General Treatment of Competitive Binding as Applied to the Potentiometric Ion Probe Technique: Application to the Interaction of Nonsteroidal Anti- Inflammatory Drugs with Bovine Serum Albumin

1994 ◽  
Vol 83 (8) ◽  
pp. 1150-1154 ◽  
Author(s):  
Alexandra Th. Angelakou ◽  
Evangelos E. Sideris ◽  
Georgia N. Valsami ◽  
Michael A. Koupparis ◽  
Panos E. Macheras
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Competitive Binding ◽  
General Treatment ◽  
Probe Technique ◽  
Ion Probe ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

scholarly journals Displacement of sulfonamides from bovine serum albumin by nonsteroidal anti-inflammatory drugs.

1979 ◽  
Vol 27 (6) ◽  
pp. 1335-1342 ◽  
Author(s):  
YOSHIHARU KANEO ◽  
ATSUKO NISHIKAWA ◽  
YURIKO KATO ◽  
SETSUO KIRYU
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Mechanistic and conformational studies on the interaction of anti-inflammatory drugs, isoxicam and tenoxicam with bovine serum albumin

2010 ◽  
Vol 130 (11) ◽  
pp. 2052-2058 ◽  
Author(s):  
Reeta Punith ◽  
Umesha Katrahalli ◽  
Shankara S. Kalanur ◽  
Seetharamappa Jaldappagari
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Conformational Studies ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

scholarly journals Pharmacological and ADMET-based pharmacokinetic properties of Syzygium samarangense var. parviflorum leaf extract in in vitro, in vivo and in silico models

2020 ◽  
Vol 48 (3) ◽  
pp. 1155-1175
Author(s):  
Rahni HOSSAIN ◽  
Md A. RAHMAN ◽  
Md. K. J. RAFI ◽  
Tanvir A. SIDDIQUE ◽  
Abdullah A. NOMAN ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Computational Models ◽  
Clot Lysis ◽  
Pharmacological Properties ◽  
Reference Drug ◽  
Egg Albumin ◽  
Anti Inflammatory

This research investigated pharmacological properties mainly the anti-inflammatory, anthelmintic, thrombolytic and anxiolytic potential of methanol extract of Syzygium samarangense (MESS) var. parviflorum. Anti-inflammatory action by bovine serum albumin, egg albumin denaturation and membrane stabilization, anthelmintic by live parasites, thrombolytic by clot lysis and anxiolytic by elevated plus maze (EPM) and light and dark box (LDB) tests were measured. The four targeted pharmacological properties were further justified using the most prevalent compounds, isolated from this plant, to be undergone for their pharmacokinetic property’s analyses, sitemap analyses and lignad-receptor interactions by computational models through SwissADME and Schrödinger, 2018 softwares against PDB 6COX, 6D6T, 1JFF receptors. MESS was found to display statistically significant (P < 0.05) inhibition of Bovine Serum albumin and Egg albumin denaturation compared to reference drug diclofenac sodium. Remarkable vermicidal effect on the paralysis and death of anthelmintic parasites was observed at MESS concentration 200 mg/dL. A nondescript clot lysis of MESS compared to streptokinase was evident in in vitro thrombolytic assay. MESS increased the number of times the animal crossed from one compartment to the other and the time spent in the brightly-lit chamber of the LDB. Three-methylchalcone derivatives out of seven MESS compounds were undertaken, based on cut off value and sitemap prediction score, for further ligand-receptor binding efficiency. All these three compounds showed promising docking score, glide emodel and glide energy against PDB 6COX, 6D6T and 1DDJ, plasmin proteins demonstrating the prospects of MESS to be materialized for anti-inflammatory, anthelmintic, and thrombolytic therapeutics with further clarification.

Antibodies to Testosterone-3-Bovine Serum Albumin, Applied to Assay of Serum 17β-ol Androgens

1971 ◽  
Vol 17 (7) ◽  
pp. 581-584 ◽  
Author(s):  
Jeffrey C Chen ◽  
Elinor M Zorn ◽  
Marvin C Hallberg ◽  
Ralph G Wieland
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Binding Assay ◽  
Virgin Female ◽  
Competitive Binding ◽  
Competitive Binding Assay ◽  
Menstruating Women ◽  
Normal Men ◽  
Mean Concentration

Abstract Testosterone was conjugated to bovine serum albumin at carbon 3, and antibodies to this conjugate were developed in virgin female rabbits. This antiserum, diluted 1:3200, was used to measure testosterone. With tritiated testosterone and anti-rabbit γ-globulin as the second antibody, a doseresponse curve was obtained for doses in the range 0.1-10 ng. The antibody cross-reacts extensively with dihydrotestosterone, so that dihydrotestosterone is measured with testosterone in extracted but unchromatographed serum. Results for pooled serum from men, as measured repeatedly by radioimmunoassay and competitive binding assay for testosterone, agreed well. Seventeen normal men had serum 17β-ol androgen concentrations of 667 ng/100 ml (SD, ±248); seventeen normal, menstruating women had a mean concentration of 69 ng/100 ml (SD, ±18). Values for 17β-ol androgens in adolescent males agreed reasonably with testosterone values reported by other investigators. Values are presented for patients with various androgenic disorders. This procedure is easy; many samples can be processed at one time.

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