scholarly journals Selection of tomato landraces with high fruit yield and nutritional quality under elevated temperatures

2020 ◽  
Vol 100 (6) ◽  
pp. 2791-2799 ◽  
Author(s):  
Aurelia Scarano ◽  
Fabrizio Olivieri ◽  
Carmela Gerardi ◽  
Marina Liso ◽  
Maurizio Chiesa ◽  
...  
2022 ◽  
Vol 291 ◽  
pp. 110559
Author(s):  
Santino Seabra Junior ◽  
Jéssica Gawski Casagrande ◽  
Claudia Aparecida de Lima Toledo ◽  
Franciely da Silva Ponce ◽  
Fernanda da Silva Ferreira ◽  
...  

2021 ◽  
Vol 20 (3) ◽  
pp. 325-339
Author(s):  
Frederick Witfeld ◽  
Dominik Begerow ◽  
Marco Alexandre Guerreiro

AbstractThermophilic, thermotolerant and heat-resistant fungi developed different physiological traits, enabling them to sustain or even flourish under elevated temperatures, which are life-hostile for most other eukaryotes. With the growing demand of heat-stable molecules in biotechnology and industry, the awareness of heat-adapted fungi as a promising source of respective enzymes and biomolecules is still increasing. The aim of this study was to test two different strategies for the efficient isolation and identification of distinctly heat-adapted fungi from easily accessible substrates and locations. Eight compost piles and ten soil sites were sampled in combination with different culture-dependent approaches to describe suitable strategies for the isolation and selection of thermophilous fungi. Additionally, an approach with a heat-shock treatment, but without elevated temperature incubation led to the isolation of heat-resistant mesophilic species. The cultures were identified based on morphology, DNA barcodes, and microsatellite fingerprinting. In total, 191 obtained isolates were assigned to 31 fungal species, from which half are truly thermophilic or thermotolerant, while the other half are heat-resistant fungi. A numerous amount of heat-adapted fungi was isolated from both compost and soil samples, indicating the suitability of the used approaches and that the richness and availability of those organisms in such environments are substantially high.


2014 ◽  
Vol 10 ◽  
pp. 2858-2873 ◽  
Author(s):  
Robert Francke

Due to the fact that the major portion of pharmaceuticals and agrochemicals contains heterocyclic units and since the overall number of commercially used heterocyclic compounds is steadily growing, heterocyclic chemistry remains in the focus of the synthetic community. Enormous efforts have been made in the last decades in order to render the production of such compounds more selective and efficient. However, most of the conventional methods for the construction of heterocyclic cores still involve the use of strong acids or bases, the operation at elevated temperatures and/or the use of expensive catalysts and reagents. In this regard, electrosynthesis can provide a milder and more environmentally benign alternative. In fact, numerous examples for the electrochemical construction of heterocycles have been reported in recent years. These cases demonstrate that ring formation can be achieved efficiently under ambient conditions without the use of additional reagents. In order to account for the recent developments in this field, a selection of representative reactions is presented and discussed in this review.


2021 ◽  
Vol 108 (december) ◽  
Author(s):  
Gurve VR ◽  
◽  
Swarna Priya R ◽  
Pugalendhi L ◽  
Karthikeyan G ◽  
...  

Okra is India’s most widely grown tropical and subtropical vegetable crop. Despite widespread cultivation, crop yield and productivity remain subpar. Yellow vein mosaic virus (YVMV) disease is a serious threat to Indian okra production, causing yield losses ranging from 17.09 to 96.49 per cent. In this context, the present study was aimed to assess the variability and character association of 74 elite germplasm accessions for thirteen quantitative and quality traits. High phenotypic and genotypic coefficient of variation (PCV and GCV) were observed among all evaluated traits except fruit diameter, which showed a moderate PCV and GCV. All traits had high heritability and high genetic advance, implying that the heritability was most likely due to additive gene effects. Direct selection of these traits would be advantageous for okra improvement, especially in developing a YVMV resistant genotype. Fruit yield per plant exhibited a significant and positive genotypic as well as phenotypic correlation with fruit weight, fruit length, peroxidase activity, polyphenol activity, fruit diameter, total phenolic content and plant height, whereas node at which the first flower appeared and YVMV were negatively correlated to fruit yield per plant. Correlation studies indicated YVMV resistance towards high total phenolic content, polyphenol oxidase, and peroxidase. The high positive direct effects on fruit yield per plant were observed for fruit weight followed by number of fruit per plant and total phenolic content. This study revealed that simultaneous selection of these traits can help in increasing yield and YVMV resistance.


2021 ◽  
Author(s):  
Steve Ingistov ◽  
Douglas Nagy

Abstract Turbine wheels are critical elements and the integrity of their forgings is extremely important. The procurement of wheel forgings utilized oversized outside diameters in order to provide ample amount of material from which test material was cut out. Test specimens were produced in accordance with relevant ASTM standards. Test specimens were divided into three groups; tension, impact and hardness / metallography. Tension and impact tests at sub-zero, room and elevated temperatures were conducted in presence of owner’s inspectors at an independent certified laboratory. Once all specimens passed the tests, the semi-machined forgings were released to the machining facility. Machined forgings were then sent for over-speed tests at sub-zero and elevated temperatures. Over-speed testing under sub-zero temperature was the ultimate test of the forgings. Over-speed testing of the forgings under elevated temperatures served to minimize residual tensile stresses at the bore of the wheel and convert them to beneficial compressive stresses. The above described tests of turbine Elements forging are critical when the owner selects third party producers to reverse engineer and manufacture these elements. This paper details the selection of the tests, the execution of the owner’s acceptance testing program, especially the over-speed tests, and how this helps to ensure the high integrity of critical rotating elements for a mid-size heavy industrial frame gas turbine.


2001 ◽  
Vol 10 (2) ◽  
pp. 096369350101000 ◽  
Author(s):  
J. George ◽  
E.T.J. Klompen ◽  
T. Peijs

Over the last couple of years, an increasing amount of research has been dedicated to the use of flax fibres as an environmentally friendly alternative to glass fibres in engineering composites. Because of the relatively poor thermal stability of flax fibres compared to glass fibres, knowledge of the thermal degradation profile of these natural fibres is essential for the selection of potential matrix candidates and/or the development of feasible processing and recycling routes. Especially in combination with thermoplastic resins, composite (re)processing may have a detrimental effect on the mechanical properties of the natural fibres. This study reports on the effect of the exposure of two types of flax fibres, green flax and a new upgraded flax fibre called Duralin, to elevated temperatures using thermogravimetric analysis (TGA), residual strength measurements and environmental scanning electron microscopy (ESEM) studies.


2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Hao-Chun Chiang ◽  
Rastislav Levicky

The ability of different crosslinkers to crosslink nanometer thick films of the polymer poly(mercaptopropyl)methylsiloxane (PMPMS), thus stabilizing these films on solid supports, was investigated. The four crosslinkers included 1,11-bismaleimidotriethyleneglycol (BM(PEG)3), tris-(2-maleimidoethyl)amine (TMEA), bismaleimidohexane (BMH), and 1,1′-(methylenedi-4,1-phenylene) bismaleimide (BMDPM). PMPMS films treated with the four crosslinkers were compared in the effectiveness of achieved crosslinking, continuity and stability of the films to rearrangement at elevated temperatures, and modification with single-stranded DNA. The results of electrochemical analyses show that more hydrophilic crosslinkers had difficulty reacting fully with PMPMS thiols, even in these nanometer thin layers. This observation highlights the critical importance of selecting crosslinkers that are chemically compatible. Optimal selection of crosslinker yielded films in which the polymer film was largely incapable of rearranging, even at elevated temperatures, yielding reproducible and stable layers. These results validate use of these supports for applications such as monitoring thermal denaturation of immobilized DNA duplexes.


2012 ◽  
Vol 75 (10) ◽  
pp. 1743-1750 ◽  
Author(s):  
ANDREA KROJ ◽  
HERBERT SCHMIDT

Enterohemorrhagic Escherichia coli O157:H7 strains are important foodborne pathogens that are often transmitted to humans by the ingestion of raw or undercooked meat of bovine origin. To investigate adaptation of this pathogen during persistence and growth in ground meat, we established an in vivo expression technology model to identify genes that are expressed during growth in this food matrix under elevated temperatures (42°C). To improve on the antibiotic-based selection method, we constructed the promoter trap vector pAK-1, containing a promoterless kanamycin resistance gene. A genomic library of E. coli O157:H7 strain EDL933 was constructed in pAK-1 and used for promoter selection in ground meat. The 20 in vivo expressed genes identified were associated with transport processes, metabolism, macromolecule synthesis, and stress response. For most of the identified genes, only hypothetical functions could be assigned. The results of our study provide the first insights into the complex response of E. coli O157:H7 to a ground meat environment under elevated temperatures and establish a suitable vector for promoter studies or selection of in vivo induced promoters in foods such as ground meat.


2020 ◽  
Vol 48 (4) ◽  
pp. 2198-2214
Author(s):  
Santina R. SANTANA ◽  
Derly J. H. Da SILVA ◽  
Leonardo L. BHERING ◽  
Ronaldo S. GOMES ◽  
Renato D. S. ROSADO ◽  
...  

Fruits of Capsicum chinense, a native species of Amazon Basin, express high levels of bioactive components such as vitamin C and carotenoids; some of them with pronounced pro-vitamin A activity such as β-carotene, which confers high economic potential to this species. The characterization of C. chinense germplasm helps in its management and conservation. Therefore, this practice is considered crucial for the identification of genotypes with superior characteristics, especially in relation to agronomic aspects and chemical-nutritional characteristics of fruits. This study aimed to characterize 55 C. chinense accessions collected from the Brazilian Amazon in terms of their agronomic and chemical-nutritional descriptors aiming to identify superior genotypes for these traits. The characterization was performed in a completely randomized design with 5 replications in non-heated environment. There was significant difference for all descriptors, confirming the variability among accessions. High heritability estimates for descriptors, such as fruit yield (95.1%) and vitamin C content of fruit (92.4%), was found to be associated with high CVg/CVe ratios of these traits, indicating a favorable condition for the selection of superior genotypes for these characteristics. A considerable part of the accessions expressed averages higher than the checks, notably for the fruit yield and the content of vitamin C. The cluster analysis resulted in the formation of 11 groups, corroborating the high variability of accessions for the agronomic and chemical-nutritional aspects of fruits. The evaluated C. chinense germplasm thus expressed high fruit yield and vitamin C content in the fruits, which makes it a promising source for the selection of superior genotypes.


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