Surface-enhanced Raman probing of biomolecules using Ag-coated porous glass-ceramic substrates

2005 ◽  
Vol 36 (11) ◽  
pp. 1082-1087 ◽  
Author(s):  
Z. Pan ◽  
S. H. Morgan ◽  
A. Ueda ◽  
R. Mu ◽  
Y. Cui ◽  
...  
Keyword(s):  
Porous Glass ◽  
Glass Ceramic ◽  
Ceramic Substrates ◽  
Surface Enhanced ◽  
Surface Enhanced Raman

Surface-Enhanced Raman Spectroscopy Using Silver-Coated Porous Glass-Ceramic Substrates

2005 ◽  
Vol 59 (6) ◽  
pp. 782-786 ◽  
Author(s):  
Z. Pan ◽  
A. Zavalin ◽  
A. Ueda ◽  
M. Guo ◽  
M. Groza ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Porous Glass ◽  
Glass Ceramic ◽  
Isonicotinic Acid ◽  
Rf Sputtering ◽  
Surface Enhanced Raman Scattering ◽  
Surface Enhanced Raman Spectroscopy ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering

Surface-enhanced Raman scattering (SERS) has been studied using a silver-coated porous glass-ceramic material as a new type of substrate. The porous glass-ceramic is in the CaO–TiO2–P2O5 system prepared by controlled crystallization and subsequent chemical leaching of the dense glass-ceramic, leaving a solid skeleton with pores ranging in size from 50 nm to submicrometer. Silver was coated on the surface of the porous glass-ceramic by radio frequency (RF) sputtering or e-beam evaporation in vacuum. SERS spectra of excellent quality were obtained from several dyes and carboxylic acid molecules, including rhodamine 6G, crystal violet, isonicotinic acid, and benzoic acid, using this new substrate. This new substrate showed a good compatibility with these molecules. The porous glass-ceramic with a nanometer-structured surface accommodated both test molecules and silver film. The absorbed molecules were therefore better interfaced with silver for surface-enhanced Raman scattering.

Bacterial detection and differentiation via direct volatile organic compound sensing with surface enhanced Raman spectroscopy

2017 ◽  
Author(s):  
Caitlin S. DeJong ◽  
David I. Wang ◽  
Aleksandr Polyakov ◽  
Anita Rogacs ◽  
Steven J. Simske ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Bacterial Infections ◽  
Direct Detection ◽  
Point Of Care ◽  
Surface Enhanced Raman Spectroscopy ◽  
E Coli ◽  
Recent Emergence ◽  
Surface Enhanced ◽  
Volatile Organic ◽  
Surface Enhanced Raman

Through the direct detection of bacterial volatile organic compounds (VOCs), via surface enhanced Raman spectroscopy (SERS), we report here a reconfigurable assay for the identification and monitoring of bacteria. We demonstrate differentiation between highly clinically relevant organisms: <i>Escherichia coli</i>, <i>Enterobacter cloacae</i>, and <i>Serratia marcescens</i>. This is the first differentiation of bacteria via SERS of bacterial VOC signatures. The assay also detected as few as 10 CFU/ml of <i>E. coli</i> in under 12 hrs, and detected <i>E. coli</i> from whole human blood and human urine in 16 hrs at clinically relevant concentrations of 10<sup>3</sup> CFU/ml and 10<sup>4</sup> CFU/ml, respectively. In addition, the recent emergence of portable Raman spectrometers uniquely allows SERS to bring VOC detection to point-of-care settings for diagnosing bacterial infections.

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