Determination of drug plasma protein binding by solid phase microextraction

2006 ◽  
Vol 95 (8) ◽  
pp. 1712-1722 ◽  
Author(s):  
Florin Marcel Musteata ◽  
Janusz Pawliszyn ◽  
Mark G. Qian ◽  
Jing-Tao Wu ◽  
Gerald T. Miwa
Keyword(s):  
Protein Binding ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Drug Plasma

Solid-phase microextraction for assessment of plasma protein binding, a complement to rapid equilibrium dialysis

Bioanalysis ◽  
2021 ◽  
Author(s):  
Sheelan Ahmad ◽  
Daniel Baker ◽  
Darragh Murnane ◽  
Neil Spooner ◽  
Ute Gerhard
Keyword(s):  
Protein Binding ◽  
Plasma Protein Binding ◽  
Plasma Protein ◽  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Equilibrium Dialysis ◽  
Free Concentration ◽  
Rapid Equilibrium

Aim: Determination of plasma protein binding ( PPB) is considered vital for better understanding of pharmacokinetic and pharmacodynamic activities of drugs due to the role of free concentration in pharmacological response. Methodology & results: Solid-phase microextraction (SPME) was investigated for measurement of PPB from biological matrices and compared with a gold standard approach (rapid equilibrium dialysis [RED]). Discussion & conclusion: SPME-derived values of PPB correlated well with literature values, and those determined by RED. Respectively, average protein binding across three concentrations by RED and SPME was 33.1 and 31.7% for metoprolol, 89.0 and 86.6% for propranolol and 99.2 and 99.0% for diclofenac. This study generates some evidence for SPME as an alternative platform for the determination of PPB.

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