Differential analysis of peripheral blood- and bone marrow-derived endothelial progenitor cells for enhanced vascularization in bone tissue engineering

2012 ◽  
Vol 30 (9) ◽  
pp. 1507-1515 ◽  
Author(s):  
Ami R. Amini ◽  
Cato T. Laurencin ◽  
Syam P. Nukavarapu
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Progenitor Cells ◽  
Bone Tissue ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Differential Analysis ◽  
Endothelial Progenitor

The Use of Endothelial Progenitor Cells for Prevascularized Bone Tissue Engineering

2009 ◽  
Vol 15 (8) ◽  
pp. 2015-2027 ◽  
Author(s):  
Jeroen Rouwkema ◽  
Peter E. Westerweel ◽  
Jan de Boer ◽  
Marianne C. Verhaar ◽  
Clemens A. van Blitterswijk
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Progenitor Cells ◽  
Bone Tissue ◽  
Endothelial Progenitor Cells ◽  
Endothelial Progenitor

miR-126 modulates angiogenic growth parameters of peripheral blood endothelial progenitor cells

2015 ◽  
Vol 396 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Sebastian M. Goerke ◽  
Lena S. Kiefer ◽  
G. Björn Stark ◽  
Filip Simunovic ◽  
Günter Finkenzeller
Keyword(s):  
Tissue Engineering ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Growth Parameters ◽  
Therapeutic Option ◽  
Tube Formation ◽  
Loss Of Function ◽  
Endothelial Progenitor

Abstract Vascularization plays an important role in tissue engineering applications. It is known that implantation of differentiated endothelial cells or endothelial progenitor cells (EPCs) from cord blood (cbEPCs) gives rise to the formation of a complex functional neovasculature, whereas EPCs isolated from peripheral blood (pbEPCs) have a limited capability to form blood vessels upon implantation. MicroRNA-126 (miR-126) has been shown to have pro-angiogenic effects in vivo. In this study, we investigated whether modulation of miR-126 expression in pbEPCs may alter their angiogenic properties. Gain of function and loss of function experiments revealed that miR-126 has anti-angiogenic effects in pbEPCs. Overexpression of miR-126 resulted in decreased proliferation, migration, invasion and tube formation, while inhibition of miR-126 induced the opposite effects. However, modulation of miR-126 expression did not influence apoptotic susceptibility of pbEPCs. This study provides evidence that inhibition of miR-126 improves angiogenesis-related growth parameters in pbEPCs and may represent a therapeutic option to ameliorate the angiogenic and vasculogenic properties of pbEPCs.

scholarly journals Ectopic Bone Tissue Engineering in Mice Using Human Gingiva or Bone Marrow-Derived Stromal/Progenitor Cells in Scaffold-Hydrogel Constructs

2021 ◽  
Vol 9 ◽  
Author(s):  
Siddharth Shanbhag ◽  
Carina Kampleitner ◽  
Samih Mohamed-Ahmed ◽  
Mohammed Ahmad Yassin ◽  
Harsh Dongre ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Bone Tissue Engineering ◽  
Progenitor Cells ◽  
Bone Tissue ◽  
Trimethylene Carbonate ◽  
Micro Computed Tomography ◽  
Spheroid Culture ◽  
Ectopic Mineralization ◽  
2D And 3D

Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor cells (GPC) represent a less invasive alternative to bone marrow MSC (BMSC) for clinical applications. The aim of this study was to test the in vivo bone forming potential of human GPC and BMSC cultured as 3D spheroids or dissociated cells (2D). 2D and 3D cells encapsulated in constructs of human platelet lysate hydrogels (HPLG) and 3D-printed poly (L-lactide-co-trimethylene carbonate) scaffolds (HPLG-PLATMC) were implanted subcutaneously in nude mice; cell-free HPLG-PLATMC constructs served as a control. Mineralization was assessed using micro-computed tomography (µCT), histology, scanning electron microscopy (SEM) and in situ hybridization (ISH). After 4–8 weeks, µCT revealed greater mineralization in 3D-BMSC vs. 2D-BMSC and 3D-GPC (p < 0.05), and a similar trend in 2D-GPC vs. 2D-BMSC (p > 0.05). After 8 weeks, greater mineralization was observed in cell-free constructs vs. all 2D- and 3D-cell groups (p < 0.05). Histology and SEM revealed an irregular but similar mineralization pattern in all groups. ISH revealed similar numbers of 2D and 3D BMSC/GPC within and/or surrounding the mineralized areas. In summary, spheroid culture promoted ectopic mineralization in constructs of BMSC, while constructs of dissociated GPC and BMSC performed similarly. The combination of HPLG and PLATMC represents a promising scaffold for bone tissue engineering applications.

CD16 antigen is a positive marker of peripheral blood-derived early endothelial progenitor cells

2010 ◽  
Vol 93 (1) ◽  
pp. 123-125 ◽  
Author(s):  
Takashi Kimura ◽  
Hirao Kohno ◽  
Yoshikazu Matsuoka ◽  
Ryusuke Nakatsuka ◽  
Yutaka Sasaki ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Peripheral Blood ◽  
Endothelial Progenitor ◽  
Cd16 Antigen
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