Surface-enhanced Raman spectroscopy for quantitative measurement of lactic acid at physiological concentration in human serum

2010 ◽  
Vol 41 (12) ◽  
pp. 1610-1614 ◽  
Author(s):  
Po-Hsiang Hsu ◽  
Huihua Kenny Chiang
Keyword(s):  
Raman Spectroscopy ◽  
Lactic Acid ◽  
Human Serum ◽  
Quantitative Measurement ◽  
Surface Enhanced Raman Spectroscopy ◽  
Physiological Concentration ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Detection of pesticide residue distribution on fruit surfaces using surface-enhanced Raman spectroscopy imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 4726-4730 ◽  
Author(s):  
Jiannan Chen ◽  
Daming Dong ◽  
Song Ye
Keyword(s):  
Raman Spectroscopy ◽  
Pesticide Residue ◽  
Pesticide Residues ◽  
Quantitative Measurement ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface-enhanced Raman spectroscopy (SERS) is an emerging technique for the detection of pesticide residues on food surfaces, permitting quantitative measurement of pesticide residues without pretreating the sample.

Combination assay of lung cancer associated serum markers using surface-enhanced Raman spectroscopy

2016 ◽  
Vol 4 (10) ◽  
pp. 1811-1817 ◽  
Author(s):  
Chunyuan Song ◽  
Yanjun Yang ◽  
Boyue Yang ◽  
Linghua Min ◽  
Lianhui Wang
Keyword(s):  
Lung Cancer ◽  
Raman Spectroscopy ◽  
Human Serum ◽  
Surface Enhanced Raman Spectroscopy ◽  
Serum Markers ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

A highly sensitive and specific SERS-based combination assay of lung cancer associated serum markers is reported, and the LODs of CEA and NSE in human serum specimens are 1.48 pg mL−1 and 2.04 pg mL−1, respectively.

scholarly journals Antibody-Free Discrimination of Protein Biomarkers in Human Serum Based on Surface-Enhanced Raman Spectroscopy

2018 ◽  
Vol 90 (21) ◽  
pp. 12342-12346 ◽  
Author(s):  
Hao Ma ◽  
Xiaoying Sun ◽  
Lei Chen ◽  
Xiao Xia Han ◽  
Bing Zhao ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Human Serum ◽  
Surface Enhanced Raman Spectroscopy ◽  
Protein Biomarkers ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

scholarly journals Optical tweezers-controlled hotspot for sensitive and reproducible surface-enhanced Raman spectroscopy characterization of native protein structures

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xin Dai ◽  
Wenhao Fu ◽  
Huanyu Chi ◽  
Vince St. Dollente Mesias ◽  
Hongni Zhu ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Optical Tweezers ◽  
Single Molecule ◽  
Protein Structures ◽  
Surface Enhanced Raman Spectroscopy ◽  
Alpha Synuclein ◽  
Ensemble Averaging ◽  
Physiological Concentration ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

AbstractSurface-enhanced Raman spectroscopy (SERS) has emerged as a powerful tool to detect biomolecules in aqueous environments. However, it is challenging to identify protein structures at low concentrations, especially for the proteins existing in an equilibrium mixture of various conformations. Here, we develop an in situ optical tweezers-coupled Raman spectroscopy to visualize and control the hotspot between two Ag nanoparticle-coated silica beads, generating tunable and reproducible SERS enhancements with single-molecule level sensitivity. This dynamic SERS detection window is placed in a microfluidic flow chamber to detect the passing-by proteins, which precisely characterizes the structures of three globular proteins without perturbation to their native states. Moreover, it directly identifies the structural features of the transient species of alpha-synuclein among its predominant monomers at physiological concentration of 1 μM by reducing the ensemble averaging. Hence, this SERS platform holds the promise to resolve the structural details of dynamic, heterogeneous, and complex biological systems.

Sign in / Sign up

Export Citation Format

Share Document