scholarly journals Polydeoxyribonucleotide improves tendon healing following achilles tendon injury in rats

2017 ◽  
Vol 36 (6) ◽  
pp. 1767-1776 ◽  
Author(s):  
Shin Hyuk Kang ◽  
Min Seok Choi ◽  
Han Koo Kim ◽  
Woo Seob Kim ◽  
Tae Hui Bae ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Tendon Healing ◽  
Tendon Injury

The effect of tendon stem/progenitor cell (TSC) sheet on the early tendon healing in a rat Achilles tendon injury model

2016 ◽  
Vol 42 ◽  
pp. 136-146 ◽  
Author(s):  
Issei Komatsu ◽  
James H-C. Wang ◽  
Kiyotaka Iwasaki ◽  
Tatsuya Shimizu ◽  
Teruo Okano
Keyword(s):  
Achilles Tendon ◽  
Progenitor Cell ◽  
Tendon Healing ◽  
Tendon Injury ◽  
Injury Model

Analysis of Collagen Fiber Organization in Mouse Achilles Tendon Using High-Frequency Ultrasound Imaging

2013 ◽  
Author(s):  
Corinne N. Riggin ◽  
Joseph J. Sarver ◽  
Benjamin R. Freedman ◽  
Stephen J. Thomas ◽  
Louis J. Soslowsky
Keyword(s):  
Achilles Tendon ◽  
Functional Performance ◽  
Tendon Healing ◽  
Tendon Injury ◽  
High Frequency Ultrasound ◽  
Medical Expense ◽  
Collagen Organization ◽  
Tendon Ruptures ◽  
And Function

Achilles tendon ruptures are traumatic injuries that frequently occur in active individuals and result in significant medical expense. Common techniques for assessing outcomes of surgical repair and rehabilitation rely heavily on patient-based measures of pain and function. While these measures can provide evidence for recovery of functional performance, they do not directly assess tendon healing which, if insufficient, can lead to re-rupture. The clinical evaluation of collagen organization following Achilles tendon injury may provide a more accurate measure of healing than traditional, functional performance tests. It has been shown that changes in collagen organization precede and correlate with changes in mechanical properties in tendons [1–3] and that load and injury effect collagen organization [4–6]. Ultimately, if collagen organization could be quantified in vivo, it would represent a powerful, diagnostic tool to measure the progression of tendon healing, as well as to monitor damage accumulation due to injury.

scholarly journals Analysis of Collagen Organization in Mouse Achilles Tendon Using High-Frequency Ultrasound Imaging

2014 ◽  
Vol 136 (2) ◽  
Author(s):  
Corinne N. Riggin ◽  
Joseph J. Sarver ◽  
Benjamin R. Freedman ◽  
Stephen J. Thomas ◽  
Louis J. Soslowsky
Keyword(s):  
Achilles Tendon ◽  
Ultrasound Imaging ◽  
High Frequency ◽  
Tendon Healing ◽  
Tendon Injury ◽  
High Frequency Ultrasound ◽  
Collagen Organization ◽  
Collagen Alignment ◽  
Frequency Ultrasound

Achilles tendon ruptures are traumatic injuries, and techniques for assessing repair outcomes rely on patient-based measures of pain and function, which do not directly assess tendon healing. Consequently, there is a need for a quantitative, in vivo measure of tendon properties. Therefore, the purpose of this study was to validate ultrasound imaging for evaluating collagen organization in tendons. In this study, we compared our novel, high-frequency ultrasound (HFUS) imaging and analysis method to a standard measure of collagen organization, crossed polarizer (CP) imaging. Eighteen mouse Achilles tendons were harvested and placed into a testing fixture where HFUS and CP imaging could be performed simultaneously in a controlled loading environment. Two experiments were conducted: (1) effect of loading on collagen alignment and (2) effect of an excisional injury on collagen alignment. As expected, it was found that both the HFUS and CP methods could reliably detect an increase in alignment with increasing load, as well as a decrease in alignment with injury. This HFUS method demonstrates that structural measures of collagen organization in tendon can be determined through ultrasound imaging. This experiment also provides a mechanistic evaluation of tissue structure that could potentially be used to develop a targeted approach to aid in rehabilitation or monitor return to activity after tendon injury.

scholarly journals Effectiveness of Ozone Therapy on Tendon Healing: An Experimental Study in Generated Achilles Tendon Injury Model in Rats

2018 ◽  
Vol 27 (4) ◽  
pp. 309-314
Author(s):  
Volkan Kizilkaya ◽  
Vedat Uruc ◽  
Ali Levent ◽  
Ozgur Kanat ◽  
Mustafa Turgut Yildizgoren ◽  
...  
Keyword(s):  
Experimental Study ◽  
Achilles Tendon ◽  
Tendon Healing ◽  
Tendon Injury ◽  
Ozone Therapy ◽  
Injury Model

Effect of curcumin on tendon healing: an experimental study in a rat model of Achilles tendon injury

2018 ◽  
Vol 42 (8) ◽  
pp. 1905-1910 ◽  
Author(s):  
Ali Güleç ◽  
Yılmaz Türk ◽  
Bahattin Kerem Aydin ◽  
Ömer Faruk Erkoçak ◽  
Selim Safalı ◽  
...  
Keyword(s):  
Experimental Study ◽  
Achilles Tendon ◽  
Rat Model ◽  
Tendon Healing ◽  
Tendon Injury

scholarly journals Platelet-Rich Plasma Releasate Promotes Early Healing in Tendon After Acute Injury

2021 ◽  
Vol 9 (4) ◽  
pp. 232596712199037
Author(s):  
Tung-Yang Yu ◽  
Jong-Hwei S. Pang ◽  
Li-Ping Lin ◽  
Ju-Wen Cheng ◽  
Shih-Jung Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tensile Strength ◽  
Achilles Tendon ◽  
Platelet Rich Plasma ◽  
Testing Machine ◽  
Tendon Healing ◽  
Tendon Injury ◽  
The Other ◽  
Acute Injury ◽  
Early Healing

Background: Acute tendon injury can limit motion and thereby inhibit tendon healing. Positive results have been found after the use of platelet-rich plasma (PRP) to treat tendon injury; however, the early effects of PRP on tendon regeneration are not known. Purpose/Hypothesis: The purpose of this study was to evaluate the effects of PRP releasate (PRPr) on the early stages of tendon healing in a rat partial tenotomy model. It was hypothesized that PRPr can promote early healing of an Achilles tendon in rats. Study Design: Controlled laboratory study. Methods: PRP was prepared by a 2-step method of manual platelet concentration from 10 rats. PRPr was isolated from the clotted preparation after activation by thrombin and was applied to an Achilles tendon on 1 side of 30 rats on the second day after partial tenotomy, with normal saline used as the control on the other side. Achilles tendon samples were harvested 5 and 10 days after tenotomy. At each time point, 15 Achilles tendon samples were obtained, of which 5 samples were evaluated by Masson trichrome staining, apoptosis, and cell proliferation, while the other 10 samples were tested for tensile strength using a material testing machine. Results: Compared with saline-treated control tendons, the PRPr-treated tendons showed increased collagen synthesis near the cut edge and fewer apoptotic cells ( P = .01). An immunohistochemical analysis revealed more Ki-67–positive cells but fewer cluster of differentiation (CD) 68+ (ED1+) macrophages in PRPr tendons compared with saline-treated tendons ( P < .01). Tendons treated with PRPr also showed higher ultimate tensile strength than those treated with saline ( P = .03). Conclusion: PRPr treatment promotes tissue recovery in the early phase of tendon healing by stimulating tendon cell proliferation and collagen production while inhibiting cell apoptosis and CD68+ (ED1+) macrophage infiltration. Clinical Relevance: These findings suggest that with PRPr treatment, higher loads can be applied to the healing tendon at an earlier time, which can help the patient resume activity earlier.

Insulin-dependent diabetes impairs the inflammatory response and delays angiogenesis following Achilles tendon injury

2004 ◽  
Vol 286 (5) ◽  
pp. R952-R957 ◽  
Author(s):  
Nadia Chbinou ◽  
Jérôme Frenette
Keyword(s):  
Inflammatory Response ◽  
Achilles Tendon ◽  
Healing Process ◽  
Tendon Healing ◽  
Inflammatory Cells ◽  
Tendon Injury ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Impaired Wound Healing ◽  
The Core

Although impaired wound healing associated with type 1 diabetes mellitus has been well studied in skin tissue, the influence of this metabolic disorder on tendon healing and recovery has not been extensively investigated. Because tendons are known to have limited repair potential, we studied the tendon-healing process by using a diabetic rat tendonitis model. We tested the hypothesis that diabetes influences the inflammatory response, cell proliferation, and angiogenesis in injured Achilles tendons. Diabetes was induced by injecting streptozotocin at 45 mg/kg body wt. Non-diabetic rats as well as diabetic and insulin-treated diabetic animals were then injected with collagenase. The accumulation of inflammatory cells was quantified in transversal sections of Achilles tendon by using immunohistochemical staining at days 0, 1, 3, 7, 14, and 28 posttrauma. The number of proliferative cells and the extent of neovascularization was also quantified in the paratenon and the core of the tendon at days 0, 3, 7, 14, and 28 posttrauma. Relative to nondiabetic and insulin-treated diabetic animals, the numbers of accumulated neutrophils and ED1+ and ED2+ macrophages in diabetic rats decreased by 46, 43, and 52%, respectively, in the first 3 days after injury compared with levels in nondiabetic and insulin-treated diabetic animals. The density of newly formed blood vessels decreased by 35 and 29% in the paratenon and the core of tendon, respectively, at days 3 and 7 after injury. Lastly, the concentration of proliferative cells decreased by 34% in the paratenon at day 7 posttrauma in injured tendons from diabetic rats relative to nondiabetic rats. These results indicate that alterations in inflammatory, angiogenic, and proliferative processes occurred in the diabetic state that might eventually perturb tendon healing and remodeling.

Gender differences in the Achilles tendon load during the fencing lunge

2014 ◽  
Vol 6 (3) ◽  
Author(s):  
Jonathan Kenneth Sinclair ◽  
Lindsay Bottoms
Keyword(s):  
Gender Differences ◽  
Achilles Tendon ◽  
Motion Capture ◽  
Tendon Injury ◽  
Force Platform ◽  
Motion Capture System ◽  
3D Motion ◽  
Loading Rates ◽  
Tendon Pathology ◽  
3D Motion Capture

AbstractRecent epidemiological analyses in fencing have shown that injuries and pain linked specifically to fencing training/competition were evident in 92.8% of fencers. Specifically the prevalence of Achilles tendon pathology has increased substantially in recent years, and males have been identified as being at greater risk of Achilles tendon injury compared to their female counterparts. This study aimed to examine gender differences in Achilles tendon loading during the fencing lunge.Achilles tendon load was obtained from eight male and eight female club level epee fencers using a 3D motion capture system and force platform information as they completed simulated lunges. Independent t-tests were performed on the data to determine whether differences existed.The results show that males were associated with significantly greater Achilles tendon loading rates in comparison to females.This suggests that male fencers may be at greater risk from Achilles tendon pathology as a function of fencing training/ competition.

scholarly journals Molecular Mechanisms of Fetal Tendon Regeneration Versus Adult Fibrous Repair

2021 ◽  
Vol 22 (11) ◽  
pp. 5619
Author(s):  
Iris Ribitsch ◽  
Andrea Bileck ◽  
Alexander D. Aldoshin ◽  
Maciej M. Kańduła ◽  
Rupert L. Mayer ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Molecular Mechanisms ◽  
Unmet Need ◽  
Tendon Healing ◽  
Tendon Injury ◽  
Inflammatory Factors ◽  
Large Animal ◽  
Post Injury ◽  
Tendon Regeneration ◽  
Large Animal Models

Tendinopathies are painful, disabling conditions that afflict 25% of the adult human population. Filling an unmet need for realistic large-animal models, we here present an ovine model of tendon injury for the comparative study of adult scarring repair and fetal regeneration. Complete regeneration of the fetal tendon within 28 days is demonstrated, while adult tendon defects remained macroscopically and histologically evident five months post-injury. In addition to a comprehensive histological assessment, proteome analyses of secretomes were performed. Confirming histological data, a specific and pronounced inflammation accompanied by activation of neutrophils in adult tendon defects was observed, corroborated by the significant up-regulation of pro-inflammatory factors, neutrophil attracting chemokines, the release of potentially tissue-damaging antimicrobial and extracellular matrix-degrading enzymes, and a response to oxidative stress. In contrast, secreted proteins of injured fetal tendons included proteins initiating the resolution of inflammation or promoting functional extracellular matrix production. These results demonstrate the power and relevance of our novel ovine fetal tendon regeneration model, which thus promises to accelerate research in the field. First insights from the model already support our molecular understanding of successful fetal tendon healing processes and may guide improved therapeutic strategies.

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