Determination of thiobarbituric acid reactive substances (TBARS) in fish tissue by an improved distillation–spectrophotometric method

1984 ◽  
Vol 35 (11) ◽  
pp. 1248-1254 ◽  
Author(s):  
P. J. Ke ◽  
E. Cervantes ◽  
C. Robles-Martinez
Keyword(s):  
Spectrophotometric Method ◽  
Thiobarbituric Acid ◽  
Fish Tissue ◽  
Thiobarbituric Acid Reactive Substances

scholarly journals A rapid spectrophotometric method for determination of thiobarbituric acid reactive substances in rainbow trout feed

2020 ◽  
Vol 47 (1) ◽  
pp. 43-53
Author(s):  
Slađana Rakita ◽  
Dušica Čolović ◽  
Alenka Levart ◽  
Vojislav Banjac ◽  
Radmilo Čolović ◽  
...  
Keyword(s):  
Rainbow Trout ◽  
Spectrophotometric Method ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances

scholarly journals A Simple Spectrophotometric Method for the Determination of Thiobarbituric Acid Reactive Substances in Fried Fast Foods

2016 ◽  
Vol 2016 ◽  
pp. 1-5 ◽  
Author(s):  
Alam Zeb ◽  
Fareed Ullah
Keyword(s):  
Acetic Acid ◽  
Spectrophotometric Method ◽  
Glacial Acetic Acid ◽  
Thiobarbituric Acid ◽  
Potato Chips ◽  
Thiobarbituric Acid Reactive Substances ◽  
Acetic Acid Medium ◽  
Fast Foods ◽  
Acetic Acid Water

A simple and highly sensitive spectrophotometric method was developed for the determination of thiobarbituric acid reactive substances (TBARS) as a marker for lipid peroxidation in fried fast foods. The method uses the reaction of malondialdehyde (MDA) and TBA in the glacial acetic acid medium. The method was precise, sensitive, and highly reproducible for quantitative determination of TBARS. The precision of extractions and analytical procedure was very high as compared to the reported methods. The method was used to determine the TBARS contents in the fried fast foods such as Shami kebab, samosa, fried bread, and potato chips. Shami kebab, samosa, and potato chips have higher amount of TBARS in glacial acetic acid-water extraction system than their corresponding pure glacial acetic acid and vice versa in fried bread samples. The method can successfully be used for the determination of TBARS in other food matrices, especially in quality control of food industries.

Distribution of thiobarbituric acid-reactive substances in lipoproteins and proteins in serum.

1989 ◽  
Vol 35 (10) ◽  
pp. 2054-2058 ◽  
Author(s):  
D Bonnefont ◽  
A Legrand ◽  
J Peynet ◽  
J Emerit ◽  
J Delattre ◽  
...  
Keyword(s):  
Apolipoprotein B ◽  
Thiobarbituric Acid ◽  
Precipitation Method ◽  
High Density Lipoproteins ◽  
Separation Procedure ◽  
Serum Lipoproteins ◽  
Thiobarbituric Acid Reactive Substances ◽  
Selective Precipitation ◽  
Acid Reaction

Abstract We assessed the distribution of malondialdehyde (MDA) in lipoproteins and proteins in serum after using two procedures to separate the lipoproteins: sequential ultracentrifugation or selective precipitation with a sodium phosphotungstate and magnesium chloride reagent followed by ultracentrifugation of the supernate. MDA concentrations were determined by the thiobarbituric acid reaction and quantified by fluorometry. We found that 43% of the thiobarbituric acid-reactive substances (TBARS) was bound to the lipoproteins--27% to very-low- and low-density lipoproteins (VLDL-LDL) and 16% to high-density lipoproteins (HDL)--and from 11.5% to 15.8% to proteins, depending on the separation procedure. Residual unbound TBARS were located in the ultracentrifugation layers that contained no lipoproteins or proteins. The TBARS concentration in serum lipoproteins containing apolipoprotein B (i.e., VLDL-LDL) was the same after ultracentrifugation or selective precipitation. We therefore consider the precipitation method more suitable for routine TBARS determination in these lipoproteins, because it is easier to handle and faster. However, for determination of TBARS in HDL, selective precipitation requires subsequent ultracentrifugation at a density of 1.21 kg/L.

Optimized steps in fluorometric determination of thiobarbituric acid-reactive substances in serum: importance of extraction pH and influence of sample preservation and storage

1993 ◽  
Vol 39 (12) ◽  
pp. 2522-2526 ◽  
Author(s):  
W Wasowicz ◽  
J Nève ◽  
A Peretz
Keyword(s):  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Fluorometric Method ◽  
C Storage ◽  
Insulin Dependent ◽  
The Mean ◽  
Optimized Conditions ◽  
And Storage

Abstract A simple, reliable, and reproducible fluorometric method for measuring thiobarbituric acid-reactive substances (TBARS) in serum is proposed, based on the reaction between malondialdehyde (MDA) and thiobarbituric acid. Formation of TBARS was complete at pH 2.4-2.6, but extraction with n-butanol proved complete only at lower pH, i.e., 1.6-1.7. Analytical recoveries of MDA added to serum were 94%-101%; within- and between-run CVs were 2.4-3.6% and 4.6-5.5%; and the detection limit for TBARS in serum was 0.10 mumol/L. Optimized conditions included: (a) collection of either serum or heparinized plasma, (b) preservation from in vitro autoxidation by glutathione and EDTA, and (c) storage at -20 degrees C up to 35 days. The mean (+/- SD) TBARS concentration in 47 healthy adults was 1.01 (0.21) mumol/L; no sex-related difference was observed. Higher concentrations were measured in patients with renal insufficiency undergoing hemodialysis and in patients with insulin-dependent diabetes, chronic pancreatitis, or liver cirrhosis.

Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

2018 ◽  
Vol 69 (7) ◽  
pp. 1860-1865
Author(s):  
Luciana Teodora Rotaru ◽  
Renata Maria Varut ◽  
Mihai Banicioiu Covei ◽  
Irina Iuliana Costache ◽  
Marius Novac ◽  
...  
Keyword(s):  
Lipid Peroxides ◽  
Thiobarbituric Acid ◽  
Vaccinium Myrtillus ◽  
Tamarix Ramosissima ◽  
Diabetic Mice ◽  
Flavonoid Content ◽  
Thiobarbituric Acid Reactive Substances ◽  
Total Polyphenol

Tamarix ramosissima (Tamaricaceae) is a small tree that grows spontaneously in Europe and Asia, being considered an invasive species in geographical areas with warm climates. The chemical composition is partially elucidated, being empirically used for antiinflammatory, analgesic, antibacterial and antioxidant effect. Our study aimed to evaluate the total polyphenol and flavonoid content of vegetal extracts and to test in vivo antioxidant therapeutic effect of it, comparative with Vaccinium myrtillus, using streptozotocin-induced diabetic mice. After five weeks the animals were sacrificed and we determined erythrocyte activities of superoxide dismutase, glutathione peroxidase, glutathione reductase and level of lipid peroxides as thiobarbituric acid reactive substances. Antioxidant enzymes had highest activities in mice treated with T. ramosissima extract and the level of lipid peroxides was the lowest. The tested extract had higher content of polyphenols comparative with V. myrtillus. Our results sustain the efficiency of T. ramosissima extracts on normalizing the effects of oxidative stress in diabetes.

scholarly journals Determination of carnosine, anserine, homocarnosine, pentosidine and thiobarbituric acid reactive substances contents in meat from different animal species

2011 ◽  
Vol 126 (4) ◽  
pp. 1939-1947 ◽  
Author(s):  
Pier Giorgio Peiretti ◽  
Claudio Medana ◽  
Sonja Visentin ◽  
Valeria Giancotti ◽  
Valentina Zunino ◽  
...  
Keyword(s):  
Animal Species ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances
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