Once‐weekly parathyroid hormone combined with ongoing long‐term alendronate treatment promotes osteoporotic fracture healing in ovariectomized rats

2020 ◽  
Author(s):  
Chenggui Zhang ◽  
Junxiong Zhu ◽  
Jialin Jia ◽  
Zhiyuan Guan ◽  
Tiantong Sun ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Fracture Healing ◽  
Osteoporotic Fracture ◽  
Ovariectomized Rats ◽  
Alendronate Treatment

scholarly journals Biomechanical Characteristics of Osteoporotic Fracture Healing in Ovariectomized Rats: A Systematic Review

PLoS ONE ◽  
2016 ◽  
Vol 11 (4) ◽  
pp. e0153120 ◽  
Author(s):  
Lin Chen ◽  
Long Yang ◽  
Min Yao ◽  
Xue-Jun Cui ◽  
Chun-Chun Xue ◽  
...  
Keyword(s):  
Systematic Review ◽  
Fracture Healing ◽  
Osteoporotic Fracture ◽  
Ovariectomized Rats

scholarly journals Long-term effects of alendronate on fracture healing and bone remodeling of femoral shaft in ovariectomized rats

2013 ◽  
Vol 34 (3) ◽  
pp. 387-392 ◽  
Author(s):  
Ling-jie Fu ◽  
Ting-ting Tang ◽  
Yong-qiang Hao ◽  
Ke-rong Dai
Keyword(s):  
Fracture Healing ◽  
Bone Remodeling ◽  
Femoral Shaft ◽  
Ovariectomized Rats ◽  
Long Term Effects

Parathyroid hormone (1–34) promotes fracture healing in ovariectomized rats with type 2 diabetes mellitus

2017 ◽  
Vol 28 (10) ◽  
pp. 3043-3053 ◽  
Author(s):  
G. Y. Liu ◽  
G. L. Cao ◽  
F. M. Tian ◽  
H. P. Song ◽  
L. L. Yuan ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Parathyroid Hormone ◽  
Fracture Healing ◽  
Ovariectomized Rats

Effects of Parathyroid Hormone on Osteoporotic Fracture Healing in Mice via Non-Phospholipases C-Dependent Protein Kinase C Signaling Pathway

2021 ◽  
Vol 11 (5) ◽  
pp. 903-911
Author(s):  
Lei Wang ◽  
Linjuan Liu ◽  
Sixin Sun ◽  
Li Xiao ◽  
Qinyi Jiang ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Fracture Healing ◽  
Signaling Pathway ◽  
Osteoporotic Fracture ◽  
Expression Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Mineral Density ◽  
Pkc Inhibitor ◽  
Pkc Signaling

Objectives: This study was aimed to explore the effects of parathyroid hormone (PTH) on osteoporotic fracture healing in mice and the underlying mechanisms. Methods: Microarray analysis was conducted to analyze the gene expression level in MC3T3-E1 cells. Carboxyfluorescein succinimidyl ester (CFSE) staining and flow cytometry was adopted to analyze the proliferation and apopto-sis of MC3T3-E1 cells. qRT-PCR was used to analyze the mRNA expression level. Fluorescence resonance energy transfer (FRET) assay was conducted to detect PKC activity. The bone mineral density (BMD) and bone volume (BV)/total volume (TV) were determined via enzyme-linked immunosorbent assay (ELISA) and microscopic computed tomography (micro-CT). Results: ERK1/2 was abnormally expressed in MC3T3-E1 cells after GlylArg19hPTH (1-34) + KT5720 treatment. GlylArg19hPTH (1-34)+ KT5720 treatment promoted cell proliferation, inhibited cell apoptosis, and upregulatedthe expression of osteogenesis-related genes (ALP, OPN, Runx2 and OPG) in MC3T3-E1 cells, which were due to the activation of the non-PLC-dependent PKC signaling pathway and can be blocked by PKC inhibitor Go6983 or ERK1/2 inhibitor BVD-523. Moreover, the activity of PKC in MC3T3-E1 cells treated with GlylArg19hPTH (1-34) + KT5720 + Go6983 was alleviated by ERK1/2 inhibitor BVD-523. In vivo, specific activation of the non-PLC-dependent PKC signaling pathway increased the serum levels of APL and OPG in mice with osteoporotic fracture, which were reversed by PKC inhibitor Go6983 and ERK1/2 inhibitor BVD-523. Moreover, PKC inhibitor Go6983 and ERK1/2 inhibitor BVD-523 suppressed the elevation of BV/TV and BMD induced by specific activation of the non-PKC-dependent signaling pathway. Conclusions: Taken together, PTH stimulates osteoporotic fracture healing in mice through the non-PLC-dependent PKC signaling pathway in which ERK1/2 exerts a vital role.

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