Direct separation and purification of α ‐lactalbumin from cow milk whey by aqueous two‐phase flotation of thermo‐sensitive polymer/phosphate

2021 ◽  
Author(s):  
Bin Jiang ◽  
Linlin Wang ◽  
Meichan Wang ◽  
Shuang Wu ◽  
Xiaojing Wang ◽  
...  
Keyword(s):  
Cow Milk ◽  
Separation And Purification ◽  
Two Phase ◽  
Milk Whey ◽  
Direct Separation

Separation and purification of horseradish peroxidase from horseradish roots using a novel integrated method

2021 ◽  
Author(s):  
Wei Ji ◽  
Wenmei Ao ◽  
Mengqiu Sun ◽  
Chunlai Feng ◽  
Yun Wang
Keyword(s):  
Horseradish Peroxidase ◽  
Phase Extraction ◽  
Separation And Purification ◽  
Two Phase ◽  
Affinity Precipitation ◽  
Integrated Method ◽  
Novel Method ◽  
Temperature Controlled ◽  
Aqueous Two Phase Extraction

The aim of the present work was to develop a novel method integrating two-step aqueous two-phase extraction and temperature-controlled affinity precipitation for the separation and purification horseradish peroxidase (HRP) from...

scholarly journals Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from Rhodococcus sp. in Aqueous/Organic Biphasic System

2015 ◽  
Vol 8s1 ◽  
pp. BCI.S21580 ◽  
Author(s):  
Ke Wu ◽  
Wei Li ◽  
Jianrui Song ◽  
Tao Li
Keyword(s):  
Cholesterol Oxidase ◽  
Enzymatic Reaction ◽  
Reaction System ◽  
Phase System ◽  
Separation And Purification ◽  
Two Phase ◽  
Whole Cells ◽  
Steroid Drugs ◽  
Set Up ◽  
The Cost

Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product.

scholarly journals Systematic Separation and Purification of Alkaloids from Euchresta tubulosa Dunn. by Various Chromatographic Methods

Processes ◽  
2019 ◽  
Vol 7 (12) ◽  
pp. 924
Author(s):  
Wei-Xin Li ◽  
Huan Wang ◽  
Ai-Wen Dong
Keyword(s):  
Natural Products ◽  
Silica Gel ◽  
Column Chromatography ◽  
High Speed ◽  
High Performance ◽  
Ionization Mass ◽  
Separation And Purification ◽  
Two Phase ◽  
Rapid Separation ◽  
Chemical Structures

High-speed countercurrent chromatography (HSCCC) and silica gel column chromatography were used to separate and purify alkaloids from Chinese herbal medicine Euchresta tubulosa Dunn. The purpose of this study is to provide a system mode for rapid separation of alkaloids from natural products. In the experiment, the eluent of silica gel column chromatography was screened by thin layer chromatography (TLC) to obtain four components with different polarity. Then, the two-phase solvent systems of different components were selected and purified by HSCCC. Four alkaloids with relatively high content were obtained by this mode successfully, including matrine (28 mg), oxymatrine (32 mg), N-formyl cytisine (24 mg), and cytisine (58 mg). The purity was higher than 91% by high performance liquid chromatography–ultraviolet (HPLC-UV) and their chemical structures were identified by nuclear magnetic resonance (NMR) and electron ionization mass spectrometry (EI-MS). The results showed that the combination of HSCCC and silica gel column chromatography could make alkaloids from natural products separate systematically.

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