Caffeic acid modulates methane production and rumen fermentation in an opposite way with high‐forage or high‐concentrate substrate in vitro

2020 ◽  
Author(s):  
Qing Jin ◽  
Wei You ◽  
Xiuwen Tan ◽  
Guifen Liu ◽  
Xianglun Zhang ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Methane Production ◽  
Rumen Fermentation ◽  
High Forage

scholarly journals Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Avijit Dey ◽  
Shyam Sundar Paul ◽  
Puran Chand Lailer ◽  
Satbir Singh Dahiya
Keyword(s):  
Environmental Pollution ◽  
Methane Production ◽  
Rumen Fluid ◽  
Bubalus Bubalis ◽  
Rumen Fermentation ◽  
Acetyl Esterase ◽  
Garlic Oil ◽  
Enteric Methane ◽  
In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

scholarly journals Effect of essential oils and aqueous extracts of plants on in vitro rumen fermentation and methane production

2022 ◽  
Vol 10 (1) ◽  
pp. 1-9
Author(s):  
Aarón A. Molho-Ortiz ◽  
Atmir Romero-Pérez ◽  
Efrén Ramírez-Bribiesca ◽  
Claudia C. Márquez-Mota ◽  
Francisco A. Castrejón-Pineda ◽  
...  
Keyword(s):  
Essential Oils ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Aqueous Extracts ◽  
In Vitro Rumen Fermentation

scholarly journals Effects of Geraniol and Camphene on in Vitro Rumen Fermentation and Methane Production

2017 ◽  
Vol 48 (2) ◽  
pp. 63-69
Author(s):  
M. Joch ◽  
V. Kudrna ◽  
B. Hučko
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Positive Control ◽  
Rumen Fermentation ◽  
Dry Matter Digestibility

AbstractThe objective of this study was to determine the effects of geraniol and camphene at three dosages (300, 600, and 900 mg l-1) on rumen microbial fermentation and methane emission in in vitro batch culture of rumen fluid supplied with a 60 : 40 forage : concentrate substrate (16.2% crude protein, 33.1% neutral detergent fibre). The ionophore antibiotic monensin (8 mg/l) was used as positive control. Compared to control, geraniol significantly (P < 0.05) reduced methane production with increasing doses, with reductions by 10.2, 66.9, and 97.9%. However, total volatile fatty acids (VFA) production and in vitro dry matter digestibility were also reduced (P < 0.05) by all doses of geraniol. Camphene demonstrated weak and unpromising effects on rumen fermentation. Camphene did not decrease (P > 0.05) methane production and slightly decreased (P < 0.05) VFA production. Due to the strong antimethanogenic effect of geraniol a careful selection of dose and combination with other antimethanogenic compounds may be effective in mitigating methane emission from ruminants. However, if a reduction in total VFA production and dry matter digestibility persisted in vivo, geraniol would have a negative effect on animal productivity.

scholarly journals Use of blanks to determine in vitro net gas and methane production when using rumen fermentation modifiers

2011 ◽  
Vol 166-167 ◽  
pp. 155-162 ◽  
Author(s):  
R.C. Araujo ◽  
A.V. Pires ◽  
G.B. Mourão ◽  
A.L. Abdalla ◽  
S.M.A. Sallam
Keyword(s):  
Methane Production ◽  
Rumen Fermentation

scholarly journals Effect of Lactobacillus mucosae on In vitro Rumen Fermentation Characteristics of Dried Brewers Grain, Methane Production and Bacterial Diversity

2014 ◽  
Vol 27 (11) ◽  
pp. 1562-1570 ◽  
Author(s):  
Alvin P. Soriano ◽  
Lovelia L. Mamuad ◽  
Seon-Ho Kim ◽  
Yeon Jae Choi ◽  
Chang Dae Jeong ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Methane Production ◽  
Rumen Fermentation ◽  
Lactobacillus Mucosae

scholarly journals The effect of a high forage diet and different fat sources on rumen fermentation in vitro

2006 ◽  
Vol 15 (Suppl. 1) ◽  
pp. 133-136
Author(s):  
D. Jalč ◽  
A. Potkański ◽  
M. Szumacher-Strabel ◽  
J. Kowalczyk ◽  
A. Cieślak
Keyword(s):  
Rumen Fermentation ◽  
High Forage

scholarly journals Effect of cashew nut shell liquid at varying inclusion levels on rumen fermentation and methane production in vitro

2020 ◽  
Vol 8 (2) ◽  
pp. 82-87
Author(s):  
Adeola Patience Adetunji ◽  
Ronke Yemisi Aderinboye ◽  
Kafayat Omowunmi Adebayo ◽  
Victoria Olubunmi Ojo ◽  
Peter Ayodeji Idowu ◽  
...  
Keyword(s):  
Methane Production ◽  
Rumen Fermentation ◽  
Cashew Nut Shell Liquid ◽  
Cashew Nut ◽  
Cashew Nut Shell

Effect of 9,10-Anthraquinone on rumen methane production as studied in vitro and in vivo

2003 ◽  
Vol 2003 ◽  
pp. 122-122
Author(s):  
V. Fievez ◽  
B. Vlaeminck ◽  
W. Steinberg ◽  
I. Immig ◽  
D. Demeyer
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Rumen Fermentation ◽  
Dose Effect ◽  
Total Volatile ◽  
Total Volatile Fatty Acid

In vitro supplementation of 0.05% [on a substrate basis (wt/wt)] - but not of 0.01% - of 9,10-Anthraquinone (AQ) inhibited rumen methanogenesis, reduced total volatile fatty acid (VFA) concentrations and molar proportions of acetate (Acet), increased proportions of propionate (Prop) and butyrate (But) and resulted sometimes in H2 accumulation (Garcia-Lopez et al., 1996). In vivo administration of high amounts of AQ [5% on a substrate basis (wt/wt)] to lambs depressed CH4 and increased H2 concentrations in ruminal gases during the complete 19 days of administration, whereas original concentrations were re-installed within 6 days after the removal of AQ from the diet (Kung et al., 1996). In this experiment we aimed to study the dose effect of AQ on in vitro rumen fermentation and modifications to rumen fermentation when administering 0.05% of AQ in vivo.

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