Characterization of α-tubulin gene distinctively presented in a cytoplasmic male sterile and its maintainer line of non-heading Chinese cabbage

2009 ◽  
Vol 89 (2) ◽  
pp. 274-280 ◽  
Author(s):  
Jingyi Zhang ◽  
Ying Li ◽  
Gongjun Shi ◽  
Xiaofeng Chen ◽  
Jianjun Wang ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Male Sterile ◽  
Tubulin Gene ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Heading Chinese Cabbage

Microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading Chinese cabbage

2012 ◽  
Vol 92 (15) ◽  
pp. 3046-3054 ◽  
Author(s):  
Jingyi Zhang ◽  
Changwei Zhang ◽  
Yan Cheng ◽  
Li Qi ◽  
Shumin Wang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Chinese Cabbage ◽  
Male Sterile ◽  
Male Sterile Line ◽  
Cytoplasmic Male Sterile ◽  
Heading Chinese Cabbage

Combining ability and heterosis analysis for mineral elements by using cytoplasmic male-sterile systems in non-heading Chinese cabbage (Brassica rapa)

2018 ◽  
Vol 69 (3) ◽  
pp. 296 ◽  
Author(s):  
Fei Xie ◽  
Jun Zha ◽  
Hongyu Tang ◽  
Yuying Xu ◽  
Xujia Liu ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Combining Ability ◽  
Nutritional Quality ◽  
Mineral Elements ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Positive Effects ◽  
Cytoplasmic Effects ◽  
Cms Line ◽  
Heading Chinese Cabbage

Brassica vegetables are an important source of dietary nutrition. The nutritional quality of mineral elements is becoming one of the most important studied traits because of the year-round supply of vegetables in China. However, there are few reports about breeding and utilisation of mineral elements in non-heading Chinese cabbage (Brassica napus L.). Using two newly reported CMS (cytoplasmic male-sterile) lines of non-heading Chinese cabbage, we conducted incomplete diallel experiments to analyse heterosis, combining ability and cytoplasmic effects for mineral elements such as calcium (Ca), iron (Fe), magnesium (Mg) and zinc (Zn). Heterosis analysis of mineral elements indicated that the crossing combinations A1 (hau CMS) × C03, A2 (eru CMS) × C03 and A2 × C11 exhibited desirable positive effects of mid-parent heterosis and high-parent heterosis in terms of mineral element content that could be exploited for commercial purposes. Analysis of general combining ability (GCA) effects of the parental lines indicated that the tester C11 was superior for the improvement of the four mineral elements; CMS line A1 had greater GCA effects than CMS line A2 for Ca and Fe. The hybrid combinations A2 × C11, B × C05 and B × C12 showed positive specific combining ability (SCA) effects for the four mineral elements on overall performance. The analysis revealed that cytoplasmic effects of hau CMS and eru CMS were both positive for Ca and Fe, and that A1 had more obvious cytoplasmic effects than did A2. These results indicated that the two isonuclear, alloplasmic CMS lines of non-heading Chinese cabbage might be useful for improving the nutritional quality traits of cruciferous vegetables and for heterosis utilisation.

scholarly journals Changes of Endogenous Hormones and Polyamines in Cytoplasmic Male Sterile Non-heading Chinese Cabbage During Anther Development

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 881D-881
Author(s):  
Gong-Jun Shi* ◽  
Xi-Lin Hou ◽  
Wei Hu ◽  
Zhong-Chun Jiang
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Anther Development ◽  
Endogenous Hormones ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Heading Chinese Cabbage

It studies the changes of endogenous hormones and polyamines in cytoplasmic male sterile non-heading Chinese cabbage (Brassica campestris L. ssp. chinensis Makino var. communis Tsen et Lee). Results showed that the microspore was prone to being sterile when there were lack of IAA, GA and polyamines, especially Put and abundant with ZRs and ABA in the anther. The imbalance of IAA/ZRs also easily caused the anther sterile.

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Study on the expression of specific chimeric-orfs of C type cytoplasmic male sterile in maize

2013 ◽  
Vol 61 (1-4) ◽  
pp. 25-36 ◽  
Author(s):  
Jiyue Wang ◽  
Lihua Zeng ◽  
Shengqing Wang ◽  
Jing Wang ◽  
Yanli Lu ◽  
...  
Keyword(s):  
Base Substitution ◽  
Pcr Analysis ◽  
Male Sterile ◽  
Maintainer Line ◽  
Cytoplasmic Male Sterile ◽  
Real Time Quantitative Pcr ◽  
Substitution Mutations ◽  
Membrane Spanning ◽  
Insertion Mutations ◽  
Online Software

In the present study, a mutated chimericorf364, namedorf366-c, was identified in a maize C type cytoplasmic male sterile line (CMS-C) by PCR and RT-PCR. Four base pair adjacent base substitution mutations (CAAA to TTTT) and eight bp insertion mutations were found inorf366-ccompared withorf364. ORF366-C was predicted to contain one membrane-spanning domain using TMHMM online software. Real-time quantitative PCR analysis showed thatorf366-cwas upregulated in the CMS-C line in comparison to its maintainer line at the uninucleate stage. The protokaryotic protein expression oforf366-cinEscherichia colishowed that ORF366-C could be a cytotoxic protein. All the results indicated thatorf366-cmay be associated with maize CMS-C.

Sign in / Sign up

Export Citation Format

Share Document