Development of a murine model of ischemic osteonecrosis to study the effects of aging on bone repair

2021 ◽  
Author(s):  
Ryosuke Yamaguchi ◽  
Nobuhiro Kamiya ◽  
Gen Kuroyanagi ◽  
Yinshi Ren ◽  
Harry K.W. Kim
Keyword(s):  
Murine Model ◽  
Bone Repair ◽  
Effects Of Aging ◽  
Ischemic Osteonecrosis

Interleukin-6 deletion stimulates revascularization and new bone formation following ischemic osteonecrosis in a murine model

Bone ◽  
2018 ◽  
Vol 116 ◽  
pp. 221-231 ◽  
Author(s):  
Gen Kuroyanagi ◽  
Naga Suresh Adapala ◽  
Ryosuke Yamaguchi ◽  
Nobuhiro Kamiya ◽  
Zhuo Deng ◽  
...  
Keyword(s):  
Bone Formation ◽  
Murine Model ◽  
Interleukin 6 ◽  
New Bone Formation ◽  
Ischemic Osteonecrosis

scholarly journals Role of Cbl-PI3K Interaction during Skeletal Remodeling in a Murine Model of Bone Repair

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138194 ◽  
Author(s):  
Vanessa Scanlon ◽  
Do Yu Soung ◽  
Naga Suresh Adapala ◽  
Elise Morgan ◽  
Marc F. Hansen ◽  
...  
Keyword(s):  
Murine Model ◽  
Bone Repair

Abstract P120: Effect of Age on Hemodynamics, Heart Rate Variability, and Outcome in a Murine Model of Sepsis

Circulation ◽  
2008 ◽  
Vol 118 (suppl_18) ◽  
Author(s):  
Steven Hollenberg ◽  
Sergio Zanotti ◽  
Jad Skaf ◽  
Hady Lichaa ◽  
Anupam Gupta ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Heart Rate Variability ◽  
Murine Model ◽  
Cecal Ligation ◽  
Experimental Period ◽  
Incidence And Mortality ◽  
Entire Experiment ◽  
Effects Of Aging ◽  
Young Mice

Background: The incidence and mortality of sepsis increase with aging, but animal models often use young animals whose biological ages do not reflect patient populations. We compared old and young mice in a murine model of resuscitated sepsis. Methods: C57Bl/6 mice (Young 2–3 months, n=32; Old 15–20 months, n=24) were made septic by cecal ligation and puncture (CLP) and resuscitated with fluids and antibiotics q 6 hr; controls underwent sham ligation. Serial echocardiography using a 30MHz probe was performed under light isoflurane anesthesia for measurement of stroke volume (SV), fractional shortening (FS), and cardiac output (CO). Blood pressure was measured using implantable radiotelemeters. From the waveforms, heart rate volatility (% of 5 minute intervals with standard deviation < the lowest 5% of baseline), an index of heart rate variability less susceptible to artifact than other measures, was derived for the entire experiment. Results: After CLP, heart rate and blood pressure did not differ significantly between old and young mice. SV decreased early in both groups (old 54.4 to 25.6 μL, young 57.2 to 24.0 p<0.01 vs baseline), as did CO. With resuscitation, SV and CO improved in both groups (old 48.0 μL and 24.5 ml/min, young 44.0 μL and 20.7 ml/min). Old animals were larger than young animals, but normalized values (% change from baseline) for SV, CO, and LVEDV were similar after CLP. Old septic mice had more periods of low heart rate volatility than young septic mice or old controls (62% vs 37% vs 3% of the experimental period). Mortality tended to be higher in old than young mice (46% vs 22% at 72 hr, p=0.09, 54% vs 22% at 144 hr, p=0.06). Conclusion: In a clinically relevant model of murine sepsis, age did not have a significant impact on hemodynamics, but decreased heart rate variability was more prominent in aged mice, and age was associated with increased mortality. This suggests the potential for nonlinear hemodynamic parameters to provide insights into both the pathogenesis of disease and the effects of aging.

Effects of aging on axon terminals in the dentate molecular layer

1987 ◽  
Vol 45 ◽  
pp. 928-929
Author(s):  
K. Cullen-Dockstader ◽  
E. Fifkova
Keyword(s):  
Granule Cells ◽  
Molecular Layer ◽  
Age Groups ◽  
Long Term Potentiation ◽  
Male Rats ◽  
Perforant Path ◽  
Axon Terminals ◽  
Fischer 344 ◽  
Spatial Memory Deficit ◽  
Effects Of Aging

Normal aging results in a pronounced spatial memory deficit associated with a rapid decay of long-term potentiation at the synapses between the perforant path and spines in the medial and distal thirds of the dentate molecular layer (DML), suggesting the alteration of synaptic transmission in the dentate fascia. While the number of dentate granule cells remains unchanged, and there are no obvious pathological changes in these cells associated with increasing age, the density of their axospinous contacts has been shown to decrease. There are indications that the presynaptic element is affected by senescence before the postsynaptic element, yet little attention has been given to the fine structure of the remaining axon terminals. Therefore, we studied the axon terminals of the perforant path in the DML across three age groups.5 Male rats (Fischer 344) of each age group (3, 24 and 30 months), were perfused through the aorta.

Effects of Aging on the Subcortical Encoding of Stop Consonants

2020 ◽  
Vol 29 (3) ◽  
pp. 391-403
Author(s):  
Dania Rishiq ◽  
Ashley Harkrider ◽  
Cary Springer ◽  
Mark Hedrick
Keyword(s):  
Older Adults ◽  
Repeated Measures ◽  
Mixed Model ◽  
Auditory Brainstem ◽  
Auditory Brainstem Responses ◽  
Aging Effects ◽  
Formant Frequency ◽  
Place Of Articulation ◽  
Second Formant ◽  
Effects Of Aging

Purpose The main purpose of this study was to evaluate aging effects on the predominantly subcortical (brainstem) encoding of the second-formant frequency transition, an essential acoustic cue for perceiving place of articulation. Method Synthetic consonant–vowel syllables varying in second-formant onset frequency (i.e., /ba/, /da/, and /ga/ stimuli) were used to elicit speech-evoked auditory brainstem responses (speech-ABRs) in 16 young adults ( M age = 21 years) and 11 older adults ( M age = 59 years). Repeated-measures mixed-model analyses of variance were performed on the latencies and amplitudes of the speech-ABR peaks. Fixed factors were phoneme (repeated measures on three levels: /b/ vs. /d/ vs. /g/) and age (two levels: young vs. older). Results Speech-ABR differences were observed between the two groups (young vs. older adults). Specifically, older listeners showed generalized amplitude reductions for onset and major peaks. Significant Phoneme × Group interactions were not observed. Conclusions Results showed aging effects in speech-ABR amplitudes that may reflect diminished subcortical encoding of consonants in older listeners. These aging effects were not phoneme dependent as observed using the statistical methods of this study.

Anti-OX40ligand treatment as a novel therapeutic strategy in a murine model of colitis

2001 ◽  
Vol 120 (5) ◽  
pp. A685-A685
Author(s):  
B SINGH ◽  
V MALMSTROM ◽  
F POWRIE
Keyword(s):  
Murine Model ◽  
Therapeutic Strategy ◽  
Novel Therapeutic

404: Immune Mediated Survival Advantage and Primary Tumor Control of Cryoablation Compared to Nephrectomy in a Murine Model of Advanced Renal Cancer

2006 ◽  
Vol 175 (4S) ◽  
pp. 132-132 ◽  
Author(s):  
Sean P. Hedican ◽  
Eric R. Wilkinson ◽  
Thomas F. Warner ◽  
Fred T. Lee ◽  
Stephen Y. Nakada
Keyword(s):  
Renal Cancer ◽  
Murine Model ◽  
Primary Tumor ◽  
Survival Advantage ◽  
Tumor Control ◽  
Advanced Renal Cancer ◽  
Immune Mediated
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