scholarly journals Combined effects of TNF-α, IL-1β, and HIF-1α on MMP-2 production in ACL fibroblasts under mechanical stretch: An in vitro study

2011 ◽  
Vol 29 (7) ◽  
pp. 1008-1014 ◽  
Author(s):  
Yequan Wang ◽  
Zhenyu Tang ◽  
Ruyue Xue ◽  
Gurinder K. Singh ◽  
Kunning Shi ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Mechanical Stretch ◽  
Vitro Study ◽  
Combined Effects ◽  
Tnf Α

A Possible Mechanism of Action of 17α-Hydroxyprogesterone Caproate: Enhanced IL-10 Production

2021 ◽  
Author(s):  
Christina J. Megli ◽  
Alisse Hauspurg ◽  
Raman Venkataramanan ◽  
Steve N. Caritis
Keyword(s):  
Mechanism Of Action ◽  
Cytokine Production ◽  
Plasma Concentrations ◽  
In Vitro Study ◽  
Vitro Study ◽  
Clinical Samples ◽  
Omega 3 ◽  
Tnf Α ◽  
Hydroxyprogesterone Caproate

Objective The rate of recurrent spontaneous preterm birth (PTB) was reduced by 33% in the Maternal-Fetal Medicine Unit (MFMU) Network trial of 17α-hydroxyprogesterone caproate (17-OHPC), but the mechanism of action, 17 years later, remains elusive. The robustness of the interleukin-10 (IL-10) response to lipopolysaccharide (LPS) stimulation of leukocytes in pregnant women with a prior PTB correlates with gestational age at delivery. This study sought to determine if there is a relationship between the concentration of 17-OHPC and response to LPS stimulation. Study Design We performed a secondary analysis of data from the Omega-3 MFMU trial which evaluated the effectiveness of omega-3 fatty acid supplementation in reducing recurrent PTB. We utilized previously characterized data from a subanalyses of the Omega-3 trial of IL-10 and tumor necrosis factor alpha (TNF-α) levels from peripheral blood mononuclear cells stimulated with LPS. Blood was obtained from enrolled women at 16 to 22 weeks' gestation (baseline) and 25 to 28 weeks' gestation (posttreatment). All women received 17-OHPC and plasma 17-OHPC concentrations were measured at 25 to 28 weeks' gestation. We analyzed these data to determine if there was a relationship between 17-OHPC concentration and cytokine production. We then performed an in vitro study to determine if 17-OHPC could directly alter cytokine production by THP-1-derived macrophages. Results In the clinical samples, we found that 17-OHPC plasma concentrations were correlated with the quantity of the LPS-stimulated production of IL-10. TNF-α production after LPS stimulation was unrelated to 17-OHPC concentration. In the in vitro study, we demonstrate a 17-OHPC concentration dependent increase in IL-10 production. Conclusion In women receiving 17-OHPC for PTB prevention, we demonstrate a relationship between plasma 17-OHPC and LPS-stimulated IL-10 production by circulating leukocytes. We also demonstrate that, in vitro, 17-OHPC treatment affects IL-10 production by LPS-stimulated macrophages. Collectively, these findings support an immunomodulatory mechanism of action of 17-OHPC in the prevention of recurrent PTB. Key Points

scholarly journals Anti-Ro and anti-La autoantibodies induce TNF-α production by human salivary gland cells: an in vitro study

Reumatismo ◽  
2011 ◽  
Vol 59 (3) ◽  
Author(s):  
M. Sisto ◽  
S. Lisi ◽  
M. D'Amore ◽  
V. Mitolo ◽  
P. Scagliusi
Keyword(s):  
Salivary Gland ◽  
In Vitro Study ◽  
Vitro Study ◽  
Human Salivary Gland ◽  
Gland Cells ◽  
Salivary Gland Cells ◽  
Tnf Α

Curcumin-loaded niosomes downregulate mRNA expression of pro-inflammatory markers involved in asthma: an in vitro study

Nanomedicine ◽  
2020 ◽  
Vol 15 (30) ◽  
pp. 2955-2970
Author(s):  
Jin-Ying Wong ◽  
Zhao Yin Ng ◽  
Meenu Mehta ◽  
Shakti D Shukla ◽  
Jithendra Panneerselvam ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Inflammatory Markers ◽  
In Vitro Release ◽  
Physicochemical Characterization ◽  
In Vitro Study ◽  
Physicochemical Characteristics ◽  
Vitro Study ◽  
Tnf Α ◽  
Vitro Release

Aim: In this study, curcumin was encapsulated in niosomes (Nio-Curc) to increase its effectiveness for the treatment of asthma. Materials & methods: The formulation underwent various physicochemical characterization experiments, an in vitro release study, molecular simulations and was evaluated for in vitro anti-inflammatory activity. Results: Results showed that Nio-Curc had a mean particle size of 284.93 ± 14.27 nm, zeta potential of -46.93 and encapsulation efficacy of 99.62%, which demonstrates optimized physicochemical characteristics. Curcumin release in vitro could be sustained for up to 24 h. Additionally, Nio-Curc effectively reduced mRNA transcript expression of pro-inflammatory markers; IL-6, IL-8, IL-1β and TNF-α in immortalized human airway basal cell line (BCi-NS1.1). Conclusion: In this study, we have demonstrated that Nio-Curc mitigated the mRNA expression of pro-inflammatory markers in an in vitro study, which could be applied to treatment of asthma with further studies.

Mutagenic and cytotoxic potential of Endosulfan and Lambda-cyhalothrin — In vitro study describing individual and combined effects of pesticides

2014 ◽  
Vol 26 (7) ◽  
pp. 1471-1479 ◽  
Author(s):  
Umber Saleem ◽  
Sohail Ejaz ◽  
Muhammad Ashraf ◽  
Muhammad Ovais Omer ◽  
Imran Altaf ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
Combined Effects ◽  
Cytotoxic Potential ◽  
Lambda Cyhalothrin

scholarly journals Differential MMP-2 activity of ligament cells under mechanical stretch injury: An in vitro study on human ACL and MCL fibroblasts

2005 ◽  
Vol 23 (4) ◽  
pp. 949-957 ◽  
Author(s):  
David Zhou ◽  
Hwa Sung Lee ◽  
Francisco Villarreal ◽  
Annabelle Teng ◽  
Erick Lu ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Mechanical Stretch ◽  
Vitro Study ◽  
Stretch Injury
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