Pharmacodynamic Interaction of Recombinant Human Interleukin-10 and Prednisolone Using in vitro Whole Blood Lymphocyte Proliferation

2002 ◽  
Vol 91 (5) ◽  
pp. 1334-1342 ◽  
Author(s):  
Abhijit Chakraborty ◽  
William J. Jusko
Keyword(s):  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Interleukin 10 ◽  
Pharmacodynamic Interaction

scholarly journals Pharmacokinetic/pharmacodynamic model for prednisolone inhibition of whole blood lymphocyte proliferation

2002 ◽  
Vol 53 (5) ◽  
pp. 474-484 ◽  
Author(s):  
Mindy He Magee ◽  
Robert A. Blum ◽  
Christian D. Lates ◽  
William J. Jusko
Keyword(s):  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Pharmacodynamic Model

scholarly journals Whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune responses in bovine brucellosis

1978 ◽  
Vol 7 (6) ◽  
pp. 550-557
Author(s):  
J M Kaneene ◽  
R K Anderson ◽  
D W Johnson ◽  
C C Muscoplat ◽  
P Nicoletti ◽  
...  
Keyword(s):  
Immune Response ◽  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Stimulation ◽  
Soluble Antigen ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Cell Mediated Immune Response ◽  
Stimulation Tests

A study was conducted to develop an in vitro whole-blood lymphocyte stimulation assay for measurement of cell-mediated immune response in bovine brucellosis. A soluble antigen (BASA) prepared from killed cells of Brucella abortus 1119-3 was used. Cattle infected with B. abortus field strains, B. abortus 19 calfhood- and adult-vaccinated cattle, and nonexposed cattle were tested. Blood was diluted 10-fold in RPMI-1640 medium (without added serum) and cultured with BASA (at a concentration of 2.2 microgram per culture) at varying times of incubation. Results were assayed for [3H]thymidine incorporation into deoxyribonucleic acid. A 6-day period was found to be optimal for incubating blood cultures to achieve maximum specific lymphocyte stimulation. Serological tests and bacteriological isolation attempts were conducted simultaneously with lymphocyte stimulation tests, and there was a significant correlation between cell-mediated immune response and bacteriological findings. There was a significant correlation between cell-mediated immune response and the level of serum antibodies on a group basis, but there was little correlation between the two systems on individual infected animals. Among vaccinated animals there was little or no correlation between cell-mediated immune and humoral responses. The whole-blood assay was found to be simple, fast, sensitive, and reproducible.

scholarly journals Comparison of sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle

1978 ◽  
Vol 8 (4) ◽  
pp. 396-401
Author(s):  
J M Kaneene ◽  
D W Johnson ◽  
R K Anderson ◽  
C C Muscoplat
Keyword(s):  
Sensitivity And Specificity ◽  
Brucella Abortus ◽  
Whole Blood ◽  
Blood Lymphocyte ◽  
Thymidine Incorporation ◽  
Lymphocyte Stimulation ◽  
Soluble Antigen ◽  
Serum Supplement ◽  
Brucella Infection

A study was conducted to compare the sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. Cattle used were infected with B. abortus field strains or strain 19. Peripheral blood was collected, and lymphocytes for the technique. The blood for the whole-blood lymphocyte stimulation assay was diluted 10-fold with RPMI 1640 medium (without additional serum supplement) and cultured. The two tests were run simultaneously, and B. abortus soluble antigen or concanavalin A was added to the cultures. The cultures were incubated for 6 days and assayed for [3H] thymidine incorporation into their DNA. Generally, cultures of the purified lymphocyte stimulation assay had higher counts per minute than those of the whole-blood lymphocyte stimulation assay, but the stimulation ratios for the two tests were comparable. The two assays were comparable in terms of their sensitivity and specificity as applied to detection of brucella infection in cattle.

scholarly journals Interactions of Everolimus and Sorafenib in Whole Blood Lymphocyte Proliferation

2012 ◽  
Vol 30 (3) ◽  
pp. 707-713 ◽  
Author(s):  
Dipti K. Pawaskar ◽  
Robert M. Straubinger ◽  
Gerald J. Fetterly ◽  
Wen W. Ma ◽  
William J. Jusko
Keyword(s):  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Proliferation

Optimizing whole blood lymphocyte proliferation in the rat

1995 ◽  
Vol 184 (2) ◽  
pp. 163-167 ◽  
Author(s):  
Adedigbo A. Fasanmade ◽  
William J. Jusko
Keyword(s):  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Proliferation

scholarly journals Endometrial mesenchymal stem/stromal cell modulation of T cell proliferation

Reproduction ◽  
2018 ◽  
Author(s):  
Xiaoqing Yang ◽  
Meivita Devianti ◽  
Yuan H Yang ◽  
Yih Rue Ong ◽  
Ker Sin Tan ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Regulatory T Cells ◽  
Immune Responses ◽  
Stromal Cells ◽  
Lymphocyte Proliferation ◽  
Interleukin 10 ◽  
Immunomodulatory Properties ◽  
Tgf Β1

Perivascular mesenchymal stem/stromal cells can be isolated from the human endometrium using the surface marker SUSD2, and are being investigated for use in tissue repair. Mesenchymal stem/stromal cells from other tissues modulate T cell responses via mechanisms including interleukin-10, prostaglandin E2, TGF-β1 and regulatory T cells. Animal studies demonstrate that endometrial mesenchymal stem/stromal cells can also modify immune responses to implanted mesh, but the mechanism/s they employ have not been explored. We examined the immunomodulatory properties of human endometrial mesenchymal stem/stromal cells on lymphocyte proliferation using mouse splenocyte cultures. Endometrial mesenchymal stem/stromal cells inhibited mitogen-induced lymphocyte proliferation in vitro in a dose-dependent manner. Inhibition of lymphocyte proliferation was not affected by blocking the mouse interleukin-10 receptor or inhibiting prostaglandin production. Endometrial mesenchymal stem/stromal cells continued to restrain lymphocyte proliferation in the presence of an inhibitor of TGF-β receptors, despite a reduction in regulatory T cells. Thus the in vitro inhibition of mitogen-induced lymphocyte proliferation by endometrial mesenchymal stem/stromal cells occurs by a mechanism distinct from the interleukin-10, prostaglandin E2, TGF-β1, and regulatory T cell-mediated mechanisms employed by MSC from other tissues. eMSC were shown to produce interleukin-17A and Dickkopf-1 which may contribute to their immunomodulatory properties. In contrast to MSC from other sources, systemic administration of endometrial mesenchymal stem/stromal cells did not inhibit swelling in a T cell-mediated model of skin inflammation. We conclude that, while endometrial mesenchymal stem/stromal cells can modify immune responses, their immunomodulatory repertoire may not be sufficient to restrain some T cell-mediated inflammatory events.

More on genetic predisposition

1995 ◽  
Vol 14 (12) ◽  
pp. 992-993
Author(s):  
Richard C Strange
Keyword(s):  
Sister Chromatid ◽  
Whole Blood ◽  
Blood Lymphocyte ◽  
Human Lymphocytes ◽  
Bimodal Distribution ◽  
Sister Chromatid Exchanges ◽  
Gstt1 Null Genotype ◽  
Lymphocyte Cultures ◽  
Chromatid Exchanges

The individual genotoxic response of cultured human lymphocytes to diepoxybutane (DEB), an epoxide metabolite of 1,3-butadiene, shows a bimodal distribution. Blood donors can be classi fied as either DEB-sensitive or DEB-resistant on the basis of the frequency of sister chromatid exchanges (SCEs) induced by DEB in whole-blood lymphocyte cultures. The genetic basis of this phenomenon has thus far been unknown. To investigate if differences in the ability of individuals to detoxify DEB could explain the bimodal response, sister chromatid exchanges (SCEs) induced by a 48-h treatment with DEB (2 and 5 μM were analysed in whole-blood lymphocyte cultures of 20 human donors with known genotypes of two polymorphic glutathione S-transferases (GSTs). GSTT1 and GSTM1. Both polymorphisms include a homozygous null geno type lacking the respective GST gene and isozyme. The mean frequency of SCEs/cell was 1.6 times higher among GSTT1 null donors (n = 8) than GSTT1 positive donors (n = 12) at both 2 μM DEB (mean 67.3 versus 40.9) and 5 μM DEB (mean 123.2 versus 77.5), with no overlapping in DEB-induced individual SCE frequencies between the two geno types. Thus, all DEB-sensitive individuals were of the GSTT1 null genotype, while all DEB-resistant persons had a detectable GSTT1 gene. A significant (P < 0.05) negative correlation (r = -0.65 at 5 μM, r = 0.56 at 2 μM) was obtained in the GSTT1 positive donors between DEB-induced individual SCE fre quency and RBC GSTT1 activity, measured by formaldehyde formation from dichloromethane; the GSTT1 null individuals showed no GSTT1 activity. At 5 μM DEB, the lymphocyte cultures of the GSTT1 null donors also had a significantly decreased repli cation index, indicating an impact of GSTT1 geno type on the cytotoxicity of DEB. No influence on DEB-induced SCEs or cytotoxic effects was observed for GSTM1 genotype. It is concluded that sensitivity to in vitro SCE induction by DEB is explained by the lack of GSTT1.

Effect of Anesthesia on a Whole Blood Lymphocyte Proliferation Assay in the Rat

1999 ◽  
Vol 21 (2) ◽  
pp. 267-276 ◽  
Author(s):  
Jan F. Gummed ◽  
Glen Otto ◽  
Markus J. Barten ◽  
Randall E. Morris
Keyword(s):  
Whole Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Proliferation ◽  
Lymphocyte Proliferation Assay ◽  
Proliferation Assay
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