Quantitation of protein particles in parenteral solutions using micro-flow imaging

2009 ◽  
Vol 98 (9) ◽  
pp. 3058-3071 ◽  
Author(s):  
Chi-Ting Huang ◽  
Deepak Sharma ◽  
Peter Oma ◽  
Rajesh Krishnamurthy
Keyword(s):  
Flow Imaging ◽  
Micro Flow ◽  
Protein Particles

Micro–Flow Imaging and Resonant Mass Measurement (Archimedes) – Complementary Methods to Quantitatively Differentiate Protein Particles and Silicone Oil Droplets

2013 ◽  
Vol 102 (7) ◽  
pp. 2152-2165 ◽  
Author(s):  
Daniel Weinbuch ◽  
Sarah Zölls ◽  
Michael Wiggenhorn ◽  
Wolfgang Friess ◽  
Gerhard Winter ◽  
...  
Keyword(s):  
Silicone Oil ◽  
Mass Measurement ◽  
Flow Imaging ◽  
Oil Droplets ◽  
Complementary Methods ◽  
Micro Flow ◽  
Protein Particles

Unmasking Translucent Protein Particles by Improved Micro-Flow Imaging™ Algorithms

2014 ◽  
Vol 103 (1) ◽  
pp. 107-114 ◽  
Author(s):  
Jesper Søndergaard Pedersen ◽  
Malin Persson
Keyword(s):  
Flow Imaging ◽  
Micro Flow ◽  
Protein Particles

scholarly journals Value of Assessing Peripheral Vascularization with Micro-Flow Imaging, Resistive Index and Absent Hilum Sign as Predictor for Malignancy in Lymph Nodes in Head and Neck Squamous Cell Carcinoma

Cancers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 5071
Author(s):  
Petra K. de Koekkoek-Doll ◽  
Sander Roberti ◽  
Michiel W. van den Brekel ◽  
Monique Maas ◽  
Laura Smit ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Head And Neck ◽  
Squamous Cell ◽  
Predictive Value ◽  
Cell Cancer ◽  
Resistive Index ◽  
Needle Aspiration ◽  
Flow Imaging ◽  
Micro Flow ◽  
Additional Value

Ultrasound-guided fine needle aspiration cytology (USgFNAC) is commonly used for nodal staging in head and neck squamous cell cancer (HNSCC). Peripheral vascularity is a described feature for node metastasis. Micro-flow imaging (MFI) is a new sensitive technique to evaluate micro-vascularization. Our goal is to assess the additional value of MFI to detect malignancy in lymph nodes. A total of 102 patients with HNSCC were included prospectively. USgFNAC was performed with the Philips eL18–4 transducer. Cytological results served as a reference standard to evaluate the prediction of cytological malignancy depending on ultrasound features such as resistive index (RI), absence of fatty hilum sign, and peripheral vascularization. Results were obtained for all US examinations and for the subgroup of clinically node-negative neck (cN0). USgFNAC was performed in 211 nodes. Peripheral vascularization had a positive predictive value (PPV) of 83% (cN0: 50%) and the absence of a fatty hilum had a PPV of 82% (cN0 50%) The combination of peripheral vascularization and absent fatty hilum had a PPV of 94% (cN0: 72%). RI (threshold: 0.705) had a PPV of 61% (cN0: RI-threshold 0.615, PPV 20%), whereas the PPV of short axis diameter (threshold of 6.5mm) was 59% for all patients and 19% in cN0 necks (threshold of 4 mm). Peripheral vascularization assessed by MFI and absent hilum has a high predictive value for cytological malignancy in neck metastases. Next to size, both features should be used as additional selection criteria for USgFNAC.

Analysis of Cell Aggregation In Thawed Hematopoietic Stem Cell Products Using Micro-Flow Imaging.

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 1187-1187
Author(s):  
Luke Wu ◽  
Teresa Martin ◽  
Michael Halpenny ◽  
Antonio Giulivi ◽  
David S. Allan
Keyword(s):  
Stem Cell ◽  
Hematopoietic Stem Cell ◽  
Mononuclear Cells ◽  
Cell Aggregation ◽  
Dnase I ◽  
Flow Imaging ◽  
Minimal Cell ◽  
Hematopoietic Stem ◽  
Micro Flow ◽  
Cellular Aggregates

Abstract Abstract 1187 Background: Cell aggregation can be observed after thawing cryopreserved hematopoietic stem cell products used in transplantation. Micro-flow imaging (MFI) is a novel method of imaging biologic fluids and provides a means of quantifying cell aggregation. Method: Umbilical cord blood (UCB) was collected following informed consent and cryopreserved in 5% DMSO and 37% (w/v) pentastarch in liquid nitrogen. Aliquots of cryopreserved peripheral blood progenitor cell (PBPC) products were cryopreserved under similar conditions. Cells were thawed rapidly and filtered before analysis using MFI. Result: Large cellular aggregates were identified in all thawed PBPC samples analyzed (n=4) with a mean reduction in total cells of 19.7 ± 9.8% at 1 hour and 49.8 ± 15.9% at 24 hours. Cellular aggregates ≤ 170 um were visualized, sized and digitally imaged with MFI. Analysis of larger clumps was limited due to size of the analysis chamber. Cell transparency and circularity were unchanged over 24h after thawing and similar to freshly isolated PBPCs. Mononuclear cell yields did not diminish over 24 hours in freshly isolated PBPC (n=3, p>0.05) or in fresh UCB (n=3, p>0.05). Thawed UCB samples did not demonstrate significant clumping and maintained higher yields of total cells compared with PBPCs (p=0.02). Filtering of thawed PBPC products using standard blood transfusion filters (Baxter, 170 um) did not change the qualitative nature of observed cellular aggregates. The loss of cells in PBPC products correlated with early apoptotic mononuclear cells (7AAD-AnnV+). Treatment with DNase I eliminated the presence of observable cell clumping in thawed PBPCs at 24h with a marked improvement in cell yield at 24h (p<0.0001). Conclusion: Micro-flow imaging provides novel insight regarding cellular aggregation of blood stem cell products. Significant cell clumping occurs in cryopreserved PBPC after thawing and aggregates are not removed by standard transfusion filters. Aggregation increases with time after thawing and may influence the final dose of cells available for engraftment after transplantation. Fresh PBPC collections contain few aggregates and both fresh and thawed cryopreserved UCB demonstrated minimal cell clumping. Apoptosis induced by cryopreservation and thawing may contribute to increased aggregation in PBPCs and DNase I appears to preserve higher yields of total cells. New cryoprotectants that limit apoptosis are needed and methods to reduce cell clumping appear warranted. Disclosures: Martin: Brightwell Technologies: Employment.

Evaluation of the Vascular Architecture of Focal Liver Lesions Using Micro Flow Imaging

2013 ◽  
Vol 32 (7) ◽  
pp. 1157-1171 ◽  
Author(s):  
Hong Yang ◽  
Guang-Jian Liu ◽  
Ming-De Lu ◽  
Hui-Xiong Xu ◽  
Xiao-Yan Xie
Keyword(s):  
Focal Liver Lesions ◽  
Flow Imaging ◽  
Liver Lesions ◽  
Vascular Architecture ◽  
Micro Flow

scholarly journals Calcium Chloride in Neonatal Parenteral Nutrition Solutions with and without Added Cysteine: Compatibility Studies Using Laser and Micro-Flow Imaging Methodology

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0136894 ◽  
Author(s):  
Robert K. Huston ◽  
J. Mark Christensen ◽  
Sultan M. Alshahrani ◽  
Sumeia M. Mohamed ◽  
Sara M. Clark ◽  
...  
Keyword(s):  
Parenteral Nutrition ◽  
Calcium Chloride ◽  
Flow Imaging ◽  
Compatibility Studies ◽  
Micro Flow ◽  
Imaging Methodology

Quantification and Characterization of Subvisible Proteinaceous Particles in Opalescent mAb Formulations Using Micro-Flow Imaging

2010 ◽  
Vol 99 (6) ◽  
pp. 2628-2642 ◽  
Author(s):  
Deepak K. Sharma ◽  
Peter Oma ◽  
Mark J. Pollo ◽  
Muppalla Sukumar
Keyword(s):  
Flow Imaging ◽  
Micro Flow ◽  
Proteinaceous Particles
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