Effect of Storage Conditions and Activation on Growth Factor Concentration in Platelet‐Rich Plasma

2019 ◽  
Vol 38 (4) ◽  
pp. 777-784 ◽  
Author(s):  
Joong Il Kim ◽  
Hyun Cheol Bae ◽  
Hee Jung Park ◽  
Myung Chul Lee ◽  
Hyuk Soo Han
Keyword(s):  
Growth Factor ◽  
Platelet Rich Plasma ◽  
Storage Conditions ◽  
Factor Concentration

scholarly journals Influence of storage conditions on the release of growth factors in platelet-rich blood derivatives

2016 ◽  
Vol 2 (1) ◽  
pp. 311-314 ◽  
Author(s):  
Katharina Düregger ◽  
Anqi Peng ◽  
Markus Eblenkamp
Keyword(s):  
Growth Factors ◽  
Growth Factor ◽  
Platelet Rich Plasma ◽  
Storage Conditions ◽  
Platelet Derived Growth Factor ◽  
Prolonged Storage ◽  
Wound Treatment ◽  
Time Dependency ◽  
Processing And Storage ◽  
And Storage

AbstractThrombocytes can be concentrated in blood derivatives and used as autologous transplants e.g. for wound treatment due to the release of growth factors such as platelet derived growth factor (PDGF). Conditions for processing and storage of these platelet-rich blood derivatives influence the release of PDGF from the platelet-bound α-granules into the plasma. In this study Platelet rich plasma (PRP) and Platelet concentrate (PC) were produced with a fully automated centrifugation system. Storage of PRP and PC for 1 h up to 4 months at temperatures between −20°C and +37°C was applied with the aim of evaluating the influence on the amount of released PDGF. Storage at −20°C resulted in the highest release of PDGF in PRP and a time dependency was determined: prolonged storage up to 1 month in PRP and 10 days in PC increased the release of PDGF. Regardless of the storage conditions, the release of PDGF per platelet was higher in PC than in PRP.

scholarly journals Freeze-Dried Human Platelet-Rich Plasma Retains Activation and Growth Factor Expression after an Eight-Week Preservation Period

2017 ◽  
Vol 11 (3) ◽  
pp. 329-336 ◽  
Author(s):  
Yasuhiro Shiga ◽  
Go Kubota ◽  
Sumihisa Orita ◽  
Kazuhide Inage ◽  
Hiroto Kamoda ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Growth Factors ◽  
Growth Factor ◽  
Freeze Drying ◽  
Platelet Rich Plasma ◽  
Storage Conditions ◽  
Healthy Human ◽  
Platelet Counts ◽  
Tissue Repair And Regeneration ◽  
Freeze Dried

<sec><title>Study Design</title><p>Controlled laboratory study.</p></sec><sec><title>Purpose</title><p>This study aimed to evaluate the efficacy of platelet-rich plasma (PRP) stored at room temperature (RT), frozen, or after freeze-drying.</p></sec><sec><title>Overview of Literature</title><p>PRP enriches tissue repair and regeneration, and is a novel treatment option for musculoskeletal pathologies. However, whether biological activity is preserved during PRP storage remains uncertain.</p></sec><sec><title>Methods</title><p>PRP was prepared from blood of 12 healthy human volunteers (200 mL/person) and stored using three methods: PRP was stored at RT with shaking, PRP was frozen and stored at −80℃, or PRP was freeze-dried and stored at RT. Platelet counts and growth factor content were examined immediately after preparation, as well as 2, 4, and 8 weeks after storage. Platelet activation rate was quantified by flow cytometry.</p></sec><sec><title>Results</title><p>Platelet counts were impossible to determine in many RT samples after 2 weeks, but they remained at constant levels in frozen and freeze-dried samples, even after 8 weeks of storage. Flow cytometry showed approximately 80% activation of the platelets regardless of storage conditions. Almost no growth factors were detected in the RT samples after 8 weeks, while low but significant expression was detected in the frozen and freeze-dried PRP. Over time, the mean relative concentrations of various growth factors decreased significantly or disappeared in the RT group. In the frozen group, levels were maintained for 4 weeks, but decreased significantly by 8 weeks (<italic>p</italic> &lt;0.05). The freeze-dried group maintained baseline levels of growth factors for the entire 8-week duration.</p></sec><sec><title>Conclusions</title><p>Freeze-drying enables PRP storage while maintaining bioactivity and efficacy for extended periods.</p></sec>

Implications of anticoagulants and gender on cell counts and growth factor concentration in platelet-rich plasma and platelet-rich gel supernatants from rabbits

2016 ◽  
Vol 29 (02) ◽  
pp. 115-124 ◽  
Author(s):  
Juan González ◽  
Catalina López ◽  
Jorge Carmona
Keyword(s):  
Growth Factor ◽  
Whole Blood ◽  
Sodium Citrate ◽  
Platelet Rich Plasma ◽  
Dextrose Solution ◽  
Cell Counts ◽  
Acid Citrate Dextrose ◽  
And Gender ◽  
Citrate Dextrose ◽  
Factor Concentration

SummaryObjectives: Our objectives were as follows: 1) to validate a protocol for producing rabbit platelet-rich plasma (PRP); 2) to determine the influence of two anticoagulants, sodium citrate and acid-citrate-dextrose solution A, and gender on cell count in PRP and growth factor concentration in pure platelet-rich gel supernatants; 3) to correlate the variables evaluated.Methods: Whole blood from 18 New Zealand rabbits (9 males and 9 females) was obtained with sodium citrate and acid- citrate-dextrose solution A for processing PRP fractions (A and B), which were evaluated for haematology. The PRP fractions were either activated with calcium gluconate or lysated with a detergent. The concentrations of transforming growth factor beta 1 and platelet-derived growth factor BB were assayed by ELISA.Results: The sodium citrate PRP-B had significantly higher counts of platelets in comparison to PRP-A and whole blood obtained with the same anticoagulant and the homologous acid-citrate-dextrose solution A PRP fraction. The sodium citrate PRP-A had a significantly higher count of leukocytes compared to the homologous acid-citrate-dextrose solution A fraction. All the PRP fractions had a significant leuko-reduction when compared to whole blood. The sodium citrate PRP-A fraction from female rabbits had significantly lower platelet counts and significantly higher leukocyte counts than the same acid-citrate-dextrose solution A fraction. Growth factor concentration was not affected by the type of anticoagulant or gender.Clinical significance: The type of anticoagulant and gender affected the cell counts in PRP, but they did not influence the growth factor concentration. More complete rabbit PRP studies should be performed before evaluating this type of substance in models of disease.

scholarly journals Effects of the breed, sex and age on cellular content and growth factor release from equine pure-platelet rich plasma and pure-platelet rich gel

2013 ◽  
Vol 9 (1) ◽  
pp. 29 ◽  
Author(s):  
Carlos E Giraldo ◽  
Catalina López ◽  
María E Álvarez ◽  
Ismael J Samudio ◽  
Marta Prades ◽  
...  
Keyword(s):  
Growth Factor ◽  
Platelet Rich Plasma ◽  
Cellular Content ◽  
Growth Factor Release ◽  
Factor Release

Platelet-rich plasma lyophilization enables growth factor preservation and functionality when compared with fresh platelet-rich plasma

2018 ◽  
Vol 13 (7) ◽  
pp. 775-784 ◽  
Author(s):  
Letícia Queiroz da Silva ◽  
Silmara Aparecida de Lima Montalvão ◽  
Amauri da Silva Justo-Junior ◽  
José Luiz Rosenberis Cunha Júnior ◽  
Stephany Cares Huber ◽  
...  
Keyword(s):  
Growth Factor ◽  
Platelet Rich Plasma
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