Synovial and Cartilage Responsiveness to Peri‐Operative Hyaluronic Acid +/‐ Dexamethasone Administration Following a Limited Injury to the Rabbit Stifle Joint

2021 ◽  
Author(s):  
BJ Heard ◽  
KI Barton ◽  
S Abubacker ◽  
M Chung ◽  
CR Martin ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Stifle Joint ◽  
And Cartilage

scholarly journals Rheumatoid Arthritis: Hyaluronic Acid and Cartilage Oligomeric Matrix Protein as Predictors of the Disease Progression

2016 ◽  
Vol 9 (1) ◽  
pp. 15-23
Author(s):  
SAID AL-DALAEN ◽  
AIMAN AL-QTAITAT ◽  
MOHAMMAD AL-RAWASHDEH ◽  
JIHAD ALZYOUD ◽  
AIMAN AL-MAATHADI
Keyword(s):  
Rheumatoid Arthritis ◽  
Hyaluronic Acid ◽  
Disease Progression ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
And Cartilage

scholarly journals Comparison of Enzymatic Hydrolysis of Polysaccharides from Eggshells Membranes

2016 ◽  
Vol 15 (2) ◽  
pp. 133-141 ◽  
Author(s):  
Eva Ürgeová ◽  
Katarína Vulganová
Keyword(s):  
Extracellular Matrix ◽  
Hyaluronic Acid ◽  
Enzymatic Hydrolysis ◽  
Glucuronic Acid ◽  
Optimal Conditions ◽  
Osteoarthritis Treatment ◽  
Neural Tissues ◽  
Eggshell Membranes ◽  
Hydrolysis Of ◽  
And Cartilage

Abstract AHyaluronic acid (HA) is part of the extracellular matrix of connective, epithelial and neural tissues, as well as the synovial fluid, skin, and cartilage. It is composed of repeating disaccharide units of D-glucuronic acid and N-acetyl glucosamine. Hyaluronic acid is used in abdominal surgery, ophthalmology, dermatology, rhinology; it is usable for the osteoarthritis treatment. The membranes of eggshell are a natural source of hyaluronic acid, collagen, glycosaminoglycan and collagenous proteins. In paper, we tested the possibility of extraction hyaluronic acid from the eggshell membranes by enzymatic hydrolysis. We identified optimal conditions of hydrolysis with trypsin at reaction temperature of 37 °C and pH 8; with pepsin at 40 °C and pH 3, as well as with papain at 60 °C and pH 7.5. The content of hyaluronic acid in samples was determined spectrophotometrically using the carbazole method. The experimental results showed a yield of ~ 4 -4.5 % hyaluronic acid per 1 g of dry eggshell membranes.

scholarly journals A placebo-controlled study comparing the efficacy of intra-articular injections of hyaluronic acid and a novel hyaluronic acid-platelet-rich plasma conjugate in a canine model of osteoarthritis

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Mun-Ik Lee ◽  
Jun-Hyung Kim ◽  
Ho-Hyun Kwak ◽  
Heung-Myong Woo ◽  
Jeong-Hee Han ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Range Of Motion ◽  
Platelet Rich Plasma ◽  
Cruciate Ligament ◽  
Treated Group ◽  
Canine Model ◽  
Controlled Study ◽  
Radiographic Examination ◽  
Time Points ◽  
Stifle Joint

Abstract Background The objective of this study was to assess the efficacy of intra-articular injections of hyaluronic acid (HA) and a novel, on-site conjugate of HA with autologous fibrinogen in platelet-rich plasma (HA-PRP) in a canine model of osteoarthritis (OA) Methods Twelve beagle dogs underwent a unilateral resection of the cranial cruciate ligament (CrCL) of the stifle joint. Clinical and radiographic signs of OA were confirmed in all dogs 8 weeks following CrCL resection and prior to treatment. The dogs were randomized into three groups: saline (n = 4), HA (n = 4), and HA-PRP (n = 4). Each dog received intra-articular injections of the respective substance into the affected joint at pre-determined time points. The dogs were assessed for adverse effects for 3 days after each injection and for lameness, pain, range of motion, kinetics, and radiographic OA severity prior to treatment and 3 months after injection. OA severity as determined by radiographic examination was not significantly different among the groups at any time point. The dogs were then humanely euthanatized and the stifle joint assessed by gross and histological examinations. Results Dogs treated with four weekly injections of HA or two biweekly injections of HA-PRP were significantly (p < 0.05) better than dogs treated with four weekly injections of saline at 2-, 4-, and 12-week time points based on a comfortable range of motion (CROM) and clinical lameness score. Gait analysis measuring symmetry and weight distribution on pressure sensor walkway showed significantly (p < 0.05) improved limb function for dogs treated with HA and HA-PRP compared with dogs treated with saline yet with better clinical outcome for the HA-PRP-treated group at 12 and 20 weeks follow-up. Gross and histological analysis of synovium and articular cartilage demonstrated significant (p < 0.05) improvement by both treatments groups compared to controls. There was however significantly (p < 0.05) less damage to the cartilage in the HA-PRP group compared to the HA-treated group. Conclusions These data suggest that while injection of HA and HA-PRP may be sufficient for short-term amelioration of the symptoms associated with OA, treatment with HA-PRP conjugates may be superior, providing significantly better long-term cartilage preservation.

scholarly journals Synoviology: a new chapter entitled to joints care

2017 ◽  
Vol 8 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Beniamino Palmieri ◽  
Thierry Conrozier ◽  
Maria Vadalà ◽  
Carmen Laurino
Keyword(s):  
Hyaluronic Acid ◽  
Synovial Membrane ◽  
Therapeutic Options ◽  
Evidence Based ◽  
Medical Sciences ◽  
Medical Treatments ◽  
Disease Therapy ◽  
Quality And Reliability ◽  
The Impact ◽  
And Cartilage

In order to extensively investigate on the synovial membrane – related diseases, we outlined a specific medical branch named “Synoviology”, targeted to the physiopathology and therapy of synovial membrane dysfunction. We searched Pubmed/Medline using the terms “synovial disease”, “therapy”, “synovial membrane”, “joints” and “drugs”, alone and combined. Selected papers from 1960 to 2015 were chosen based on their content (evidence-based quality and reliability). Clinical and experimental articles were included. Viscosupplementation with structurally different hyaluronic acid compounds, for restoration of the synovial membrane, and cartilage. The impact of other old and new medical treatments either locally or systemically administered was also included. Synoviology integrates biological, clinical and biochemical info for the progress of new therapeutic options in osteo-articular pathology.Asian Journal of Medical Sciences Vol.8(3) 2017 1-10

scholarly journals Hyaluronic acid synthesis, degradation, and crosslinking in equine osteoarthritis: TNF-α-TSG-6-mediated HC-HA formation

2021 ◽  
Vol 23 (1) ◽  
Author(s):  
Diana C. Fasanello ◽  
Jin Su ◽  
Siyu Deng ◽  
Rose Yin ◽  
Marshall J. Colville ◽  
...  
Keyword(s):  
Gene Expression ◽  
Hyaluronic Acid ◽  
Synovial Fluid ◽  
Complex Formation ◽  
Synovial Membrane ◽  
Fluid Composition ◽  
Fluid Viscosity ◽  
Tnf Α ◽  
Ha Synthase ◽  
And Cartilage

Abstract Background TNF-α-stimulated gene 6 (TSG-6) protein, a TNF-α-responsive hyaladherin, possesses enzymatic activity that can catalyze covalent crosslinks of the polysaccharide hyaluronic acid (HA) to another protein to form heavy chain-hyaluronic acid (HC-HA) complexes in pathological conditions such as osteoarthritis (OA). Here, we examined HA synthase and inflammatory gene expression; synovial fluid HA, TNF-α, and viscosity; and TSG-6-mediated HC-HA complex formation in an equine OA model. The objectives of this study were to (1) evaluate the TNF-α-TSG-6-HC-HA signaling pathway across multiple joint tissues, including synovial membrane, cartilage, and synovial fluid, and (2) determine the impact of OA on synovial fluid composition and biophysical properties. Methods HA and inflammatory cytokine concentrations (TNF-α, IL-1β, CCL2, 3, 5, and 11) were analyzed in synovial fluid from 63 OA and 25 control joints, and HA synthase (HAS1-3), TSG-6, and hyaluronan-degrading enzyme (HYAL2, HEXA) gene expression was measured in synovial membrane and cartilage. HA molecular weight (MW) distributions were determined using agarose gel electrophoresis and solid-state nanopore measurements, and HC-HA complex formation was detected via immunoblotting and immunofluorescence. SEC-MALS was used to evaluate TSG-6-mediated HA crosslinking, and synovial fluid and HA solution viscosities were analyzed using multiple particle-tracking microrheology and microfluidic measurements, respectively. Results TNF-α concentrations were greater in OA synovial fluid, and TSG6 expression was upregulated in OA synovial membrane and cartilage. TSG-6-mediated HC-HA complex formation was greater in OA synovial fluid and tissues than controls, and HC-HA was localized to both synovial membrane and superficial zone chondrocytes in OA joints. SEC-MALS demonstrated macromolecular aggregation of low MW HA in the presence of TSG-6 and inter-α-inhibitor with concurrent increases in viscosity. Conclusions Synovial fluid TNF-α concentrations, synovial membrane and cartilage TSG6 gene expression, and HC-HA complex formation were increased in equine OA. Despite the ability of TSG-6 to induce macromolecular aggregation of low MW HA with resultant increases in the viscosity of low MW HA solutions in vitro, HA concentration was the primary determinant of synovial fluid viscosity rather than HA MW or HC-HA crosslinking. The TNF-α-TSG-6-HC-HA pathway may represent a potential therapeutic target in OA.

Dynamic Compressive Loading and Crosslinking Density Influence the Chondrogenic and Hypertrophic Differentiation of Human Mesenchymal Stem Cells Seeded in Hyaluronic Acid Hydrogels

2012 ◽  
Author(s):  
Liming Bian ◽  
Robert L. Mauck ◽  
Jason A. Burdick
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Compressive Loading ◽  
Crosslinking Density ◽  
Cartilage Matrix ◽  
Human Mscs ◽  
Parathyroid Hormone Related Protein ◽  
And Cartilage ◽  
Dynamic Compressive Loading

While hyaluronic acid (HA) hydrogels provide a stable 3D environment that is conducive to the chondrogenesis of mesenchymal stem cells (MSCs) in the presence of growth factors [1], the neocartilage that is formed remains inferior to native tissue, even after long culture durations. Additionally, MSCs eventually transit into a hypertrophic phenotype after chondrogenic induction, resulting in the calcification of the ECM after ectopic transplantation [2]. From a material design perspective, variation in the HA hydrogel scaffold crosslinking density via changes in the HA macromer concentration can influence chondrogenesis of MSCs and neocartilage formation [3]. Recent studies have also demonstrated that dynamic compression enhances the expression of chondrogenic markers and cartilage matrix synthesis by MSCs encapsulated in various hydrogels, including agarose [4], alginate [5] and fibrin [6]. Furthermore, mechanical signals also regulate growth plate and articular cartilage chondrocyte hypertrophy via the IHH-PTHrP (India hedgehog, Parathyroid hormone-related protein) pathway [7]. In contrast to biologically inert scaffold materials, HA is capable of interacting with cells (including MSCs) via cell surface receptors (CD44, CD54, and CD168) [8; 9]. Therefore the objectives of this study were to (i) evaluate the effects of both hydrogel crosslinking and dynamic compressive loading on (i) chondrogenesis and cartilage matrix production/distribution of human MSCs encapsulated in HA gels and (ii) hypertrophic differentiation of human MSCs using an in vitro MSC hypertrophy model [10].

Differences in Cartilage Formed Intramuscularly or in Joint Surface Defects by Syngeneic Rat Chondrocytes Isolated from the Articular-Epiphyseal Cartilage Complex

1993 ◽  
Vol 2 (6) ◽  
pp. 467-473 ◽  
Author(s):  
Stanislaw Moskalewski ◽  
Anna Hyc ◽  
Tomasz Grzela ◽  
Jacek Malejczyk
Keyword(s):  
Hyaluronic Acid ◽  
Surface Defects ◽  
Articular Chondrocytes ◽  
Inhibitory Effect ◽  
Epiphyseal Cartilage ◽  
Joint Surface ◽  
Deep Zone ◽  
Superficial Zone ◽  
Chondrocyte Hypertrophy ◽  
And Cartilage

Syngeneic rat chondrocytes isolated from the articular-epiphyseal cartilage complex were suspended in hyaluronic acid and transplanted intramuscularly or into joint surface defects. Transplants were fixed in ruthenium hexammonium trichloride and embedded in glycol methacrylate. In cartilage nodules produced intramuscularly, chondrocyte hypertrophy and matrix calcification were observed after 2 wk. Partial ossification occurred after 4 wk and the cartilage was almost completely replaced by an ossicle after 8 wk. Only small, dispersed groups of chondrocytes remained within the ossicle. In cartilage formed in joint surface defects a superficial and a deep zone were distinguished. Chondrocytes in the superficial zone did not hypertrophy and cartilage remained unossified. In the deep zone matrix calcification and bone formation occurred. These processes were, however, retarded in comparison with intramuscular transplants. Thus, either intraarticular environment exerted an inhibitory effect on chondrocyte hypertrophy and matrix calcification or articular chondrocytes present among transplanted cells accumulated close to the joint lumen and reconstructed normal articular cartilage.

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