Determination of Polythiazide in Pharmaceutical Dosage Forms by High-Pressure Liquid Chromatography

1975 ◽  
Vol 64 (8) ◽  
pp. 1406-1408 ◽  
Author(s):  
R.E. Moskalyk ◽  
R.A. Locock ◽  
L.G. Chatten ◽  
Monique F. Bielech ◽  
A.M. Veltman
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
High Pressure Liquid Chromatography ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Forms

Simultaneous Determination of Vitamins A and D in Dosage Forms by High Pressure Liquid Chromatography

1982 ◽  
Vol 65 (3) ◽  
pp. 619-623
Author(s):  
Maria Ines ◽  
R M Santoro ◽  
João F Magalhães ◽  
Erika R M Hackmann
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Vitamin A ◽  
Petroleum Ether ◽  
High Pressure Liquid Chromatography ◽  
Mobile Phase ◽  
Dosage Forms ◽  
Reverse Phase ◽  
Solvent Residues

Abstract Vitamins A and D were determined simultaneously in oily solutions, ointments, and elixirs, but only vitamin A could be determined in capsules. Samples were saponified with KOH in isopropanol-water, using hydroquinone as antioxidant, and extracted with ether-petroleum ether (1 + 1). After evaporation of solvent, residues were dissolved in isopropanol. Vitamins in these solutions were determined by reverse phase high pressure liquid chromatography, using methanol-water as mobile phase and detection at 254 nm. The reproducibility, using external standards, was 1.6-2.5% and 1.2-3.8% for vitamins A and D, respectively.

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