In vitro percutaneous absorption and in vivo protoporphyrin IX accumulation in skin and tumors after topical 5-aminolevulinic acid application with enhancement using an erbium:YAG laser

2006 ◽  
Vol 95 (4) ◽  
pp. 929-938 ◽  
Author(s):  
Shing-Chuan Shen ◽  
Woan-Ruoh Lee ◽  
Yi-Ping Fang ◽  
Chung-Hong Hu ◽  
Jia-You Fang
Keyword(s):  
Percutaneous Absorption ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Erbium:Yag Laser ◽  
In Vitro Percutaneous Absorption

In vitro and in vivo effects of HAL on porphyrin production in rat bladder cancer cells (AY27)

2019 ◽  
Vol 23 (07n08) ◽  
pp. 813-820
Author(s):  
Odrun A. Gederaas ◽  
Harald Husebye ◽  
Anders Johnsson ◽  
Susan Callaghan ◽  
Anders Brunsvik
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Active Agent ◽  
Laser Scanning Microscopy ◽  
Rat Bladder ◽  
Bladder Cancer Cells

Aminolevulinic acid and hexyl-aminolevulinate serve as biological precursors to produce photosensitive porphyrins in cells via the heme biosynthetic pathway. This pathway is integral to porphyrin-based photodynamic diagnosis and therapy. By adding exogenous hexyl-aminolevulinate to rat bladder cancer cells (AY27, in vitro) and an animal bladder cancer model (in vivo), fluorescent endogenous porphyrin production was stimulated. Lipophilic protoporphyrin IX was identified as the dominant species by reverse high-pressure liquid chromatography. Subcellular porphyrin localization in the AY27 cells was evaluated by confocal laser scanning microscopy and showed almost quantitative bleaching after 20 s. From this study, we ascertained that the protocol described herein is suitable for hexyl-aminolevulinate-mediated photodynamic therapy and diagnosis when protoporphyrin IX is the active agent.

Methods for in vitro percutaneous absorption studies I. Comparison with in vivo results

1982 ◽  
Vol 62 (3) ◽  
pp. 474-480 ◽  
Author(s):  
Robert L. Bronaugh ◽  
Raymond F. Stewart ◽  
Elaine R. Congdon ◽  
Albert L. Giles
Keyword(s):  
Percutaneous Absorption ◽  
Absorption Studies ◽  
In Vitro Percutaneous Absorption

In vivo and in vitro percutaneous absorption of [14C]pyrene in Sprague Dawley male rats: skin reservoir effect and consequence on urinary 1-OH pyrene excretion

2008 ◽  
Vol 82 (10) ◽  
pp. 739-747 ◽  
Author(s):  
Jean-Paul Payan ◽  
Michel Lafontaine ◽  
Patrice Simon ◽  
Fabrice Marquet ◽  
Catherine Champmartin-Gendre ◽  
...  
Keyword(s):  
Percutaneous Absorption ◽  
Male Rats ◽  
Sprague Dawley ◽  
Reservoir Effect ◽  
Skin Reservoir ◽  
In Vitro Percutaneous Absorption

scholarly journals Correction to: Predicting in vivo absorption of chloramphenicol in frogs using in vitro percutaneous absorption data

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Victoria K. Llewelyn ◽  
Lee Berger ◽  
Beverley D. Glass
Keyword(s):  
Percutaneous Absorption ◽  
Absorption Data ◽  
In Vivo Absorption ◽  
In Vitro Percutaneous Absorption

An amendment to this paper has been published and can be accessed via the original article.

In vitro and in vivo expression of protoporphyrin IX induced by lipophilic 5-aminolevulinic acid derivatives

2001 ◽  
Vol 27 (2) ◽  
pp. 114-120 ◽  
Author(s):  
Yoshiharu Ninomiya ◽  
Yoshiyasu Itoh ◽  
Shingo Tajima ◽  
Akira Ishibashi
Keyword(s):  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
In Vivo Expression
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