Preparation and Crystal Characterization of a Polymorph, a Monohydrate, and an Ethyl Acetate Solvate of the Antifungal Fluconazole

2004 ◽  
Vol 93 (3) ◽  
pp. 601-611 ◽  
Author(s):  
Mino R. Caira ◽  
Khouloud A. Alkhamis ◽  
Rana M. Obaidat
Keyword(s):  
Ethyl Acetate ◽  
Crystal Characterization

scholarly journals Karakterisasi Fraksi Aktif Antioksidan dari Ekstrak Etanol Biji Kemangi (Ocimum Basilicum L.)

2015 ◽  
pp. 39-49
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Chitta Putri Novianti
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Solvent Fractionation ◽  
Butanol Extract ◽  
Dpph Method ◽  
Ic50 Values

Characterization of antioxidant compounds from the seeds of basil (Ocimum basilicum L.) has been done. Extraction is done by maceration method using ethanol solvent, fractionation by TLC and column chromatography, antioxidants test using DPPH method, and characterization of antioxidant compound using GCMS. Ethanol extract and results of fractionation ethanol extract of basil seeds using n-hexane, ethyl acetate, n-butanol and methanol-water extracts show that n-butanol extract has the highest antioxidant activity with IC50 values ​​of 41.90 ppm. Results of column chromatography n-butanol extract using n-hexane : ethyl acetate (1:9) as mobile phase yielded 5 fractions with fraction 4 (F4 isolate) has dominant stain of active antioxidants after being sprayed DPPH reagent, it had IC50 values ​​of 39,70 ppm and total phenolic content of 0,003 mg/g. Isolate F4 suspected contains two active compounds as antioxidant which is terpenoid and phenolic compound group, namely squalene and 1,4-di-tert-buthyl-phenol identified by GCMS.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3598

scholarly journals Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

2017 ◽  
Vol 3 (1) ◽  
pp. 79-87
Author(s):  
Alfindah Rusanti ◽  
Dede Sukandar ◽  
Tarso Rudiana ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Cytotoxic Compounds ◽  
Murine Leukemia ◽  
Etlingera Elatior

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

Crystal Characterization of Spherical Silicon Solar Cell by X-ray Diffraction

2006 ◽  
Vol 45 (5A) ◽  
pp. 3933-3937 ◽  
Author(s):  
Satoshi Omae ◽  
Takashi Minemoto ◽  
Mikio Murozono ◽  
Hideyuki Takakura ◽  
Yoshihiro Hamakawa
Keyword(s):  
Solar Cell ◽  
Silicon Solar Cell ◽  
X Ray Diffraction ◽  
X Ray ◽  
Crystal Characterization

Influence of the crystal characterization of CsI thin film for x-ray image detectors

2008 ◽  
Author(s):  
Shuang Liu ◽  
Xu Chen ◽  
Zhiyong Zhong ◽  
Charles M. Falco
Keyword(s):  
Thin Film ◽  
X Ray ◽  
Crystal Characterization

scholarly journals BIOLOGICAL CONTROL OF PHYTOPATHOGENIC FUNGI USING DIFFERENT EXTRACTS OF CHAETOMIUM CUPREUM

2018 ◽  
Vol 11 (9) ◽  
pp. 328
Author(s):  
Sharmila Tirumale ◽  
Nazir Wani
Keyword(s):  
Ethyl Acetate ◽  
Mycelial Growth ◽  
Ethyl Acetate Extract ◽  
Pathogenic Fungi ◽  
Phytopathogenic Fungi ◽  
Plant Pathogenic Fungi ◽  
Purification And Characterization ◽  
Butanol Extract ◽  
Ethyl Acetate Extracts

Objective: This study evaluated the Chaetomium cupreum extracts as biocontrol agents against four plant pathogenic fungi (Cladosporium cladosporioides, Fusarium oxysporum, Phomopsis azadirachtae, and Rhizoctonia solani).Method: The antifungal activity of n-butanol and ethyl acetate extracts of C. cupreum was evaluated against plant pathogenic fungi using food poison method.Result: In n-butanol extract, the percentage inhibition of mycelial growth against C. cladosporoides was 88.3±0.1, F. oxysporum was 59.4±0.2, R. solani was 56.2±0.9, and P. azadirachtae was 52.0±0.1at 0.25 mg/ml, respectively. In ethyl acetate extract, the percentage inhibition of mycelial growth against C. cladosporoides was 86.0±0.5, F. oxysporum was 66.4±0.1, P. azadirachtae was 55.2±0.9, and R. solani was 52.0±0.1 at 0.25 mg/ml, respectively.Conclusion: It was found that n-butanol extract is more effective than ethyl acetate extract of C. cupreum. Future studies will focus on the purification and characterization of compounds of C. cupreum and their biocontrol capacity with the mechanism for plant pathological applications.

Production and Characterization of Antibody Against PR Toxin

1988 ◽  
Vol 51 (6) ◽  
pp. 463-466 ◽  
Author(s):  
RU-DONG WEI ◽  
FUN S. CHU
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Ethyl Acetate ◽  
Bovine Serum ◽  
Antibody Specificity ◽  
Practical Application ◽  
Reductive Alkylation

Antibody against PR toxin was produced after immunizing rabbits with an immunogen prepared by conjugation of PR toxin to bovine serum albumin by a reductive alkylation method. A competitive radioimmunoassay (RIA) was used to determine the antibody specificity. The concentration causing 50% inhibition of binding of 3H-tetrahydro-PR toxin to the antibody by unlabeled PR toxin, tetrahydro-PR toxin, PR imine, eremofortin C (EC), acetyl-EC (Ac-EC), eremofortin D (ED) and eremofortin A (EA) were 7, 10, 5, 15, 50, 500 and 800 ng/assay, respectively; for PR alcohol and eremofortin B (EB), the concentration was greater than 10,000 ng/assay. The practical application of using this antibody for RIA of PR toxin was tested by spiking cheese with the toxin. PR toxin was then extracted with ethyl acetate, and analyzed by RIA. The overall recovery for 20 samples with 0.1 to 50 ppm of PR toxin was 93%.

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