Propagation of Uncertainty in Nasal Spray In Vitro Performance Models Using Monte Carlo Simulation: Part II. Error Propagation During Product Performance Modeling

2010 ◽  
Vol 99 (8) ◽  
pp. 3572-3578 ◽  
Author(s):  
Changning Guo ◽  
William H. Doub ◽  
John F. Kauffman
Keyword(s):  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Nasal Spray ◽  
Error Propagation ◽  
Performance Modeling ◽  
Product Performance ◽  
Performance Models ◽  
Propagation Of Uncertainty ◽  
In Vitro Performance

BeO-OSL detectors for dose measurements in cell cultures

2009 ◽  
Vol 48 (06) ◽  
pp. 227-232
Author(s):  
D. Sommer ◽  
R. Freudenberg ◽  
U. Reichelt ◽  
J. Henniger ◽  
J. Kotzerke ◽  
...  
Keyword(s):  
Cell Culture ◽  
Monte Carlo Simulation ◽  
Monte Carlo ◽  
Cell Cultures ◽  
Rate Sensitivity ◽  
Culture Vessel ◽  
Qualitative Properties ◽  
Dose Profile ◽  
Ambient Lighting

Summary Aim: The absorbed dose is an important parameter in experiments involving irradiation of cells in vitro with unsealed radionuclides. Typically, this is estimated with a model calculation, although the results thus obtained cannot be verified. Generally used real-time measurement methods are not applicable in this setting. A new detector material with in vitro suitability is the subject of this work. Methods: Optically-stimulated luminescence (OSL) dosimeters based on beryllium oxide (BeO) were used for dose measurement in cell cultures exposed to unsealed radionuclides. Their qualitative properties (e. g. energy-dependent count rate sensitivity, fading, contamination by radioactive liquids) were determined and compared to the results of a Monte Carlo simulation (using AMOS software). OSL dosimeters were tested in common cell culture setups with a known geometry. Results: Dose reproducibility of the OSL dosimeters was ± 1.5%. Fading at room temperature was 0.07% per day. Dose loss (optically-stimulated deletion) under ambient lighting conditions was 0.5% per minute. The Monte Carlo simulation for the relative sensitivity at different beta energies provided corresponding results to those obtained with the OSL dosimeters. Dose profile measurements using a 6 well plate and 14 ml PP tube showed that the geometry of the cell culture vessel has a marked influence on dose distribution with 188Re. Conclusion: A new dosimeter system was calibrated with β-emitters of different energy. It turned out as suitable for measuring dose in liquids. The dose profile measurements obtained are suitably precise to be used as a check against theoretical dose calculations.

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