scholarly journals Sprifermin treatment enhances cartilage integration in an in vitro repair model

2018 ◽  
Vol 36 (10) ◽  
pp. 2648-2656 ◽  
Author(s):  
Mackenzie L. Sennett ◽  
Gregory R. Meloni ◽  
Alexandra J. E. Farran ◽  
Hans Guehring ◽  
Robert L. Mauck ◽  
...  
Keyword(s):  
In Vitro Repair ◽  
Cartilage Integration

In Vitro Repair of Meniscal Radial Tear With Hydrogels Seeded With Adipose Stem Cells and TGF-β3

2018 ◽  
Vol 46 (10) ◽  
pp. 2402-2413 ◽  
Author(s):  
Hiroshi Sasaki ◽  
Benjamin B. Rothrauff ◽  
Peter G. Alexander ◽  
Hang Lin ◽  
Riccardo Gottardi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Transforming Growth Factor ◽  
Meniscal Repair ◽  
Explant Culture ◽  
Differentiation Potential ◽  
3 Dimensional ◽  
Institutional Review ◽  
Radial Tear ◽  
In Vitro Repair

Background: Radial tears of the meniscus are a common knee injury, frequently resulting in osteoarthritis. To date, there are no established, effective treatments for radial tears. Adipose-derived stem cells (ASCs) may be an attractive cell source for meniscal regeneration because they can be quickly isolated in large number and are capable of undergoing induced fibrochondrogenic differentiation mediated by transforming growth factor β3 (TGF-β3). However, the use of ASCs for meniscal repair is largely unexplored. Hypothesis: ASC-seeded hydrogels with preloaded TGF-β3 will improve meniscal healing of radial tears, as modeled in an explant model. Study Design: Controlled laboratory study. Methods: With an institutional review board–exempted protocol, human ASCs were isolated from the infrapatellar fat pads of 3 donors, obtained after total knee replacement, and characterized. ASCs were encapsulated in photocrosslinkable methacrylated gelatin hydrogels to form 3-dimensional constructs, which were placed into tissue culture. The effect of TGF-β3—whether preloaded into the hydrogel or added as a soluble medium supplement—on matrix-sulfated proteoglycan deposition in the constructs was evaluated. A meniscal explant culture model was used to simulate meniscal repair. Cylindrical-shaped explants were excised from the inner avascular region of adult bovine menisci, and a radial tear was modeled by cutting perpendicular to the meniscal main fibers to the length of the radius. Six combinations of hydrogels—namely, acellular and ASC-seeded hydrogels supplemented with preloaded TGF-β3 (2 µg/mL) or soluble TGF-β3 (10 ng/mL) and without supplement—were injected into the radial tear and stabilized by photocrosslinking with visible light. At 4 and 8 weeks of culture, healing was assessed through histology, immunofluorescence staining, and mechanical testing. Results: ASCs isolated from the 3 donors exhibited colony-forming and multilineage differentiation potential. Hydrogels preloaded with TGF-β3 and those cultured in soluble TGF-β3 showed robust matrix-sulfated proteoglycan deposition. ASC-seeded hydrogels promoted superior healing as compared with acellular hydrogels, with preloaded or soluble TGF-β3 further improving histological scores and mechanical properties. Conclusion: These findings demonstrated that ASC-seeded hydrogels preloaded with TGF-β3 enhanced healing of radial meniscal tears in an in vitro meniscal repair model. Clinical Relevance: Injection delivery of ASCs in a TGF-β3-preloaded photocrosslinkable hydrogel represents a novel candidate strategy to repair meniscal radial tears and minimize further osteoarthritic joint degeneration.

scholarly journals Inhibition of Matrix Metalloproteinases Enhances In Vitro Repair of the Meniscus

2008 ◽  
Vol 467 (6) ◽  
pp. 1557-1567 ◽  
Author(s):  
Amy L. McNulty ◽  
J. Brice Weinberg ◽  
Farshid Guilak
Keyword(s):  
Matrix Metalloproteinases ◽  
In Vitro Repair

In vitro repair of radiation-induced strand breaks in DNA

1976 ◽  
Vol 432 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Luitgard Mitzel-Landbeck ◽  
Gisela Schutz ◽  
Ulrich Hagen
Keyword(s):  
Strand Breaks ◽  
Radiation Induced ◽  
In Vitro Repair

scholarly journals In vitro repair of DNA hairpins containing various numbers of CAG/CTG trinucleotide repeats

DNA Repair ◽  
2012 ◽  
Vol 11 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Tianyi Zhang ◽  
Jian Huang ◽  
Liya Gu ◽  
Guo-Min Li
Keyword(s):  
Trinucleotide Repeats ◽  
Dna Hairpins ◽  
In Vitro Repair

scholarly journals Developmental Defects and Male Sterility in Mice Lacking the Ubiquitin-Like DNA Repair Gene mHR23B

2002 ◽  
Vol 22 (4) ◽  
pp. 1233-1245 ◽  
Author(s):  
Jessica M. Y. Ng ◽  
Harry Vrieling ◽  
Kaoru Sugasawa ◽  
Marja P. Ooms ◽  
J. Anton Grootegoed ◽  
...  
Keyword(s):  
Male Sterility ◽  
Excision Repair ◽  
High Rate ◽  
Repair Gene ◽  
Developmental Defects ◽  
Mouse Mutants ◽  
Dna Repair Gene ◽  
Ubiquitin Proteasome ◽  
In Vitro Repair

ABSTRACT mHR23B encodes one of the two mammalian homologs of Saccharomyces cerevisiae RAD23, a ubiquitin-like fusion protein involved in nucleotide excision repair (NER). Part of mHR23B is complexed with the XPC protein, and this heterodimer functions as the main damage detector and initiator of global genome NER. While XPC defects exist in humans and mice, mutations for mHR23A and mHR23B are not known. Here, we present a mouse model for mHR23B. Unlike XPC-deficient cells, mHR23B−/− mouse embryonic fibroblasts are not UV sensitive and retain the repair characteristics of wild-type cells. In agreement with the results of in vitro repair studies, this indicates that mHR23A can functionally replace mHR23B in NER. Unexpectedly, mHR23B−/− mice show impaired embryonic development and a high rate (90%) of intrauterine or neonatal death. Surviving animals display a variety of abnormalities, including retarded growth, facial dysmorphology, and male sterility. Such abnormalities are not observed in XPC and other NER-deficient mouse mutants and point to a separate function of mHR23B in development. This function may involve regulation of protein stability via the ubiquitin/proteasome pathway and is not or only in part compensated for by mHR23A.

scholarly journals In Vitro Repair of UV-Irradiated Micrococcus luteus Bacteriophage N1 Transfecting DNA 1

1974 ◽  
Vol 13 (3) ◽  
pp. 765-767 ◽  
Author(s):  
Inga Mahler ◽  
Jeanne George ◽  
Lawrence Grossman
Keyword(s):  
Micrococcus Luteus ◽  
In Vitro Repair
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