Effect of interleukin‐22 on osteogenic differentiation and the osteoclastogenic response of human periodontal ligament fibroblasts in vitro

2020 ◽  
Vol 91 (8) ◽  
pp. 1085-1097
Author(s):  
Chuanjiang Zhao ◽  
Qianying Chen ◽  
Shaojie Yu ◽  
Chenrong Xu ◽  
Xiting Li ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Periodontal Ligament ◽  
Periodontal Ligament Fibroblasts ◽  
Interleukin 22 ◽  
Human Periodontal Ligament Fibroblasts

scholarly journals In Vitro Compression Model for Orthodontic Tooth Movement Modulates Human Periodontal Ligament Fibroblast Proliferation, Apoptosis and Cell Cycle

Biomolecules ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 932
Author(s):  
Julia Brockhaus ◽  
Rogerio B. Craveiro ◽  
Irma Azraq ◽  
Christian Niederau ◽  
Sarah K. Schröder ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Death ◽  
Periodontal Ligament ◽  
Environmental Changes ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Compressive Force ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts

Human Periodontal Ligament Fibroblasts (hPDLF), as part of the periodontal apparatus, modulate inflammation, regeneration and bone remodeling. Interferences are clinically manifested as attachment loss, tooth loosening and root resorption. During orthodontic tooth movement (OTM), remodeling and adaptation of the periodontium is required in order to enable tooth movement. hPDLF involvement in the early phase-OTM compression side was investigated for a 72-h period through a well-studied in vitro model. Changes in the morphology, cell proliferation and cell death were analyzed. Specific markers of the cell cycle were investigated by RT-qPCR and Western blot. The study showed that the morphology of hPDLF changes towards more unstructured, unsorted filaments under mechanical compression. The total cell numbers were significantly reduced with a higher cell death rate over the whole observation period. hPDLF started to recover to pretreatment conditions after 48 h. Furthermore, key molecules involved in the cell cycle were significantly reduced under compressive force at the gene expression and protein levels. These findings revealed important information for a better understanding of the preservation and remodeling processes within the periodontium through Periodontal Ligament Fibroblasts during orthodontic tooth movement. OTM initially decelerates the hPDLF cell cycle and proliferation. After adapting to environmental changes, human Periodontal Ligament Fibroblasts can regain homeostasis of the periodontium, affecting its reorganization.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

2006 ◽  
Vol 7 (1) ◽  
pp. 35-43 ◽  
Author(s):  
R. Viswa Chandra ◽  
Ganesh Chandra Jagetia ◽  
K. Mahalinga Bhat
Keyword(s):  
Citric Acid ◽  
Periodontal Ligament ◽  
Chinese Hamster ◽  
Lung Fibroblasts ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Root Surfaces ◽  
Tetracycline Hcl

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

Gene Profiling in Human Periodontal Ligament Fibroblasts by Subtractive Hybridization

2003 ◽  
Vol 82 (8) ◽  
pp. 641-645 ◽  
Author(s):  
T. Yamamoto ◽  
F. Myokai ◽  
F. Nishimura ◽  
T. Ohira ◽  
N. Shiomi ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Periodontal Ligament ◽  
Subtractive Hybridization ◽  
Gene Profiling ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts

Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 103 pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.

The effects of chitosan on the human periodontal ligament fibroblasts in vitro

2001 ◽  
Vol 31 (4) ◽  
pp. 823 ◽  
Author(s):  
Jeong-Won Paik ◽  
Hyun-Jung Lee ◽  
Yun-Jung Yoo ◽  
Kyoo-Sung Cho ◽  
Chong-Kwan Kim ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts
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