In vivo degradation and new bone formation of calcium phosphate cement-gelatin powder composite related to macroporosity after in situ gelatin degradation

2011 ◽  
Vol 30 (7) ◽  
pp. 1103-1111 ◽  
Author(s):  
Akihiro Kasuya ◽  
Satoshi Sobajima ◽  
Mitsuo Kinoshita
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Calcium Phosphate Cement ◽  
Powder Composite ◽  
New Bone Formation ◽  
In Vivo Degradation

In Vitro and In Vivo Evaluation Of Calcium Phosphate Bone Graft Substitutes

2010 ◽  
Vol 654-656 ◽  
pp. 2065-2070
Author(s):  
Ho Yeon Song ◽  
Young Hee Kim ◽  
Jyoti M. Anirban ◽  
In Seon Byun ◽  
Kyung A Kwak ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Graft ◽  
Cellular Proliferation ◽  
New Bone Formation ◽  
Hydroxy Apatite ◽  
Defect Sites ◽  
Bone Graft Substitutes

Calcium phosphate ceramics such as hydroxy apatite (HA), β-tricalcium phosphate (β-TCP) and bicalcium phosphate (BCP) have been used as a bone graft biomaterial because of their good biocompatibility and similarity of chemical composition to natural bones. To increase the mechanical and osteoconductive properties, the granules and spongy type porous bone graft substitutes were prepared by fibrous monolithic process and polyurethane foam replica methods, respectively. The pore sizes obtained using these approaches ranged between 100-600 µm. The cytotoxicity, cellular proliferation, differentiation and ECM deposition on the bone graft substitutes were observed by SEM and confocal microscopy. Moreover, the scaffolds were implanted in the rabbit femur. New bone formation and biodegradation of bone graft were observed through follow-up X-ray, micro-CT analysis and histological findings. After several months (2, 3, 6, 12 and 24 months) of implantation, new bone formation and ingrowths were observed in defect sites of the animal by CaP ceramics and 2 to 3 times higher bone ingrowths were confirmed than that of the normal trabecular bones in terms of total bone volume (BV).

Effect of Particle Size on New Bone Formation In Vivo Using Calcium Phopshate Glass

2007 ◽  
Vol 330-332 ◽  
pp. 165-168
Author(s):  
Hyun Ju Moon ◽  
Racquel Z. LeGeros ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
Seong Ho Choi ◽  
...  
Keyword(s):  
Particle Size ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Phosphate Glass ◽  
Glass Powder ◽  
New Bone Formation ◽  
Sprague Dawley ◽  
Constant Weight ◽  
Calvarial Defects

The purpose of this study was to compare the bone regenerative effect of calcium phosphate glass according to the particle size in vivo. We prepared two different sizes, that is 400 μm and 40 μm, of calcium phosphate glass powder using the system CaO-CaF2-P2O5-MgO-ZnO. Critical-sized calvarial defects were created in 60 male Sprague-Dawley rats. The animals were divided into 3 groups of 20 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As controls, the defect was left empty. The rats were sacrificed 2 or 8 weeks after postsurgery, and the results were evaluated using histological as well as histomorphometrical studies. The particle size of the calcium phosphate was crucial; 400 μm particles promoted new bone formation, while 40 μm particles inhibited it because of severe inflammation.

scholarly journals The optimum zinc content in set calcium phosphate cement for promoting bone formation in vivo

2009 ◽  
Vol 29 (3) ◽  
pp. 969-975 ◽  
Author(s):  
Xia Li ◽  
Yu Sogo ◽  
Atsuo Ito ◽  
Hirotaka Mutsuzaki ◽  
Naoyuki Ochiai ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Calcium Phosphate Cement ◽  
Zinc Content

Vascularization of plastic calcium phosphate cement in vivo induced by in-situ-generated hollow channels

2016 ◽  
Vol 68 ◽  
pp. 153-162 ◽  
Author(s):  
Tao Yu ◽  
Chao Dong ◽  
Zhonghua Shen ◽  
Yan Chen ◽  
Bo Yu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement

scholarly journals Ex Vivo and In Vivo Analyses of Novel 3D-Printed Bone Substitute Scaffolds Incorporating Biphasic Calcium Phosphate Granules for Bone Regeneration

2021 ◽  
Vol 22 (7) ◽  
pp. 3588
Author(s):  
Franciska Oberdiek ◽  
Carlos Ivan Vargas ◽  
Patrick Rider ◽  
Milijana Batinic ◽  
Oliver Görke ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Biphasic Calcium Phosphate ◽  
Ex Vivo ◽  
Proper Function ◽  
New Bone Formation ◽  
3D Printed ◽  
Implantation Model

(1) Background: The aim of this study was examining the ex vivo and in vivo properties of a composite made from polycaprolactone (PCL) and biphasic calcium phosphate (BCP) (synprint, ScientiFY GmbH) fabricated via fused deposition modelling (FDM); (2) Methods: Scaffolds were tested ex vivo for their mechanical properties using porous and solid designs. Subcutaneous implantation model analyzed the biocompatibility of PCL + BCP and PCL scaffolds. Calvaria implantation model analyzed the osteoconductive properties of PCL and PCL + BCP scaffolds compared to BCP as control group. Established histological, histopathological and histomorphometrical methods were performed to evaluate new bone formation.; (3) Results Mechanical testing demonstrated no significant differences between PCL and PCL + BCP for both designs. Similar biocompatibility was observed subcutaneously for PCL and PCL + BCP scaffolds. In the calvaria model, new bone formation was observed for all groups with largest new bone formation in the BCP group, followed by the PCL + BCP group, and the PCL group. This finding was influenced by the initial volume of biomaterial implanted and remaining volume after 90 days. All materials showed osteoconductive properties and PCL + BCP tailored the tissue responses towards higher cellular biodegradability. Moreover, this material combination led to a reduced swelling in PCL + BCP; (4) Conclusions: Altogether, the results show that the newly developed composite is biocompatible and leads to successful osteoconductive bone regeneration. The new biomaterial combines the structural stability provided by PCL with bioactive characteristics of BCP-based BSM. 3D-printed BSM provides an integration behavior in accordance with the concept of guided bone regeneration (GBR) by directing new bone growth for proper function and restoration.

Biocompatibility and Osteogenesis of Calcium Phosphate Cement Scaffolds for Bone Tissue Engineering

2008 ◽  
Vol 47-50 ◽  
pp. 1383-1386 ◽  
Author(s):  
Han Guo ◽  
Jie Wei ◽  
Hang Kong ◽  
Chang Sheng Liu ◽  
Ke Feng Pan
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
New Bone Formation ◽  
Negative Effects ◽  
Osteoblastic Phenotype ◽  
Proliferation And Differentiation ◽  
Good Biocompatibility

Porous calcium phosphate cement (CPC) scaffolds were successfully fabricated utilizing particle-leaching method. Mesenchymal stem cells (MSCs) were cultured, expanded and seeded on the scaffolds and the proliferation and differentiation of MSCs into osteoblastic phenotype were determined using MTT assay, ALP activity and ESEM. The results revealed that the CPC scaffolds were biocompatible and had no negative effects on the MSCs in vitro. The in vivo biocompatibility and osteogenicity of the scaffolds were investigated. Both pure scaffolds and MSCs/scaffold constructs were implanted in rabbit mandibles and studied histologically. The results showed that CPC scaffolds exhibited good biocompatibility and osteoconductivity. Moreover, the introduction of MSCs into the scaffolds dramatically enhanced the efficiency of new bone formation initially.

Microencapsulated rBMMSCs/calcium phosphate cement for bone formation in vivo

2014 ◽  
Vol 24 (1) ◽  
pp. 835-843 ◽  
Author(s):  
Juan Wang ◽  
Pengyan Qiao ◽  
Limin Dong ◽  
Fangfang Li ◽  
Tao Xu ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Bone Formation ◽  
Calcium Phosphate Cement

In vivo degradation of calcium phosphate cement incorporated into biodegradable microspheres

2010 ◽  
Vol 6 (6) ◽  
pp. 2200-2211 ◽  
Author(s):  
W.J.E.M. Habraken ◽  
H.B. Liao ◽  
Z. Zhang ◽  
J.G.C. Wolke ◽  
D.W. Grijpma ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Calcium Phosphate Cement ◽  
Biodegradable Microspheres ◽  
In Vivo Degradation

scholarly journals In Vivo Study for Clinical Application of Dental Stem Cell Therapy Incorporated with Dental Titanium Implants

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 381
Author(s):  
Hyunmin Choi ◽  
Kyu-Hyung Park ◽  
Narae Jung ◽  
June-Sung Shim ◽  
Hong-Seok Moon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Formation ◽  
Alveolar Bone ◽  
Human Mesenchymal Stem Cells ◽  
Titanium Implants ◽  
Osteogenic Potential ◽  
Induction Medium ◽  
Radiographic Analysis ◽  
New Bone Formation

The aim of this study was to investigate the behavior of dental-derived human mesenchymal stem cells (d-hMSCs) in response to differently surface-treated implants and to evaluate the effect of d-hMSCs on local osteogenesis around an implant in vivo. d-hMSCs derived from alveolar bone were established and cultured on machined, sandblasted and acid-etched (SLA)-treated titanium discs with and without osteogenic induction medium. Their morphological and osteogenic potential was assessed by scanning electron microscopy (SEM) and real-time polymerase chain reaction (RT-PCR) via mixing of 5 × 106 of d-hMSCs with 1 mL of Metrigel and 20 μL of gel-cell mixture, which was dispensed into the defect followed by the placement of customized mini-implants (machined, SLA-treated implants) in New Zealand white rabbits. Following healing periods of 2 weeks and 12 weeks, the obtained samples in each group were analyzed radiographically, histomorphometrically and immunohistochemically. The quantitative change in osteogenic differentiation of d-hMSCs was identified according to the type of surface treatment. Radiographic analysis revealed that an increase in new bone formation was statistically significant in the d-hMSCs group. Histomorphometric analysis was in accordance with radiographic analysis, showing the significantly increased new bone formation in the d-hMSCs group regardless of time of sacrifice. Human nuclei A was identified near the area where d-hMSCs were implanted but the level of expression was found to be decreased as time passed. Within the limitations of the present study, in this animal model, the transplantation of d-hMSCs enhanced the new bone formation around an implant and the survival and function of the stem cells was experimentally proven up to 12 weeks post-sacrifice.

Sign in / Sign up

Export Citation Format

Share Document