CW measurements of resonance Raman profiles, line-widths, and cross-sections of fluorescent dyes: application to Nile Blue A in water and ethanol

2013 ◽  
Vol 44 (4) ◽  
pp. 573-581 ◽  
Author(s):  
Antoine Reigue ◽  
Baptiste Auguié ◽  
Pablo G. Etchegoin ◽  
Eric C. Le Ru
Keyword(s):  
Cross Sections ◽  
Fluorescent Dyes ◽  
Nile Blue ◽  
Resonance Raman ◽  
Nile Blue A ◽  
Line Widths

scholarly journals Evidence for Dual Site Binding of Nile Blue A toward DNA: Spectroscopic, Thermodynamic, and Molecular Modeling Studies

ACS Omega ◽  
2021 ◽  
Vol 6 (4) ◽  
pp. 2613-2625
Author(s):  
Mukti Mohammad ◽  
Harun Al Rasid Gazi ◽  
Kumud Pandav ◽  
Prateek Pandya ◽  
Md. Maidul Islam
Keyword(s):  
Molecular Modeling ◽  
Nile Blue ◽  
Nile Blue A ◽  
Modeling Studies ◽  
Molecular Modeling Studies ◽  
Dual Site ◽  
Site Binding

Novel 2,1,3-Benzothiadiazole-Based Red-Fluorescent Dyes with Enhanced Two-Photon Absorption Cross-Sections

2006 ◽  
Vol 12 (8) ◽  
pp. 2303-2317 ◽  
Author(s):  
Shin-ichiro Kato ◽  
Taisuke Matsumoto ◽  
Motoyuki Shigeiwa ◽  
Hideki Gorohmaru ◽  
Shuuichi Maeda ◽  
...  
Keyword(s):  
Cross Sections ◽  
Fluorescent Dyes ◽  
Photon Absorption ◽  
Absorption Cross ◽  
Two Photon Absorption ◽  
Two Photon

On Colouring Epon-Embedded Tissue Sections with Sudan Black B or Nile Blue A for Light Microscopy

1963 ◽  
Vol s3-104 (65) ◽  
pp. 109-115
Author(s):  
S. M. McGEE-RUSSELL ◽  
N. B. SMALE
Keyword(s):  
Lipid Droplets ◽  
Nile Blue ◽  
Thin Sections ◽  
Nile Blue A ◽  
Sudan Black B ◽  
Glass Slides ◽  
Acetone Water ◽  
Negative Effect ◽  
Blue Background ◽  
Dark Blue

Sections of osmium-fixed tissues embedded in epon 812 colour with either Sudan black B or Nile blue A solutions to reveal a variety of detail by direct microscopy with normal apochromatic or semi-apochromatic objectives. The clarity of the coloration gives a picture fully comparable to that seen by phase-contrast microscopy. The plastic is not removed, 1-µ sections or thinner sections down to green or gold, are mounted on clean glass slides by drying down from 20% acetone/water after flattening on a hot plate. Colouring is carried out at room temperature in Sudan black B (saturated solution in 70% alcohol) for 1 to 2 h. The result is a reversed or negative effect, for the epon plastic takes the stain avidly, but dense elements of the tissue do not, and appear white against a blue background of stained plastic. Lipid droplets retain a capacity to colour, becoming dark blue to blue-black. Nile blue sulphate ( 1% aqueous solution colours thin sections of tissue in 1 to 2 h at 60° C, acting apparently as a basic dye on most cell elements, and also colours lipid droplets dark blue. After both techniques the sections are mounted in Farrants's medium.

Rapid quantification of polyhydroxyalkanoates (PHA) concentration in activated sludge with the fluorescent dye Nile blue A

2011 ◽  
Vol 64 (3) ◽  
pp. 747-753 ◽  
Author(s):  
M. Oshiki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Activated Sludge ◽  
Wastewater Treatment Plants ◽  
Nile Blue ◽  
Maximum Level ◽  
Fluorescence Measurement ◽  
Batch Reactors ◽  
Floc Size ◽  
Nile Blue A ◽  
On Line ◽  
Rapid Quantification

The present study was conducted (1) to develop a rapid quantification method of polyhydroxyalkanoates (PHA) concentration in activated sludge by Nile blue A staining and fluorescence measurement and (2) to perform on-line monitoring of PHA concentrations in activated sludge. Activated sludge samples collected from laboratory scale sequencing batch reactors and full-scale wastewater treatment plants were stained with Nile blue A and their fluorescence intensities were determined. There was a high correlation (R2 > 0.97) between the fluorescence intensities of Nile blue A and PHA concentrations in activated sludge determined by gas chromatography. The Nile blue A staining and fluorescence measurement method allows us to determine PHA concentrations in activated sludge within only five minutes and up to 96 samples can be measured at once by using microplate reader. On-line monitoring of PHA concentrations in activated sludge was achieved by using a fluorometer equipped with a flow cell and the time point at which PHA concentration in activated sludge reached the maximum level could be identified. In addition, we examined the influence of pH, floc size and co-existing chemicals in activated sludge suspension on the fluorescence intensities of Nile blue A.

Kinetic determination of trace levels of selenium(IV) and total selenium by Nile Blue A/hydrogen peroxide method

1998 ◽  
Vol 128 (1-2) ◽  
pp. 43-48 ◽  
Author(s):  
Gordana A. Milovanović ◽  
Radivoj B. Petronijević ◽  
Mira M. Čakar
Keyword(s):  
Hydrogen Peroxide ◽  
Nile Blue ◽  
Kinetic Determination ◽  
Nile Blue A ◽  
Total Selenium ◽  
Peroxide Method
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