scholarly journals Protein, weight, and oil prediction by single‐seed near‐infrared spectroscopy for selection of seed quality and yield traits in pea ( Pisum sativum )

2020 ◽  
Vol 100 (8) ◽  
pp. 3488-3497 ◽  
Author(s):  
Gokhan Hacisalihoglu ◽  
Jelani Freeman ◽  
Paul R. Armstrong ◽  
Brad W Seabourn ◽  
Lyndon D Porter ◽  
...  
2020 ◽  
Author(s):  
Gokhan Hacisalihoglu ◽  
Jelani Freeman ◽  
Paul R. Armstrong ◽  
Brad W. Seabourn ◽  
Lyndon D. Porter ◽  
...  

Abstract Background: Pea (Pisum sativum) is a prevalent cool season crop that produces seeds valued for high protein content. Modern cultivars have incorporated several traits that improved harvested yield. However, progress toward improving seed quality has received less emphasis, in part due to the lack of tools for easily and rapidly measuring seed traits. In this study we evaluated the accuracy of single-seed near-infrared spectroscopy (NIRS) for measuring pea seed weight, protein, and oil content. A total of 96 diverse pea accessions were analyzed using both single-seed NIRS and wet chemistry methods. To demonstrate field relevance, the single-seed NIRS protein prediction model was used to determine the impact of seed treatments and foliar fungicides on protein content of harvested dry peas in a field trial. Results: External validation of Partial Least Squares (PLS) regression models showed high prediction accuracy for protein and weight (R2 = 0.94 for both) and less accuracy for oil (R2 = 0.75). Single seed weight was not significantly correlated with protein or oil content in contrast to previous reports. In the field study, the single-seed NIRS predicted protein values were within 1% of an independent analytical reference measurement and were sufficiently precise to detect small treatment effects. Conclusion: The high accuracy of protein and weight estimation show that single-seed NIRS could be used in the dual selection of high protein, high weight peas early in the breeding cycle allowing for faster genetic advancement toward improved pea nutritional quality.


2017 ◽  
Vol 10 (2) ◽  
pp. 506-514 ◽  
Author(s):  
A Sandak ◽  
J Sandak ◽  
B Waliszewska ◽  
M Zborowska ◽  
M Mleczek

Talanta ◽  
2013 ◽  
Vol 112 ◽  
pp. 136-142 ◽  
Author(s):  
Guiyun Wang ◽  
Mingyu Ma ◽  
Zhuoyong Zhang ◽  
Yuhong Xiang ◽  
Peter de B. Harrington

Silva Fennica ◽  
2015 ◽  
Vol 49 (5) ◽  
Author(s):  
Abolfazl Daneshvar ◽  
Mulualem Tigabu ◽  
Asaddollah Karimidoost ◽  
Per Oden

1989 ◽  
Vol 43 (2) ◽  
pp. 328-335 ◽  
Author(s):  
Tormod Næs ◽  
Tomas Isaksson

General principles for the selection of samples in the calibration of near-infrared (NIR) spectrophotometers are discussed. The discussion ends with suggestions of alternative strategies, which are illustrated by examples.


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