scholarly journals Repair of rat calvarial defects using Si-doped hydroxyapatite scaffolds loaded with a bone morphogenetic protein-2-related peptide

2016 ◽  
Vol 34 (11) ◽  
pp. 1874-1882 ◽  
Author(s):  
Wei Cui ◽  
Guangfei Sun ◽  
Yanzhen Qu ◽  
Yi Xiong ◽  
Tingfang Sun ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Morphogenetic Protein 2 ◽  
Related Peptide ◽  
Morphogenetic Protein ◽  
Calvarial Defects ◽  
Si Doped

scholarly journals Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2

2020 ◽  
Vol 21 (17) ◽  
pp. 6425
Author(s):  
Songhyun Lim ◽  
Hao-Zhen Lyu ◽  
Ju-Ro Lee ◽  
Shi Huan Han ◽  
Jae Hyup Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Umbilical Cord ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Calvarial Defects ◽  
In Vivo Bone Formation

Recombinant human bone morphogenetic protein 2 (rhBMP-2) is one of the most potent osteogenic factors used to treat bone loss. However, at higher doses, rhBMP-2 does not necessarily increase bone formation but rather increases the incidence of adverse side effects. Here, we investigated whether umbilical cord mesenchymal stem cell (UCMSC)-derived nanovesicles (NVs) further increase the in vivo bone formation at high doses of rhBMP-2. In the presence of UCMSC-derived NVs, proliferation, migration, and tube formation of human umbilical vein endothelial cells were stimulated in vitro. Furthermore, migration and osteogenesis of human bone marrow-derived mesenchymal stem cells were stimulated. To examine the efficacy of UCMSC-derived NVs on in vivo bone formation, collagen sponges soaked with rhBMP-2 and UCMSC-derived NVs were used in athymic nude mice with calvarial defects. At a high rhBMP-2 dosage (500 ng/mL), UCMSC-derived NVs significantly promoted bone formation in calvarial defects; however, the UCMSC-derived NVs alone did not induce in vivo bone formation. Our results indicate that UCMSC-derived NVs can potentiate the bone formation efficacy of rhBMP-2 at a high dosage.

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