scholarly journals Radial shock waves effectively introduced NF-kappa B decoy into rat achilles tendon cells in vitro

2010 ◽  
Vol 28 (8) ◽  
pp. 1078-1083 ◽  
Author(s):  
Kaori Sugioka ◽  
Koichi Nakagawa ◽  
Ryo Murata ◽  
Nobuyasu Ochiai ◽  
Takahisa Sasho ◽  
...  
Keyword(s):  
Shock Waves ◽  
Achilles Tendon ◽  
Nf Kappa B ◽  
Tendon Cells

Biomechanical properties of different techniques used in vitro for suturing mid-substance Achilles tendon ruptures

2017 ◽  
Vol 50 ◽  
pp. 78-83 ◽  
Author(s):  
Carlos De la Fuente ◽  
Carlos Cruz-Montecinos ◽  
Helen L. Schimidt ◽  
Hugo Henríquez ◽  
Sebastián Ruidiaz ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Biomechanical Properties ◽  
Tendon Ruptures

In vitro Cholesterol Gallstone Dissolution after Fragmentation with Shock Waves

Digestion ◽  
1986 ◽  
Vol 34 (1) ◽  
pp. 51-59 ◽  
Author(s):  
M. Neubrand ◽  
T. Sauerbruch ◽  
F. Stellaard ◽  
G. Paumgartner
Keyword(s):  
Shock Waves ◽  
Cholesterol Gallstone ◽  
Gallstone Dissolution

scholarly journals Dexamethasone decreases substance P expression in human tendon cells: an in vitro study

Rheumatology ◽  
2014 ◽  
Vol 54 (2) ◽  
pp. 318-323 ◽  
Author(s):  
R. Mousavizadeh ◽  
L. Backman ◽  
R. G. McCormack ◽  
A. Scott
Keyword(s):  
Substance P ◽  
In Vitro Study ◽  
Vitro Study ◽  
Tendon Cells ◽  
Human Tendon

N-terminal DNA-binding domains contribute to differential DNA-binding specificities of NF-kappa B p50 and p65

1993 ◽  
Vol 13 (2) ◽  
pp. 852-860
Author(s):  
M B Toledano ◽  
D Ghosh ◽  
F Trinh ◽  
W J Leonard
Keyword(s):  
Dna Binding ◽  
Point Mutations ◽  
Terminal Region ◽  
Sequence Motif ◽  
Structural Determinants ◽  
Nf Kappa B ◽  
Dna Binding Domains ◽  
Binding Domains ◽  
Dna Binding Specificity

We previously reported that either oxidation or alkylation of NF-kappa B in vitro abrogates DNA binding. We used this phenomenon to help elucidate structural determinants of NF-kappa B binding. We now demonstrate that Cys-62 of NF-kappa B p50 mediates the redox effect and lies within an N-terminal region required for DNA binding but not for dimerization. Several point mutations in this region confer a transdominant negative binding phenotype to p50. The region is highly conserved in all Rel family proteins, and we have determined that it is also critical for DNA binding of NF-kappa B p65. Replacement of the N-terminal region of p65 with the corresponding region from p50 changes its DNA-binding specificity towards that of p50. These data suggest that the N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. We have defined within the N-terminal region a sequence motif, R(F/G)(R/K)YXCE, which is present in Rel family proteins and also in zinc finger proteins capable of binding to kappa B sites. The potential significance of this finding is discussed.

Functional characterization of the NF-kappa B p65 transcriptional activator and an alternatively spliced derivative

1992 ◽  
Vol 12 (2) ◽  
pp. 444-454
Author(s):  
S M Ruben ◽  
R Narayanan ◽  
J F Klement ◽  
C H Chen ◽  
C A Rosen
Keyword(s):  
Dna Binding ◽  
Complex Formation ◽  
Functional Characterization ◽  
Transcriptional Activator ◽  
Single Amino Acid ◽  
Nf Kappa B ◽  
Mobility Shift ◽  
Activation Domain ◽  
Transcriptional Activator Protein

The NF-kappa B transcription factor complex is composed of two proteins, designated p50 and p65, both having considerable homology to the product of the rel oncogene. We present evidence that the p65 subunit is a potent transcriptional activator in the apparent absence of the p50 subunit, consistent with in vitro results demonstrating that p65 can interact with DNA on its own. To identify the minimal activation domain, chimeric fusion proteins between the DNA binding domain of the yeast transcriptional activator protein GAL4 and regions of the carboxy terminus of p65 were constructed, and their transcriptional activity was assessed by using a GAL4 upstream activation sequence-driven promoter-chloramphenicol acetyltransferase fusion. This analysis suggests that the boundaries of the activation domain lie between amino acids 415 and 550. Moreover, single amino acid changes within residues 435 to 459 greatly diminished activation. Similar to other activation domains, this region contains a leucine zipper-like motif as well as an overall net negative charge. To identify those residues essential for DNA binding, we made use of a naturally occurring derivative of p65, lacking residues 222 to 231 (hereafter referred to as p65 delta), and produced via an alternative splice site. Gel mobility shift analysis using bacterially expressed p65, p65 delta, and various mutants indicates that residues 222 to 231 are important for binding to kappa B DNA. Coimmunoprecipitation analysis suggests that these residues likely contribute to the multimerization function required for homomeric complex formation or heteromeric complex formation with p50 in that no association of p65 delta with itself or with p50 was evident. However, p65 delta was able to form weak heteromeric complexes with p65 that were greatly reduced in their ability to bind DNA. On the basis of these findings, we suggest that subtle changes within the proposed multimerization domain can elicit different effects with the individual Rel-related proteins and that a potential role of p65 delta may be to negatively regulate NF-kappa B function through formation of nonfunctional heteromeric complexes.

The Effect of Shock Waves on Human Prostatic Carcinoma Cells In Vitro

1989 ◽  
pp. 91-95
Author(s):  
Issac Kaver ◽  
Warren W. Koontz ◽  
John D. Wilson ◽  
John M. Guice ◽  
M. J. Vernon Smith
Keyword(s):  
Shock Waves ◽  
Prostatic Carcinoma ◽  
Carcinoma Cells

The In Vitro and In Vivo Effects of Extracorporeal Shock Waves on Tumor Cells

1988 ◽  
pp. 351-355
Author(s):  
Gerhard J. Fuchs ◽  
Randall F. Randazzo ◽  
Anna M. Fuchs ◽  
Arnulf Stenzl ◽  
Christian G. Chaussy
Keyword(s):  
Shock Waves ◽  
Tumor Cells ◽  
Extracorporeal Shock Waves ◽  
In Vivo Effects

scholarly journals A Novel Kartogenin-Releasing Polymer Scaffold Promotes Wounded Rat Achilles Tendon Enthesis Healing

2018 ◽  
Vol 3 (3) ◽  
pp. 2473011418S0053
Author(s):  
Jianying Zhang ◽  
Daibang Nie ◽  
Guangyi Zhao ◽  
Susheng Tan ◽  
MaCalus Hogan ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Saline Group ◽  
Future Research ◽  
Dependent Manner ◽  
Polymer Scaffold ◽  
Normal Medium ◽  
Post Surgery ◽  
Tendon Stem Cells

Category: Hindfoot Introduction/Purpose: Entheses have a special fibrocartilage transition zone where tendons and ligaments attach to bone. Enthesis injury is very common, and the reattachment of tendon to bone is a great challenge because healing takes place between a soft tissue (tendon) and a hard tissue (bone). We have now developed a kartogene (KGN)-containing polymer scaffold (KGN-P) that can precisely deliver KGN to damaged enthesis area. The effects of the KGN-containing polymer on the healing of wounded TBJ were investigated in vitro and in vivo. Methods: The proliferation and chondrogenesis of rat Achilles tendon stem cells (TSCs) grown in four conditions were measured: normal medium (Control); normal medium with 100 nM KGN (KGN); lysine diisocyanate (LDI)-glycerol scaffold with normal medium (LDI-P); LDI-glycerol-KGN scaffold with normal medium (KGN-P).A wound (1 mm) was created on each hind leg Achilles enthesis of all 8 rats (3 months old). The wounds were then treated either with 10 ul saline (Wound); or 10 ul of 10 uM KGN (KGN); or LDI polymer scaffold (LDI-P); or KGN-containing polymer scaffold (KGN-P). The rats were sacrificed on day 15 and 30 post-surgery, and their Achilles entheses were collected for gross inspection and histochemical analysis. Results: KGN-containing polymers have sponge-like structures (Fig. 1A-D), and release KGN in a time- and temperature-dependent manner (Fig. 1E). KGN-P scaffold induced chondrogenesis of TSCs (Fig. 2D, 2H) without changing cell proliferation (Fig. 2I), and enhanced fibrocartilage-like tissue formation (Fig. 3E). KGN (Fig. 3C) and LDI-P (Fig. 3D) treated groups exhibited unhealed wound areas as in saline group (Fig. 3B). Finally, KGN-P and KGN treated rat TSCs underwent chondrogenesis by upregulating collagen II, aggrecan, and SOX-9 expression (Fig. 3F). Conclusion: Our results showed that KGN-containing polymer scaffold enhanced wounded enthesis healing by inducing TSC chondrogenesis and promoting the formation of the fibrocartilage in the wound site. The KGN-P may be used for regeneration of wounded entheses in clinical settings. Future research will focus on optimizing KGN concentration and releasing rate in the polymer scaffold during enthesis healing.

1998 William J. Stickel Gold Award. The effects of extrinsic muscle forces on the forefoot-to-rearfoot loading relationship in vitro. Tibia and Achilles tendon

1998 ◽  
Vol 88 (10) ◽  
pp. 471-482 ◽  
Author(s):  
ED Ward ◽  
RD Phillips ◽  
PE Patterson ◽  
GJ Werkhoven
Keyword(s):  
Achilles Tendon ◽  
Gait Cycle ◽  
Axial Loading ◽  
Muscular Activity ◽  
Linear Increase ◽  
Muscle Forces ◽  
Limited Data ◽  
Loading System ◽  
Inclination Angles

The effects of muscular activity on the distribution of forces under the foot, as well as within the foot, are of great importance for determining the mechanisms of foot pathologies. Limited data exist concerning muscle forces during the gait cycle and the effects of muscle forces conveyed to the ground-reactive forces of the foot. The authors developed a cadaveric loading system to determine the effects of force applied to the Achilles tendon on the forefoot-to-rearfoot loading relationship in eight cadaveric specimens. The study indicated that, during axial loading of the tibia, force was inherently transferred from the rearfoot to the forefoot. However, the observed forefoot-to-rearfoot loading relationship did not match the predicted loading relationship from a rigid-body diagram, as would be observed in a class I lever. The results indicated that, as the force was increased on the Achilles tendon, the change in loads on the forefoot and rearfoot was not linear. Specimens with calcaneal inclination angles greater than 20 degrees demonstrated a more linear increase as compared with those with inclination angles less than 20 degrees.

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