scholarly journals Sonication culture improves microbiological diagnosis of modular megaprostheses

2016 ◽  
Vol 35 (7) ◽  
pp. 1383-1387 ◽  
Author(s):  
Stephan E. Puchner ◽  
Kevin Döring ◽  
Kevin Staats ◽  
Christoph Böhler ◽  
Richard Lass ◽  
...  
Keyword(s):  
Microbiological Diagnosis ◽  
Sonication Culture

scholarly journals Pulmonary tuberculosis due to Mycobacterium microti: a study of six recent cases in France

2010 ◽  
Vol 59 (8) ◽  
pp. 984-989 ◽  
Author(s):  
G. Panteix ◽  
M. C. Gutierrez ◽  
M. L. Boschiroli ◽  
M. Rouviere ◽  
A. Plaidy ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Clinical Significance ◽  
Molecular Techniques ◽  
Clinical Samples ◽  
Microbiological Diagnosis ◽  
Review Of The Literature ◽  
Antituberculous Therapy ◽  
Clinical Illness ◽  
Mycobacterium Microti ◽  
Immunocompetent Patients

Human tuberculosis caused by Mycobacterium microti is rare, but its prevalence and clinical significance may have been underestimated. To the best of our knowledge, 21 cases have been reported in the literature in the last decade. We report six recent pulmonary cases caused by M. microti over a period of 5 years detected in French clinical mycobacteriology laboratories of the hospital network. Our data confirm the potential of M. microti to cause clinical illness in immunocompetent patients. M. microti grew slowly from specimens, delaying the final microbiological diagnosis. Therefore, patients with tuberculosis caused by M. microti could benefit from the use of rapid diagnostic molecular techniques directly on clinical samples. From a review of the literature and this study, a classical antituberculous therapy seems effective in treating patients with M. microti disease.

Microbiological Diagnosis of Fungal Infections

2018 ◽  
pp. 96-105
Author(s):  
Gagandeep Singh ◽  
Immaculata Xess
Keyword(s):  
Fungal Infections ◽  
Microbiological Diagnosis

scholarly journals Sonication Culture of Antimicrobial Agent-Containing Cement Spacers Removed during Staged Revisions for Arthroplasty Infection

2018 ◽  
Vol 57 (2) ◽  
Author(s):  
Eric Gomez-Urena ◽  
Rafael J. Sierra ◽  
Kerryl E. Greenwood-Quiantance ◽  
Melissa J. Karau ◽  
James M. Steckelberg ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Antimicrobial Agent ◽  
Persistent Infection ◽  
Joint Infection ◽  
Revision Arthroplasty ◽  
Similar Proportion ◽  
Tissue Cultures ◽  
Periprosthetic Tissue ◽  
Prosthetic Joint ◽  
Sonication Culture

ABSTRACT Diagnosis of persistent infection at the time of reimplantation for staged revision of infected arthroplasties is challenging. Implant sonication culture for the diagnosis of prosthetic joint infection (PJI) has improved sensitivity compared to standard periprosthetic tissue culture. We report our experience with periprosthetic tissue culture and sonication culture of antimicrobial agent-containing cement spacers (ACSs) collected during second stages of staged revisions for arthroplasty infection. We studied 87 ACSs from 66 patients undergoing two-stage revision arthroplasty for PJI submitted for sonication culture, along with conventional periprosthetic tissue cultures. Two or more positive periprosthetic tissue cultures with the same organism were considered a positive tissue culture. For sonication culture, ≥20 CFU of bacteria per 10 ml of sonicate fluid was considered positive. The sensitivity and specificity of periprosthetic tissue and ACS sonication culture in detecting persistent infection, as well as their association with outcome, were assessed. Persistent infection occurred in 26% of cases. Periprosthetic tissue and sonicate fluid culture had specificities of 96.3 and 100% (P = 0.50), respectively, and sensitivities of 31.6 and 26.3% (P = 1.00), respectively, for the diagnosis of persistent infection. Thirteen subjects deemed not to have persistent infection at time of reimplantation and who had negative periprosthetic tissue and sonicate fluid cultures subsequently developed overt infection. Sonication culture of cement spacers identifies a similar proportion of patients with persistent infection during staged revisions, as detected by periprosthetic tissue cultures; both have low sensitivities to detect persistent infection.

scholarly journals A Preliminary Report of Predisposing Factors and Predominant Microbiological Diagnosis of Corneal Ulcers Seen at the Federal Teaching Hospital Abakaliki, Nigeria

2019 ◽  
Vol 09 (02) ◽  
pp. 94-104
Author(s):  
Chimdia E. Ogbonnaya ◽  
Favoured Walter-Ugwuocha ◽  
Edak Ezeanosike ◽  
Chinyelu N. Ezisi ◽  
Boniface N. Ukwah ◽  
...  
Keyword(s):  
Teaching Hospital ◽  
Preliminary Report ◽  
Predisposing Factors ◽  
Microbiological Diagnosis ◽  
Corneal Ulcers

scholarly journals Microbiological Diagnosis of Brucellosis

1998 ◽  
Vol 36 (6) ◽  
pp. 1819-1819 ◽  
Author(s):  
Marı́a Pı́a Roiz ◽  
Francisco Galo Peralta ◽  
Reina Valle ◽  
Rafael Arjona
Keyword(s):  
Microbiological Diagnosis

scholarly journals Association of Cartridge Based Nucleic Acid Amplification Test (CBNAAT) Grading on the Basis of Cycle Threshold Value with Conventional Microbiological Diagnosis in Pediatric Tuberculosis

2021 ◽  
Vol 7 (2) ◽  
pp. 72-77
Author(s):  
Bineeta Kashyap ◽  
Neha Gupta ◽  
Pooja Dewan ◽  
NP Singh ◽  
Ashwani Khanna
Keyword(s):  
Nucleic Acid ◽  
Positive Association ◽  
Threshold Value ◽  
Nucleic Acid Amplification Test ◽  
Nucleic Acid Amplification ◽  
Microbiological Diagnosis ◽  
Significant Positive Association ◽  
Cycle Threshold ◽  
Culture Positivity ◽  
Pediatric Tuberculosis

Background: Microbiological confirmation of tuberculosis disease in children remains difficult due to paucibacillary disease and inability to obtain optimal samples. Recently introduced Cartridge based nucleic acid amplification test (CBNAAT) has improved microbiological diagnosis in pediatric tuberculosis. Objectives: We aimed to study association of CBNAAT grading based on cycle threshold value with conventional microbiological diagnosis. Methodology: This prospective study was conducted over a period from November 2016 to October 2017 in the Departments of Microbiology and Pediatrics, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi. CBNAAT positive pediatric TB cases ≤12 years were recruited and subjected to Ziehl-Neelsen staining for acid fast bacilli (AFB) & culture on Lowenstein Jensen medium. CBNAAT positivity was graded based on cycle threshold value: very low, low, medium and high. Results: Smear and culture positivity was highest (100%) among specimens with high positive CBNAAT result based on CT value. Time to culture positivity was inversely related to CBNAAT grading (p=0.000). Conclusion: CBNAAT grading has significant positive association with smear and culture positivity. Bangladesh Journal of Infectious Diseases 2020;7(2):72-77

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