Differential effects of anti‐RANKL monoclonal antibody and zoledronic acid on necrotic bone in a murine model of Staphylococcus aureus ‐induced osteomyelitis

2021 ◽  
Author(s):  
Hideyuki Kobayashi ◽  
Ryo Fujita ◽  
Shigeto Hiratsuka ◽  
Tomohiro Shimizu ◽  
Dai Sato ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Zoledronic Acid ◽  
Murine Model ◽  
Necrotic Bone ◽  
Differential Effects

Formalin-Fixed Staphylococcus aureus Particles Prevent Allergic Sensitization in a Murine Model of Type I Allergy

2007 ◽  
Vol 144 (3) ◽  
pp. 183-196 ◽  
Author(s):  
Karina Gisch ◽  
Nadine Gehrke ◽  
Matthias Bros ◽  
Christina Priesmeyer ◽  
Jürgen Knop ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Murine Model ◽  
Allergic Sensitization ◽  
Type I ◽  
Type I Allergy ◽  
Formalin Fixed

scholarly journals Differential effects of zoledronic acid and oestrogen on anti-cancer immunity

Bone Reports ◽  
2021 ◽  
Vol 14 ◽  
pp. 101024
Author(s):  
Chris George ◽  
Diane Lefley ◽  
Victor Canuas-Landero ◽  
Claudia Tulotta ◽  
Hannah Corness ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Differential Effects ◽  
Cancer Immunity ◽  
Anti Cancer

scholarly journals Demonstration of the preclinical correlate of protection for Staphylococcus aureus clumping factor A in a murine model of infection

Vaccine ◽  
2015 ◽  
Vol 33 (41) ◽  
pp. 5452-5457 ◽  
Author(s):  
Ingrid L. Scully ◽  
Yekaterina Timofeyeva ◽  
David Keeney ◽  
Yury V. Matsuka ◽  
Elena Severina ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Murine Model ◽  
Correlate Of Protection

scholarly journals Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody

2008 ◽  
Vol 15 (10) ◽  
pp. 1541-1546 ◽  
Author(s):  
S. Datta ◽  
M. E. Janes ◽  
J. G. Simonson
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Cell Suspension ◽  
Vibrio Parahaemolyticus ◽  
Vibrio Vulnificus ◽  
Immunomagnetic Separation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Selective Isolation ◽  
Flagellar Protein ◽  
Phosphate Buffered Saline

ABSTRACT Mice were immunized by injection of Vibrio parahaemolyticus ATCC 17802 polar flagellin in order to produce monoclonal antibodies (mAbs). mAbs were analyzed by anti-H enzyme-linked immunosorbent assay using V. parahaemolyticus polar flagellar cores. The mAb exhibiting the highest anti-H titer was coated onto Cowan I Staphylococcus aureus cells at a concentration of 75 μg/ml cell suspension and used for slide coagglutination. Of 41 isolates identified genetically as V. parahaemolyticus, 100% coagglutinated with the anti-H mAb within 30 s, and the mAb did not react with 30 isolates identified as Vibrio vulnificus. A strong coagglutination reaction with V. parahaemolyticus ATCC 17802 was still observed when the S. aureus cells were armed with as little as 15 μg of mAb/ml S. aureus cell suspension. At this concentration, the mAb cross-reacted with three other Vibrio species, suggesting that they share an identical H antigen or antigens. The anti-H mAb was then used to optimize an immunomagnetic separation protocol which exhibited from 35% to about 45% binding of 102 to 103 V. parahaemolyticus cells in phosphate-buffered saline. The mAb would be useful for the rapid and selective isolation, concentration, and detection of V. parahaemolyticus cells from environmental sources.

scholarly journals Role of Antibodies in Protection Elicited by Active Vaccination with Genetically Inactivated Alpha Hemolysin in a Mouse Model of Staphylococcus aureus Skin and Soft Tissue Infections

2014 ◽  
Vol 21 (5) ◽  
pp. 622-627 ◽  
Author(s):  
Christopher P. Mocca ◽  
Rebecca A. Brady ◽  
Drusilla L. Burns
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Murine Model ◽  
Bacterial Colonization ◽  
Passive Immunization ◽  
The United States ◽  
Soft Tissue Infections ◽  
Content Type ◽  
Alpha Hemolysin ◽  
Active Vaccination

ABSTRACTDue to the emergence of highly virulent community-associated methicillin-resistantStaphylococcus aureus(CA-MRSA) infections,S. aureushas become a major threat to public health. A majority of CA-MRSA skin and soft tissue infections in the United States are caused byS. aureusUSA300 strains that are known to produce high levels of alpha hemolysin (Hla). Therefore, vaccines that contain inactivated forms of this toxin are currently being developed. In this study, we sought to determine the immune mechanisms of protection for this antigen using a vaccine composed of a genetically inactivated form of Hla (HlaH35L). Using a murine model of skin and soft tissue infections (SSTI), we found that BALB/c mice were protected by vaccination with HlaH35L; however, Jh mice, which are deficient in mature B lymphocytes and lack IgM and IgG in their serum, were not protected. Passive immunization with anti-HlaH35L antibodies conferred protection against bacterial colonization. Moreover, we found a positive correlation between the total antibody concentration induced by active vaccination and reduced bacterial levels. Animals that developed detectable neutralizing antibody titers after active vaccination were significantly protected from infection. These data demonstrate that antibodies to Hla represent the major mechanism of protection afforded by active vaccination with inactivated Hla in this murine model of SSTI, and in this disease model, antibody levels correlate with protection. These results provide important information for the future development and evaluation ofS. aureusvaccines.

Differential effects of low and high dose folic acid on endothelial dysfunction in a murine model of mild hyperhomocysteinaemia

2006 ◽  
Vol 551 (1-3) ◽  
pp. 92-97 ◽  
Author(s):  
Zoe L. Clarke ◽  
Stuart J. Moat ◽  
Alastair L. Miller ◽  
Michael D. Randall ◽  
Malcolm J. Lewis ◽  
...  
Keyword(s):  
Folic Acid ◽  
Endothelial Dysfunction ◽  
Murine Model ◽  
High Dose ◽  
Differential Effects ◽  
Mild Hyperhomocysteinaemia

scholarly journals Monoclonal Antibody Targeting Staphylococcus aureus Surface Protein A (SasA) Protect Against Staphylococcus aureus Sepsis and Peritonitis in Mice

PLoS ONE ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. e0149460 ◽  
Author(s):  
Yilong Yang ◽  
Mengying Qian ◽  
Shaoqiong Yi ◽  
Shuling Liu ◽  
Bing Li ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Monoclonal Antibody ◽  
Protein A ◽  
Surface Protein ◽  
Antibody Targeting
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