An inexpensive procedure for the rapid determination of total nitrogen in multiple biological samples

1977 ◽  
Vol 28 (3) ◽  
pp. 312-316 ◽  
Author(s):  
Roger Sylvester-Bradley ◽  
Stephen G. Howitt
Keyword(s):  
Total Nitrogen ◽  
Biological Samples ◽  
Rapid Determination ◽  
Inexpensive Procedure

Rapid determination of glutamine in biological samples by high-performance liquid chromatography

1993 ◽  
Vol 218 (2) ◽  
pp. 159-168 ◽  
Author(s):  
Tom Teerlink ◽  
Marcel W.T. Hennekes ◽  
Paul A.M. van Leeuwen ◽  
Alexander Houdijk
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Biological Samples ◽  
High Performance ◽  
Rapid Determination

scholarly journals Rapid Determination of Lysine in Biological Samples by Isocratic Liquid Chromatography

1999 ◽  
Vol 82 (4) ◽  
pp. 809-813 ◽  
Author(s):  
Mamun M Or-Rashid ◽  
Ryoji Onodera ◽  
Shaila Wadud ◽  
Mohamed-Emad A Nasser ◽  
Mohammad R Amin
Keyword(s):  
Liquid Chromatography ◽  
Biological Samples ◽  
Limit Of Detection ◽  
Rapid Determination ◽  
Rumen Fluid ◽  
Uv Detector ◽  
Rumen Microorganisms ◽  
Microbial Nitrogen ◽  
Limit Of Quantitation

Abstract A simple, rapid, and sensitive method was developed for detection and quantitation of lysine (Lys) in various biological samples by isocratic liquid chromatography (LC). Samples containing Lys and other amino acids were derivatized with 9-fluorenylmethyl chloroformate (FMOC-CI). The mobile phase used for isocratic elution was 50 mmol/L sodium acetate buffer (pH 4.20)-acetonitrile (43 + 57, v/v). Lys was detected with a UV detector at 265 nm. The derivatized Lys eluted from a LiChrospher 100 RP-18 (150× 4.0 mm id) column at a retention time of 5.6 min. The limit of detection was 0.73 μmol/L (signal-to-noise [S/N] ratio, 3:1), and the limit of quantitation was 2.37 μmol/L (S/N ratio, 10:1). Lys recoveries from fortified biological samples were >97.5%. Average Lys contents found in rumen fluid samples collected before the morning feeding and at 2.0,4.0, and 6.0 h after feeding were 4.26,3.34,3.58, and 3.82 μmol/L, respectively. The hydrolysate of a sample of mixed rumen microorganisms collected before the morning feeding was determined to contain 1.372 μmol/mg microbial nitrogen in the form of Lys. The Lys concentrations of human plasma, goat plasma, human urine, and goat urine were 140.0, 102.0,58.0, and 32.0 μmol/L, respectively.

scholarly journals Rapid determination of quaternary protein structures in complex biological samples

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Simon Hauri ◽  
Hamed Khakzad ◽  
Lotta Happonen ◽  
Johan Teleman ◽  
Johan Malmström ◽  
...  
Keyword(s):  
Biological Samples ◽  
Rapid Determination ◽  
Protein Structures

Development of a magnetic dispersive micro-solid-phase extraction method based on a deep eutectic solvent as a carrier for the rapid determination of meloxicam in biological samples

2020 ◽  
Vol 12 (18) ◽  
pp. 2331-2337
Author(s):  
Samira Rastbood ◽  
Mohammad Reza Hadjmohammadi ◽  
Seyedeh Maedeh Majidi
Keyword(s):  
Solid Phase Extraction ◽  
Biological Samples ◽  
Extraction Method ◽  
Solid Phase ◽  
Rapid Determination ◽  
Deep Eutectic Solvent ◽  
Environmentally Friendly ◽  
Phase Extraction

An environmentally friendly magnetic dispersive micro solid phase extraction based on a deep eutectic solvent as a carrier and disperser of adsorbents.

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