Detailed analysis of single-molecule surface-enhanced resonance Raman scattering spectra of Rhodamine 6G obtained from isolated nano-aggregates of colloidal silver

2005 ◽  
Vol 36 (6-7) ◽  
pp. 593-599 ◽  
Author(s):  
Slobodan Šašić ◽  
Tamitake Itoh ◽  
Yukihiro Ozaki
Keyword(s):  
Raman Scattering ◽  
Detailed Analysis ◽  
Single Molecule ◽  
Rhodamine 6G ◽  
Colloidal Silver ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Scattering Spectra ◽  
Raman Scattering Spectra ◽  
Molecule Surface

Approach to Single Molecule Detection Using Surface-Enhanced Resonance Raman Scattering (SERRS): A Study Using Rhodamine 6G on Colloidal Silver

1995 ◽  
Vol 49 (6) ◽  
pp. 780-784 ◽  
Author(s):  
Katrin Kneipp ◽  
Yang Wang ◽  
Ramachandra R. Dasari ◽  
Michael S. Feld
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Rhodamine 6G ◽  
Detection System ◽  
Resonance Raman ◽  
Single Molecule Detection ◽  
Colloidal Silver ◽  
Fiber Optic Probe ◽  
Resonance Raman Scattering ◽  
Surface Enhanced

We have measured surface-enhanced resonance Raman scattering (SERRS) spectra of rhodamine 6G (R6G) at concentrations as low as 8 × 10−16 M in colloidal silver solution activated by NaCl ions. The spectra were measured with a fiber-optic probe using the 514.5-nm argon-ion laser line as excitation source and a charge-coupled-device (CCD) detection system. The correlation of SERRS photo counts and R6G concentration was found to be linear between 8 × 10−11 and 8 × 10−14 M concentrations within our experimental accuracy. Experiments conducted with small scattering volumes show that fewer than 100 R6G molecules are sufficient to give rise to a SERRS spectrum with reasonable signal-to-noise ratio. These results demonstrate that in certain cases SERRS can achieve detection limits comparable to those for fluorescence spectroscopy, and at the same time provides higher structural specificity than fluorescence. The possibilities of using SERRS for single molecule detection are discussed.

Comment on “Sensitive marker bands for the detection of spin states of heme in surface-enhanced resonance Raman scattering spectra of metmyoglobin” by Y. Kitahama, M. Egashira, T. Suzuki, I. Tanabe and Y. Ozaki

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6145-6146 ◽  
Author(s):  
A. Feis ◽  
G. Smulevich
Keyword(s):  
Silver Nanoparticles ◽  
Raman Scattering ◽  
Resonance Raman ◽  
Spin States ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Scattering Spectra ◽  
Resonance Raman Spectra ◽  
Raman Scattering Spectra ◽  
Sensitive Marker

We contrast recently reported surface-enhanced resonance Raman spectra (SERRS) of myoglobin on silver nanoparticles with established knowledge about this complex.

scholarly journals 2P312 Localized single molecule surface enhanced resonance raman scattering

2005 ◽  
Vol 45 (supplement) ◽  
pp. S197
Author(s):  
K. Watanabe ◽  
M. Tsunoda ◽  
T. Ueno ◽  
M. Yamagishi ◽  
T. Funatsu
Keyword(s):  
Raman Scattering ◽  
Single Molecule ◽  
Resonance Raman ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Molecule Surface

Reply to the comment on “Sensitive marker bands for the detection of spin states of heme in surface-enhanced resonance Raman scattering spectra of metmyoglobin”

The Analyst ◽  
2015 ◽  
Vol 140 (17) ◽  
pp. 6147-6148 ◽  
Author(s):  
Yasutaka Kitahama ◽  
Masatoshi Egashira ◽  
Toshiaki Suzuki ◽  
Ichiro Tanabe ◽  
Yukihiro Ozaki
Keyword(s):  
Raman Scattering ◽  
High Spin ◽  
Spin State ◽  
High Spin State ◽  
Spin States ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Scattering Spectra ◽  
Raman Scattering Spectra ◽  
Sensitive Marker

In our SERRS spectra, the peaks at 1510 cm−1, which are assigned not to the non-native 5-coordinated heme in the high spin state, but to the native 6-coordinated heme in the low spin state (6cLS), were observed.

Sensitive marker bands for the detection of spin states of heme in surface-enhanced resonance Raman scattering spectra of metmyoglobin

The Analyst ◽  
2014 ◽  
Vol 139 (24) ◽  
pp. 6421-6425 ◽  
Author(s):  
Yasutaka Kitahama ◽  
Masatoshi Egashira ◽  
Toshiaki Suzuki ◽  
Ichiro Tanabe ◽  
Yukihiro Ozaki
Keyword(s):  
Raman Scattering ◽  
Intensity Ratio ◽  
Resonance Raman ◽  
Spin States ◽  
Resonance Raman Scattering ◽  
Surface Enhanced ◽  
Scattering Spectra ◽  
Raman Scattering Spectra ◽  
Sensitive Marker

The SERRS intensity ratio of the peak at 1560 cm−1to that at 1620 cm−1was applied to detect the spin states of heme in metmyoglobin sensitively.

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