Investigation into the protein composition of human tear fluid using centrifugal filters and drop coating deposition Raman spectroscopy

2009 ◽  
Vol 40 (2) ◽  
pp. 218-224 ◽  
Author(s):  
Jacob Filik ◽  
Nicholas Stone
Keyword(s):  
Raman Spectroscopy ◽  
Protein Composition ◽  
Tear Fluid ◽  
Coating Deposition

scholarly journals Analysis of protein composition and protein expression in the tear fluid of patients with congenital aniridia

2013 ◽  
Vol 94 ◽  
pp. 78-88 ◽  
Author(s):  
Robert Ihnatko ◽  
Ulla Edén ◽  
Neil Lagali ◽  
Anette Dellby ◽  
Per Fagerholm
Keyword(s):  
Protein Expression ◽  
Protein Composition ◽  
Tear Fluid ◽  
Congenital Aniridia

Drop-coating deposition Raman spectroscopy of liposomes

2011 ◽  
Vol 42 (8) ◽  
pp. 1606-1610 ◽  
Author(s):  
Eva Kočišová ◽  
Marek Procházka
Keyword(s):  
Raman Spectroscopy ◽  
Coating Deposition

scholarly journals Interaction of porphyrin/oligonucleotide complex with liposomes studied by drop coating deposition Raman spectroscopy

2010 ◽  
Vol 24 (3-4) ◽  
pp. 197-200 ◽  
Author(s):  
E. Kocišová ◽  
M. Procházka ◽  
J. Štepánek ◽  
P. Mojzeš
Keyword(s):  
Raman Spectroscopy ◽  
Lipid Composition ◽  
Hydrophobic Surface ◽  
Small Species ◽  
Drying Process ◽  
High Quality ◽  
Coating Deposition ◽  
Solution Sample ◽  
Plasmatic Membrane ◽  
Edge Part

Drop coating deposition Raman (DCDR) microspectroscopy was used to investigate interaction of the complexed cationic copper 5,10,15,20-tetrakis(1-methyl-4-pyridyl) porphyrin (CuP) and phosphorothioate analog of dT15oligonucleotide with liposomes, the lipid composition of which imitated the natural plasmatic membrane. Great advantage of dried drops on DCDR plates over a solution sample is that the specific drying process on the special hydrophobic surface efficiently separates liposomes from small species in the solvent. In our case, liposomes with bound CuP/oligonucleotide complexes formed a ring at the edge part of the dried drop while dried solution of this complex remained inside this ring. High quality spectra measured from the ring by using Raman confocal microspectrometer revealed unperturbed arrangement of lipid chains by the drying process, partial binding of the CuP/oligonucleotide complexes to liposomes, and a certain reorientation of lipid chains as a consequence of this interaction.

Paper-based surfaced enhanced Raman spectroscopy for drug level testing with tear fluid

2015 ◽  
Author(s):  
Kenji Yamada ◽  
Moe Yokoyama ◽  
Hieyong Jeong ◽  
Michiko Kido ◽  
Yuko Ohno
Keyword(s):  
Raman Spectroscopy ◽  
Drug Level ◽  
Tear Fluid

Drop coating deposition Raman spectroscopy of dipicolinic acid

2018 ◽  
Vol 49 (12) ◽  
pp. 2050-2052 ◽  
Author(s):  
Eva Kočišová ◽  
Marek Procházka
Keyword(s):  
Raman Spectroscopy ◽  
Dipicolinic Acid ◽  
Coating Deposition

scholarly journals Drop Coating Deposition Raman Spectroscopy of Fluorescein Isothiocyanate Labeled Protein

2010 ◽  
Vol 64 (10) ◽  
pp. 1078-1085 ◽  
Author(s):  
Dongmao Zhang ◽  
Karthikeshwar Vangala ◽  
Dongping Jiang ◽  
Sige Zou ◽  
Tibor Pechan
Keyword(s):  
Raman Spectroscopy ◽  
Fluorescein Isothiocyanate ◽  
Coating Deposition
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