Development and validation of gas chromatography and real-time quantitative PCR for the quantification of landscape-scale gene flow from varieties of high erucic acid (HEAR) oilseed rape

2008 ◽  
Vol 88 (13) ◽  
pp. 2253-2264 ◽  
Author(s):  
Danny W Cullen ◽  
Geoffrey R Squire ◽  
Jim W McNicol ◽  
Jennifer H Jacobs ◽  
Juliet L Osborne ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Gene Flow ◽  
Erucic Acid ◽  
Real Time ◽  
Quantitative Pcr ◽  
Oilseed Rape ◽  
Landscape Scale ◽  
High Erucic Acid ◽  
Real Time Quantitative Pcr ◽  
Development And Validation

Development and validation of a real-time quantitative PCR assay to detect Xanthomonas axonopodis pv. allii from onion seed

2015 ◽  
Vol 114 ◽  
pp. 78-86 ◽  
Author(s):  
Isabelle Robène ◽  
Marion Perret ◽  
Emmanuel Jouen ◽  
Aline Escalon ◽  
Marie-Véronique Maillot ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Pcr Assay ◽  
Xanthomonas Axonopodis ◽  
Real Time Quantitative Pcr ◽  
Onion Seed ◽  
Development And Validation ◽  
Quantitative Pcr Assay

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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