Colorimetric Determination of Penicillins and Related Compounds in Intravenous Solutions by Nickel(II)-Catalyzed Hydroxamic Acid Formation

1979 ◽  
Vol 68 (10) ◽  
pp. 1333-1335 ◽  
Author(s):  
J.W. Munson ◽  
D. Papadimitriou ◽  
P.P. DeLuca
Keyword(s):  
Hydroxamic Acid ◽  
Acid Formation ◽  
Colorimetric Determination ◽  
Related Compounds

DETERMINATION OF GLYCEROL AND RELATED COMPOUNDS IN FERMENTATION SOLUTIONS BY PARTITION CHROMATOGRAPHY

1950 ◽  
Vol 28b (9) ◽  
pp. 535-540 ◽  
Author(s):  
A. C. Neish
Keyword(s):  
Aqueous Solution ◽  
Periodate Oxidation ◽  
Colorimetric Determination ◽  
Partition Chromatography ◽  
Sugar Alcohols ◽  
Modified Technique ◽  
Silica Column ◽  
To Receive ◽  
Related Compounds

A modified technique is described for applying partition chromatography, using a silica column, to the separation of simple organic compounds which are difficult to extract from water. An aqueous solution is pipetted directly on a Celite–water column the upper 15% of which has been left dry to receive it. Acetoin, 2, 3-butanediol, 1, 2-propanediol, ethanediol, glycerol, and erythritol can be separated from each other and from sugars and sugar alcohols containing five or more carbon atoms if the column is properly developed by ethyl acetate and benzene–butanol mixtures. After separation the compounds can be estimated by a rapid colorimetric determination of the acetaldehyde or formaldehyde formed by periodate oxidation. The recoveries are quantitative, thus making it possible to determine 1 to 2 mgm. of glycerol or 2, 3-butanediol within an error of ± 3% even when they are mixed with larger amounts of closely related compounds which cause interference in the usual methods.

scholarly journals Rat Serum Carboxylesterase Partly Hydrolyses Gamma-Butyrobetaine Esters

2009 ◽  
Vol 60 (2) ◽  
pp. 147-156 ◽  
Author(s):  
Lida Bagdonienė ◽  
Danutė Labeikytė ◽  
Ivars Kalviņš ◽  
Veronika Borutinskaitė ◽  
Aleksandrs Prokofjevs ◽  
...  
Keyword(s):  
Blood Serum ◽  
Esterase Activity ◽  
Colorimetric Determination ◽  
Reaction Products ◽  
Rat Serum ◽  
Rat Blood ◽  
Optical Absorbance ◽  
Hydrolysis Of ◽  
Related Compounds

Rat Serum Carboxylesterase Partly Hydrolyses Gamma-Butyrobetaine EstersAlthough described some time ago, gamma-butyrobetaine esters and related compounds have not gained much attention from researchers, and their physiological function remains obscure. Formerly we detected GBB-esterase enzymatic activity in rat blood serum using phenylated gamma-butyrobetaine as an artificial substrate of the enzyme and HPLC. The aim of the present work was to develop an assay that would enable spectrophotometric or colorimetric determination of the reaction products of GBB-esterase activity and to reveal individual proteins performing GBB-esterase activity in rat blood serum. For this purpose gamma-butyrobetaine 1-naphthyl ester was synthesised. Hydrolysis of this ester releases 1-naphthol, which increases the optical absorbance at 322 nm. We have shown that the enzymatic hydrolysis of GBB 1-naphthyl ester to 1-naphthol in rat blood serum is due to GBB-esterase activity. An attempt was done to purify the enzyme from rat blood serum. By combining DEAE Sepharose at pH 4.2 and affinity chromatography with procainamide we achieved a 68-fold enrichment of GBB-esterase activity in our preparations. Separation of fraction proteins in 2D protein electrophoresis with following mass-spectrometry indicated that GBB esterase activity in rat blood serum is performed in part by carboxylesterase.

scholarly journals Quantitative colorimetric determination of urinary p-aminophenol with an automated analyzer

1997 ◽  
Vol 43 (4) ◽  
pp. 627-634 ◽  
Author(s):  
Jan F Van Bocxlaer ◽  
Karine M Clauwaert ◽  
Willy E Lambert ◽  
André P De Leenheer
Keyword(s):  
Colorimetric Method ◽  
Stability Study ◽  
Colorimetric Determination ◽  
Manganese Ions ◽  
Significant Interference ◽  
Related Compounds ◽  
Health Screen ◽  
Linear Concentration Range ◽  
Linear Concentration

Abstract We developed an automated colorimetric method for the quantitative determination of p-aminophenol with a Cobas Mira analyzer. The procedure can be used for the biological monitoring of human exposure to aniline. An absorbed aniline dose is extensively oxidized to p-aminophenol, which is excreted in urine mainly as glucurono- and sulfo- conjugates. After enzymatic hydrolysis, we reacted the free compound with resorcinol in the presence of manganese ions to form an indophenol dye, which is measured at 550 nm. Excellent accuracy (102.8%, 103.9%, and 96.8% at 2.5, 50, and 90 mg/L, respectively) and precision (7.7%, 2.1%, and 0.8% CV for within-run and 11.1%, 4.7%, and 4.6% for total reproducibility at 2.5, 50, and 90 mg/L, respectively) were achieved over a linear concentration range of 2.0 to 100 mg/L. The detection limit was 0.9 mg/L and no significant interference (except for o-aminophenol) was found for several investigated drugs and related compounds. The proposed method was used for a stability study and to analyze several samples from an occupational health screen.

Colorimetric Determination of 1,2-Propanediol and Related Compounds

1957 ◽  
Vol 29 (8) ◽  
pp. 1214-1216 ◽  
Author(s):  
L. R. Jones ◽  
J. A. Riddick
Keyword(s):  
Colorimetric Determination ◽  
Related Compounds
Sign in / Sign up

Export Citation Format

Share Document