scholarly journals Fabrication, characterization and in vitro evaluation of poly(D,L-lactide-co-glycolide) microparticles loaded with polyamidoamine–plasmid DNA dendriplexes for applications in nonviral gene delivery

2010 ◽  
Vol 99 (1) ◽  
pp. 368-384 ◽  
Author(s):  
Janjira Intra ◽  
Aliasger K. Salem
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Nonviral Gene Delivery ◽  
In Vitro Evaluation

Physicochemical and in vitro evaluation of cationic liposome, hyaluronic acid and plasmid DNA as pseudo-ternary complexes for gene delivery

2015 ◽  
Vol 484 ◽  
pp. 262-270 ◽  
Author(s):  
Tiago A. Balbino ◽  
Gabriela S.C. Correa ◽  
Marianna T.P. Favaro ◽  
Marcelo A.S. Toledo ◽  
Adriano R. Azzoni ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Cationic Liposome ◽  
Ternary Complexes ◽  
In Vitro Evaluation

Synthesis and in Vitro Evaluation of Glutamide-Containing Cationic Lipids for Gene Delivery

2006 ◽  
Vol 17 (6) ◽  
pp. 1530-1536 ◽  
Author(s):  
Vijaya Gopal ◽  
Tekkatte K. Prasad ◽  
Nalam M. Rao ◽  
Makoto Takafuji ◽  
Mohammed M. Rahman ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Cationic Lipids ◽  
In Vitro Evaluation

Plasmid DNA Size Does Affect Nonviral Gene Delivery Efficiency in Stem Cells

2012 ◽  
Vol 14 (2) ◽  
pp. 130-137 ◽  
Author(s):  
Sofia Ribeiro ◽  
Juergen Mairhofer ◽  
Catarina Madeira ◽  
Maria Margarida Diogo ◽  
Cláudia Lobato da Silva ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Nonviral Gene Delivery ◽  
Dna Size ◽  
Delivery Efficiency

INTRAMYOCARDIAL NONVIRAL GENE DELIVERY: THE IN VIVO EVALUATION OF TAT BASED PEPTIDES AS VECTORS FOR PLASMID DNA.

2004 ◽  
Vol 13 (3) ◽  
pp. 180
Author(s):  
Irina Hellgren ◽  
Anwar J Siddiqui ◽  
Beston Nore ◽  
Karl Henrik Grinnemo ◽  
Edvard Smith ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna ◽  
Nonviral Gene Delivery ◽  
In Vivo Evaluation

scholarly journals Gene delivery in vitro and in vivo from bioreducible multilayered polyelectrolyte films of plasmid DNA

Biomaterials ◽  
2009 ◽  
Vol 30 (5) ◽  
pp. 939-950 ◽  
Author(s):  
Jenifer Blacklock ◽  
Ye-Zi You ◽  
Qing-Hui Zhou ◽  
Guangzhao Mao ◽  
David Oupický
Keyword(s):  
Gene Delivery ◽  
Plasmid Dna

scholarly journals In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Keiichi Motoyama ◽  
Yoshihiro Nakashima ◽  
Yukihiko Aramaki ◽  
Fumitoshi Hirayama ◽  
Kaneto Uekama ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Gene Delivery ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Cationic Liposomes ◽  
Nonviral Vector ◽  
Liposomal Membrane ◽  
Transfer Activity ◽  
Potential Use

The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed the highest gene transfer activity in HepG2 cells without any significant cytotoxicity. In addition, γ-CyD enhanced the encapsulation ratio of pDNA with AF-liposomes, and also increased gene transfer activity as the entrapment ratio of pDNA into AF-liposomes was increased. γ-CyD stabilized the liposomal membrane of AF-liposomes and inhibited the release of calcein from AF-liposomes. The stabilizing effect of γ-CyD may be, at least in part, involved in the enhancing gene transfer activity of pDNA/γ-CyD/AF-liposomes. Therefore, these results suggest the potential use of γ-CyD for an enhancer of transfection efficiency of AF-liposomes.

In Vitro Design and Characterization of the Nonviral Gene Delivery Vector Iopamidol, Protamine, Ethiodized Oil Reagent

2011 ◽  
Vol 22 (10) ◽  
pp. 1457-1463.e2 ◽  
Author(s):  
Luke J. Higgins ◽  
Gloria L. Hwang ◽  
Jarrett Rosenberg ◽  
Regina H. Katzenberg ◽  
Nishita Kothary ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Nonviral Gene Delivery ◽  
Gene Delivery Vector ◽  
Delivery Vector ◽  
Ethiodized Oil

scholarly journals Bioreducible PEI-siRNA Nanocomplex for Liver Cancer Therapy: Transfection, Biodistribution, and Tumor Growth InhibitionIn Vivo

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Wei Xia ◽  
Yan Li ◽  
Bo Lou ◽  
Peijun Wang ◽  
Xiaolong Gao ◽  
...  
Keyword(s):  
Liver Cancer ◽  
Tumor Growth ◽  
Plasmid Dna ◽  
Gold Standard ◽  
Hepg2 Cells ◽  
Cancer Gene Therapy ◽  
Nonviral Gene Delivery ◽  
Surface Charges

A bioreducible polyethylenimine (SS-PEI) was successfully applied as a nonviral carrier for the delivery of plasmid DNA and VEGF-siRNAin vitroandin vivo. The SS-PEI could strongly condense DNA or siRNA into nanosized complexes (below 200 nm) with positive surface charges.In vitrotransfection experiments using GFP plasmid as gene reporter showed that the complexes of SS-PEI/DNA were able to efficiently transfect HepG2 cells, with efficiency comparable to that of polyethylenimine, a gold standard for nonviral gene delivery. Moreover, the complexes of SS-PEI/VEGF-siRNA could lead to reduced levels of VEGF protein in HepG2 cellsin vitro. Treatment with the complexes of SS-PEI/VEGF-siRNA efficiently inhibited HepG2 tumor growth in an xenograft mouse model. The data of this study imply that the SS-PEI is a potent nucleic acid carrier applicable for liver cancer gene therapy.

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