scholarly journals Biophysical Characterization of the Type III Secretion Tip Proteins and the Tip Proteins Attached to Bacterium-Like Particles

2015 ◽  
Vol 104 (2) ◽  
pp. 424-432 ◽  
Author(s):  
Shyamal P. Choudhari ◽  
Xiaotong Chen ◽  
Jae Hyun Kim ◽  
Maarten L. Van Roosmalen ◽  
Jamie C. Greenwood ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Biophysical Characterization ◽  
Type Iii

Biophysical Characterization of the Type III Secretion System Translocator Proteins and the Translocator Proteins Attached to Bacterium-Like Particles

2015 ◽  
Vol 104 (12) ◽  
pp. 4065-4073 ◽  
Author(s):  
Xiaotong Chen ◽  
Shyamal P. Choudhari ◽  
Prashant Kumar ◽  
Ronald T. Toth ◽  
Jae Hyun Kim ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Biophysical Characterization ◽  
Type Iii

scholarly journals Characterization of the Yersinia enterocolitica Type III Secretion ATPase YscN and Its Regulator, YscL

2006 ◽  
Vol 188 (10) ◽  
pp. 3525-3534 ◽  
Author(s):  
Bill Blaylock ◽  
Kelly E. Riordan ◽  
Dominique M. Missiakas ◽  
Olaf Schneewind
Keyword(s):  
Yersinia Enterocolitica ◽  
Type Iii Secretion ◽  
Atp Hydrolysis ◽  
Biochemical Properties ◽  
Host Cells ◽  
Gram Negative Bacteria ◽  
Type Iii ◽  
Bacterial Proteins

ABSTRACT Type III secretion is a mechanism used by a broad range of gram-negative bacteria to neutralize eukaryotic defenses by enabling translocation of bacterial proteins directly into the cytoplasm of host cells. The bacterial energy source for secretion is ATP, which is consumed by an ATPase that couples ATP hydrolysis to the unfolding of secreted proteins and the dissociation of their chaperones just prior to secretion. By studying the biochemical properties of YscN and YscL of Yersinia enterocolitica, we have characterized them as the ATPase and ATPase regulator, respectively, of the type III secretion system of this organism. In vivo, YscL and YscN interact with each other, and the overexpression of glutathione S-transferase-YscL abolishes secretion and down-regulates the expression of secretion apparatus components.

Evaluation of biofilm production and characterization of genes encoding type III secretion system among Pseudomonas aeruginosa isolated from burn patients

Burns ◽  
2012 ◽  
Vol 38 (8) ◽  
pp. 1192-1197 ◽  
Author(s):  
Fereshteh Jabalameli ◽  
Akbar Mirsalehian ◽  
Babak Khoramian ◽  
Marzieh Aligholi ◽  
Seyed Sajjad Khoramrooz ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Burn Patients ◽  
Type Iii ◽  
Biofilm Production ◽  
Genes Encoding

scholarly journals Structural and Biochemical Characterization of SrcA, a Multi-Cargo Type III Secretion Chaperone in Salmonella Required for Pathogenic Association with a Host

2010 ◽  
Vol 6 (2) ◽  
pp. e1000751 ◽  
Author(s):  
Colin A. Cooper ◽  
Kun Zhang ◽  
Sara N. Andres ◽  
Yuan Fang ◽  
Natalia A. Kaniuk ◽  
...  
Keyword(s):  
Type Iii Secretion ◽  
Biochemical Characterization ◽  
Type Iii ◽  
Secretion Chaperone
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