Mechanism of Action of Granaticin: Inhibition of Ribosomal RNA Maturation and Cell Cycle Specificity

1982 ◽  
Vol 71 (2) ◽  
pp. 197-200 ◽  
Author(s):  
Peter Heinstein
Keyword(s):  
Cell Cycle ◽  
Ribosomal Rna ◽  
Mechanism Of Action ◽  
Rna Maturation

mTOR function in skeletal muscle hypertrophy: increased ribosomal RNA via cell cycle regulators

2005 ◽  
Vol 289 (6) ◽  
pp. C1457-C1465 ◽  
Author(s):  
Gustavo A. Nader ◽  
Thomas J. McLoughlin ◽  
Karyn A. Esser
Keyword(s):  
Cell Cycle ◽  
Protein Expression ◽  
Cyclin D1 ◽  
Ribosomal Rna ◽  
Molecular Mechanisms ◽  
D1 Protein ◽  
Rna Content ◽  
Cyclin D1 Protein ◽  
Rb Phosphorylation ◽  
Myotube Hypertrophy

The purpose of this study was to identify the potential downstream functions associated with mammalian target of rapamycin (mTOR) signaling during myotube hypertrophy. Terminally differentiated myotubes were serum stimulated for 3, 6, 12, 24, and 48 h. This treatment resulted in significant myotube hypertrophy (protein/DNA) and increased RNA content (RNA/DNA) with no changes in DNA content or indices of cell proliferation. During myotube hypertrophy, the increase in RNA content was accompanied by an increase in tumor suppressor protein retinoblastoma (Rb) phosphorylation and a corresponding increase in the availability of the ribosomal DNA transcription factor upstream binding factor (UBF). Serum stimulation also induced an increase in cyclin D1 protein expression in the differentiated myotubes with a concomitant increase in cyclin D1-dependent cyclin-dependent kinase (CDK)-4 activity toward Rb. The increases in myotube hypertrophy and RNA content were blocked by rapamycin treatment, which also prevented the increase in cyclin D1 protein expression, CDK-4 activity, Rb phosphorylation, and the increase in UBF availability. Our findings demonstrate that activation of mTOR is necessary for myotube hypertrophy and suggest that the role of mTOR is in part to modulate cyclin D1-dependent CDK-4 activity in the regulation of Rb and ribosomal RNA synthesis. On the basis of these results, we propose that common molecular mechanisms contribute to the regulation of myotube hypertrophy and growth during the G1 phase of the cell cycle.

scholarly journals Ribosomal RNA: Maturation in a Missing Link

Nature ◽  
1970 ◽  
Vol 227 (5257) ◽  
pp. 441-441
Author(s):  
Keyword(s):  
Ribosomal Rna ◽  
Missing Link ◽  
Rna Maturation

Control of ribosomal RNA maturation in differentiating yolk sac erythroid cells

1972 ◽  
Vol 1 (4) ◽  
pp. 219-228 ◽  
Author(s):  
Antonio Fantoni ◽  
Sandra Bordin ◽  
Mario Lunadei
Keyword(s):  
Ribosomal Rna ◽  
Yolk Sac ◽  
Erythroid Cells ◽  
Rna Maturation

scholarly journals A Robust High-Content Imaging Approach for Probing the Mechanism of Action and Phenotypic Outcomes of Cell-Cycle Modulators

2011 ◽  
Vol 10 (2) ◽  
pp. 242-254 ◽  
Author(s):  
Jeffrey J. Sutherland ◽  
Jonathan Low ◽  
Wayne Blosser ◽  
Michele Dowless ◽  
Thomas A. Engler ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mechanism Of Action ◽  
High Content Imaging ◽  
Imaging Approach

Abstract 3411: Mathematical model of the cell cycle to determine mechanism of action and optimize dosing schedule.

2013 ◽  
Author(s):  
Jerome Mettetal ◽  
Derek Blair ◽  
Esha Gangoli ◽  
Patrick Vincent ◽  
Jeff Ecsedy ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Cell Cycle ◽  
Mechanism Of Action ◽  
Dosing Schedule ◽  
Determine Mechanism

scholarly journals Deciphering the mechanism of action of 089, a compound impairing the fungal cell cycle

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Irene Stefanini ◽  
Lisa Rizzetto ◽  
Damariz Rivero ◽  
Silvia Carbonell ◽  
Marta Gut ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mechanism Of Action ◽  
Fungal Cell
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