scholarly journals A simple DNA extraction method suitable for PCR detection of genetically modified maize

2007 ◽  
Vol 87 (14) ◽  
pp. 2728-2731 ◽  
Author(s):  
Manuel Porcar ◽  
Silvia Ramos ◽  
Amparo Latorre
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Genetically Modified ◽  
Pcr Detection ◽  
Genetically Modified Maize ◽  
Dna Extraction Method

A rapid genomic DNA extraction method and its combination with helicase dependent amplification for the detection of genetically modified maize

2016 ◽  
Vol 8 (1) ◽  
pp. 136-141 ◽  
Author(s):  
Eric Gonzalez Garcia ◽  
Andreas H. Farnleitner ◽  
Robert L. Mach ◽  
Rudolf Krska ◽  
Kurt Brunner
Keyword(s):  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Solvent Free ◽  
Genetically Modified Maize ◽  
Genomic Dna Extraction ◽  
Dna Extraction Method

A simple, solvent-free extraction method for DNA facilitates the analysis for genetically modified organisms.

scholarly journals A DNA Extraction Method Using a Silica-base Resin Type Kit for the Detection of Genetically Modified Papaya

2008 ◽  
Vol 49 (2) ◽  
pp. 63-69 ◽  
Author(s):  
Kiyomi OHMORI ◽  
Hisayo TSUCHIYA ◽  
Takahiro WATANABE ◽  
Hiroshi AKIYAMA ◽  
Tamio MAITANI ◽  
...  
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Genetically Modified ◽  
Dna Extraction Method ◽  
Base Resin

Sensitivity detection of Abacavir in human through SNP detection of HLA-B*5701 allele

2016 ◽  
Vol 5 (08) ◽  
pp. 4754
Author(s):  
Tanushree Mitra* ◽  
Shivshankar Kumdale ◽  
Sameer Chowdhary ◽  
Amol D. Raut
Keyword(s):  
Blood Sample ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Extraction Method ◽  
Snp Detection ◽  
Dna Extraction Method ◽  
Sensitivity Detection

The main objective of this study was to make sure whether randomly taken 12 samples were sensitive to abacavir. The genomic DNA from 12 blood sample were extracted by phenol chloroform DNA extraction method, extracted genomic DNA were amplified and sequenced, thereafter SNPs were detected. Every sample had shown the presence of normal base at SNP position. This study indicated, those randomly taken 12 patients were sensitive to abacavir, so they can consume abacavir if they get infected with HIV.

Development of a rapid DNA extraction method and one-step nested PCR for the detection of Naegleria fowleri from the environment

2011 ◽  
Vol 45 (16) ◽  
pp. 5211-5217 ◽  
Author(s):  
Arine Fadzlun Ahmad ◽  
James Lonnen ◽  
Peter W. Andrew ◽  
Simon Kilvington
Keyword(s):  
Dna Extraction ◽  
Nested Pcr ◽  
Extraction Method ◽  
Naegleria Fowleri ◽  
Dna Extraction Method ◽  
One Step ◽  
Nægleria Fowleri

scholarly journals Choice of bacterial DNA extraction method from fecal material influences community structure as evaluated by metagenomic analysis

Microbiome ◽  
2014 ◽  
Vol 2 (1) ◽  
pp. 19 ◽  
Author(s):  
Agata Wesolowska-Andersen ◽  
Martin Bahl ◽  
Vera Carvalho ◽  
Karsten Kristiansen ◽  
Thomas Sicheritz-Pontén ◽  
...  
Keyword(s):  
Community Structure ◽  
Dna Extraction ◽  
Extraction Method ◽  
Metagenomic Analysis ◽  
Fecal Material ◽  
Bacterial Dna ◽  
Dna Extraction Method ◽  
Bacterial Dna Extraction

scholarly journals Evaluating the Impact of DNA Extraction Method on the Representation of Human Oral Bacterial and Fungal Communities

PLoS ONE ◽  
2017 ◽  
Vol 12 (1) ◽  
pp. e0169877 ◽  
Author(s):  
Anna Vesty ◽  
Kristi Biswas ◽  
Michael W. Taylor ◽  
Kim Gear ◽  
Richard G. Douglas
Keyword(s):  
Dna Extraction ◽  
Extraction Method ◽  
Fungal Communities ◽  
Dna Extraction Method ◽  
The Impact

scholarly journals Optimized Protocol for Cyanobacterial 16S rRNA Analysis in Danube Delta Lakes

2021 ◽  
Author(s):  
Maria Iasmina Moza ◽  
Carmen Postolache
Keyword(s):  
16S Rrna ◽  
Dna Extraction ◽  
Phytoplankton Biomass ◽  
Extraction Method ◽  
Seasonal Difference ◽  
Danube Delta ◽  
16S Rrna Analysis ◽  
Dna Extraction Method ◽  
Optimized Protocol ◽  
Cyanobacterial Biomass

AbstractMolecular biology protocols have been more and more accessible to researchers for ecological investigations, however, these protocols always require optimization steps for the analysis of specific types of samples. The purpose of this study was to optimize a molecular protocol for the analysis of cyanobacterial 16S rRNA in Danube Delta shallows lakes. In this regard, several commercial DNA extraction kits were tested in comparison with potassium ethyl xanthogenate extraction method on different matrices. The obtained DNA was further used for 16S rRNA PCR optimization. Finally, an optimized protocol is proposed for the molecular analysis of cyanobacteria group in freshwater samples. The best DNA extraction method was the potassium xanthogenate extraction from dried cyanobacterial biomass. A dynamic in total genomic eDNA was observed, reflecting the seasonal difference in phytoplankton biomass from the studied lakes. The PCR protocol optimized by us can be successfully applied for the identification of a broad range of cyanobacterial genetic markers.

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