The expressible fluid of fish fillets. XI.—ice crystal formation and cell damage in cod muscle frozen before rigor mortis

1961 ◽  
Vol 12 (6) ◽  
pp. 442-449 ◽  
Author(s):  
R. M. Love ◽  
S. B. Haraldsson
Keyword(s):  
Cell Damage ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Rigor Mortis ◽  
Fish Fillets ◽  
Ice Crystal Formation

scholarly journals FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

1972 ◽  
Vol 53 (1) ◽  
pp. 116-126 ◽  
Author(s):  
Helmut Plattner ◽  
Walter M. Fischer ◽  
Werner W. Schmitt ◽  
Luis Bachmann
Keyword(s):  
Cell Damage ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Unicellular Organisms ◽  
Freeze Etching ◽  
Ice Crystal Formation

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.

The “KIMWIPE” Effect in Ultra-Thin Cryosections

1985 ◽  
Vol 43 ◽  
pp. 458-459
Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Ice Crystals ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Thin Sections ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried ◽  
Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

scholarly journals Effects of freshness on thawing drip and ice crystal formation in frozen spotted mackerel Scomber australasicus

2015 ◽  
Vol 81 (1) ◽  
pp. 124-129 ◽  
Author(s):  
KANAKO HASHIMOTO ◽  
TOKIFUSA KAWASHIMA ◽  
NOBUYUKI YOSHINO ◽  
TAKAAKI SHIRAI ◽  
AKIHIDE TAKIGUCHI
Keyword(s):  
Crystal Formation ◽  
Ice Crystal ◽  
Ice Crystal Formation

Cryofixation of various biological samples by high pressure freezing

1992 ◽  
Vol 50 (1) ◽  
pp. 752-753
Author(s):  
LUCY RU-SIU YIN
Keyword(s):  
High Pressure ◽  
Biological Samples ◽  
High Rate ◽  
Crystal Formation ◽  
Chemical Fixation ◽  
Ice Crystal ◽  
High Pressure Freezing ◽  
Native State ◽  
Biological Specimen ◽  
Ice Crystal Formation

The ultimate aim of ultrastructural fixation of biological specimen is to preserve all the compartments in their native state. Cryofixation is a superior method than conventional chemical fixation in reaching this goal. However, ice crystal formation during cryofixation often damages the structures. High pressure (2100 bar) freezing provides a way to alter freezing properties while cool down the specimen at a relatively high rate, minimizing the ice crystal formation. Nearly vitrified samples(up to 500 um) have been obtained with this method. Samples in suspension tend to get lost during high pressure freezing. The low percentage (∼30%) of successfully cryofixed specimens can be improved if the sample completely fills the cavity of the metal specimen carriers in which the specimen is frozen. Various methods to overcome sample loss are reported in this study.

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