Resonance Raman and absorption characterization of cationic Co(II)-porphyrin in its complexes with nucleic acids: binding modes, nucleic base specificity and role of water in Co(II) oxidation processes

2005 ◽  
Vol 36 (10) ◽  
pp. 962-973 ◽  
Author(s):  
Sergei N. Terekhov ◽  
Victor A. Galievsky ◽  
Vladimir S. Chirvony ◽  
Pierre-Yves Turpin
Keyword(s):  
Nucleic Acids ◽  
Resonance Raman ◽  
Binding Modes ◽  
Oxidation Processes ◽  
Base Specificity

scholarly journals A unique peptide deformylase platform to rationally design and challenge novel active compounds

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Sonia Fieulaine ◽  
Rodolphe Alves de Sousa ◽  
Laure Maigre ◽  
Karim Hamiche ◽  
Mickael Alimi ◽  
...  
Keyword(s):  
Multidrug Resistant ◽  
Peptide Deformylase ◽  
Binding Modes ◽  
Proof Of Concept ◽  
Compound Structure ◽  
3 Dimensional ◽  
Design And Synthesis ◽  
Relationship Analysis

Abstract Peptide deformylase (PDF) is considered an excellent target to develop antibiotics. We have performed an extensive characterization of a new PDF from the pathogen Streptococcus agalactiae, showing properties similar to other known PDFs. S. agalactiae PDF could be used as PDF prototype as it allowed to get complete sets of 3-dimensional, biophysical and kinetic data with virtually any inhibitor compound. Structure-activity relationship analysis with this single reference system allowed us to reveal distinct binding modes for different PDF inhibitors and the key role of a hydrogen bond in potentiating the interaction between ligand and target. We propose this protein as an irreplaceable tool, allowing easy and relevant fine comparisons between series, to design, challenge and validate novel series of inhibitors. As proof-of-concept, we report here the design and synthesis of effective specific bacterial PDF inhibitors of an oxadiazole series with potent antimicrobial activity against a multidrug resistant clinical isolate.

scholarly journals Atomic-scale characterization of mature HIV-1 capsid stabilization by inositol hexakisphosphate (IP6)

2020 ◽  
Vol 6 (38) ◽  
pp. eabc6465 ◽  
Author(s):  
Alvin Yu ◽  
Elizabeth M. Y. Lee ◽  
Jaehyeok Jin ◽  
Gregory A. Voth
Keyword(s):  
Kinetic Studies ◽  
Binding Pocket ◽  
Free Energy Calculations ◽  
Atomic Scale ◽  
Binding Modes ◽  
Ligand Density ◽  
Scale Characterization ◽  
Dynamics Simulations

Inositol hexakisphosphates (IP6) are cellular cofactors that promote the assembly of mature capsids of HIV. These negatively charged molecules coordinate an electropositive ring of arginines at the center of pores distributed throughout the capsid surface. Kinetic studies indicate that the binding of IP6 increases the stable lifetimes of the capsid by several orders of magnitude from minutes to hours. Using all-atom molecular dynamics simulations, we uncover the mechanisms that underlie the unusually high stability of mature capsids in complex with IP6. We find that capsid hexamers and pentamers have differential binding modes for IP6. Ligand density calculations show three sites of interaction with IP6 including at a known capsid inhibitor binding pocket. Free energy calculations demonstrate that IP6 preferentially stabilizes pentamers over hexamers to enhance fullerene modes of assembly. These results elucidate the molecular role of IP6 in stabilizing and assembling the retroviral capsid.

Resonance Raman characterization of water-soluble Co-porphyrins and their complexes with nucleic acids

1999 ◽  
pp. 283-284 ◽  
Author(s):  
S. N. Terekhov ◽  
V. A. Galievsky ◽  
V. S. Chirvony ◽  
S. G. Kruglik ◽  
V. V. Ermolenkov ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Resonance Raman ◽  
Water Soluble ◽  
Raman Characterization

Binding modes and base specificity of tris(phenanthroline)ruthenium(II) enantiomers with nucleic acids: tuning the stereoselectivity

1986 ◽  
Vol 108 (8) ◽  
pp. 2081-2088 ◽  
Author(s):  
Jacqueline K. Barton ◽  
Jonathan M. Goldberg ◽  
Challa V. Kumar ◽  
Nicholas J. Turro
Keyword(s):  
Nucleic Acids ◽  
Binding Modes ◽  
Base Specificity

Digital x-ray mapping of nanometer-size supported bimetallic catalysts

1988 ◽  
Vol 46 ◽  
pp. 530-531
Author(s):  
L. T. Germinario
Keyword(s):  
Background Radiation ◽  
Metal Cluster ◽  
Single Element ◽  
Beam Diameter ◽  
X Rays ◽  
X Ray ◽  
Major Interest ◽  
Induced Changes

Understanding the role of metal cluster composition in determining catalytic selectivity and activity is of major interest in heterogeneous catalysis. The electron microscope is well established as a powerful tool for ultrastructural and compositional characterization of support and catalyst. Because the spatial resolution of x-ray microanalysis is defined by the smallest beam diameter into which the required number of electrons can be focused, the dedicated STEM with FEG is the instrument of choice. The main sources of errors in energy dispersive x-ray analysis (EDS) are: (1) beam-induced changes in specimen composition, (2) specimen drift, (3) instrumental factors which produce background radiation, and (4) basic statistical limitations which result in the detection of a finite number of x-ray photons. Digital beam techniques have been described for supported single-element metal clusters with spatial resolutions of about 10 nm. However, the detection of spurious characteristic x-rays away from catalyst particles produced images requiring several image processing steps.

Consideraciones en torno a la reducción solipsista

1970 ◽  
Vol 11 ◽  
Author(s):  
Natalia Carolina Petrillo

ResumenEn el presente trabajo se intentará mostrar que la fenomenología no conduce a una postura solipsista. Para ello, se caracterizará en qué consiste el solipsismo. Luego, se intentará refutar a lo que se ha de llamar “solipsismo metafísico” y “solipsismo gnoseológico”, con el objetivo principal de poner de manifiesto el fundamento de motivación para la salida de la ficción solipsista.Palabras claves:Phenomenology – solipsim – empatía - HusserlAbstractWith the aim of showing that phenomenology does not lead in solipsism, I will first attempt a characterization of it. Then, I will attempt a refutation of the so-called “metaphysical” and “epistemological” solipsisms. Finally, the nature and role of Husserl´s solipsistic fiction is examined, and the grounds that motivate the overcoming of this standpoint are disclosed.key wordsFenomenología – solipsismo - empathy – Husserl

ART STUDIOS – A WAY TO DEVELOPE CHILDREN’S IMAGINATION

2020 ◽  
Vol 11 (1) ◽  
pp. 144-148
Author(s):  
Liuba Zlatkova ◽  
Keyword(s):  
Fairy Tale ◽  
Psychological Effects ◽  
Complete Product ◽  
The Arts ◽  
The Creation ◽  
Role Of Parents

The report describes the steps for creating a musical tale by children in the art studios of „Art Workshop“, Shumen. These studios are led by students volunteers related to the arts from pedagogical department of Shumen University, and are realized in time for optional activities in the school where the child studies. The stages of creating a complete product with the help of different arts are traced – from the birth of the idea; the creation of a fairy tale plot by the children; the characterization of the fairy-tale characters; dressing them in movement, song and speech; creating sets and costumes and creating a finished product to present on stage. The role of parents as a link and a necessary helper for children and leaders is also considered, as well as the positive psychological effects that this cooperation creates.

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