic tree analysis revealed that the 5-HT2B amino acid sequence of E. sinensis is highly conserved with that of Cancer borealis. Using in vitro co-culture and luciferase assays, the miR-143 targets the 5-HT2B 3′-UTR and inhibits 5-HT2B expression was confirmed. Furthermore, RT-qPCR and Western blotting analyses revealed that the miR-143 mimic significantly inhibits 5-HT2B mRNA and protein expression. However, injection of miR-143 did not decrease agonistic behavior, indicating that 5-HT2B is not involved in the regulation of such behavior in E. sinensis.

scholarly journals Comparative Study of in Vitro Digestibility of Food Proteins and Effect of Preheating on the Digestion

2003 ◽  
Vol 26 (7) ◽  
pp. 969-973 ◽  
Author(s):  
Kayoko Takagi ◽  
Reiko Teshima ◽  
Haruyo Okunuki ◽  
Jun-ichi Sawada
Keyword(s):  
Comparative Study ◽  
In Vitro Digestibility ◽  
Food Proteins

Identification and characterisation of the major allergen of Chinese mitten crab (Eriocheir sinensis)

2008 ◽  
Vol 111 (4) ◽  
pp. 998-1003 ◽  
Author(s):  
Yin-Long Liang ◽  
Min-Jie Cao ◽  
Wen-Jin Su ◽  
Ling-Jing Zhang ◽  
Yuan-Yuan Huang ◽  
...  
Keyword(s):  
Major Allergen ◽  
Eriocheir Sinensis ◽  
Chinese Mitten Crab ◽  
Mitten Crab
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